EP0032134A2
Expired / Lapsed
EP
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DNA sequences, recombinant DNA molecules and processes for producing human interferon-alpha like polypeptides
Assignee: Biogen Inc
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Filing: 1981-01-07
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Granted: —
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Expires: 2001-01-07
Abstract
Abstract DNA sequences, recombinant DNA molecules and hosts transformed with them which produce polypeptides displaying a biological or immunological activity of human interferon, the genes coding for these polypeptides and methods of making and using these molecules, hosts, genes and polypeptides. The DNA sequences are characterized in that they code for a polypeptide displaying a biological or immunological activity of human interferon. In appropriate hosts these DNA sequences and recombinant DNA molecules permit the production and identification of genes and polypeptides displaying a biological or immunological activity of human interferon and their use in antiviral and antitumor or anticancer agents.
Inventors
- 👤 Charles Weissmann
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 19:12
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/02; A; A61; A61P; A61P31/00; A61P31/12; A61P35/00; A61P43/00; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/56; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/811; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
103 words
A DNA sequence selected from the group consisting of the DNA inserts of Z-pBR322(Pst)/HcIF-4c, Z-pBR322(Pst)/HcIF-2h, Z-pBR322(Pst)/HcIF-SN35, Z-pBR322(Pst)/ HcIF-SN42, Z-pKT287(Pst)/HcIF-2h-AH6, DNA sequences which hybridize to any of the foregoing DNA inserts, DNA sequences, from whatever source obtained, including natural, synthetic or semi-synthetic sources, related by mutation, including single or multiple, base substitutions, deletions, insertions and inversions to any of the foregoing DNA sequences or inserts, and DNA sequences comprising sequences of codons which on expression code for a polypeptide displaying similar immunological or biological activity to a polypeptide coded for on expression of the codons of any of the foregoing DNA sequences and inserts.
Claim 2
Dependent Claim
103 words
A DNA sequence selected from the group consisting of the DNA inserts of Z-pBR322(Pst)/HcIF-4c, Z-pBR322(Pst)/HcIF-2h, Z-pBR322(Pst)/HcIF-SN35, Z-pBR322(Pst)/ HcIF-SN42, Z-pKT287(Pst)/HcIF-2h-AH6, DNA sequences which hybridize to any of the foregoing DNA inserts, DNA sequences, from whatever source obtained, including natural, synthetic or semi-synthetic sources, related by mutation, including single or multiple, base substitutions, deletions, insertions and inversions to any of the foregoing DNA sequences or inserts, and DNA sequences comprising sequences of codons which on expression code for a polypeptide displaying similar immunological or biological activity to a polypeptide coded for on expression of the codons of any of the foregoing DNA sequences and inserts.
Claim 3
Dependent Claim
116 words
A DNA sequence according to claim 1 wherein said DNA sequence which hybridizes to said DNA insert is selected from the group consisting of the DNA inserts of Z-pBR322(Pst)/HcIF-II-206 or Z-pBR322 (Pst)/HcIF-SN35-AHL6, DNA sequences which hybridize to any of the foregoing DNA inserts, DNA sequences, from whatever source obtained, including natural, synthetic or semi-synthetic sources, related by mutation, including single or multiple, base substitutions, deletions, insertions and inversions to any of the foregoing DNA sequences or inserts, and DNA sequences comprising sequences of codons which on expression code for a polypeptide displaying similar immunological or biological activity to a polypeptide coded for on expression of the codons of any of the foregoing DNA sequences and inserts.
Claim 4
Dependent Claim
111 words
A DNA sequence according to claims 1 or 2 wherein said DNA sequence which hybridizes to said DNA insert is selected from the group consisting of Hif-chrl, Hif-chr3, Hif-chrl2, Hif-chr13, Hif-chrl6-ℓ, Hif-chr26, Hif-chr30, Hif-chr35, DNA sequences which hybridize to any of the foregoing DNA sequences, DNA sequences, from whatever source obtained, including natural, synthetic, or semi-synthetic sources, related by mutation, including single or multiple, base substitutions, deletions, insertions and inversions, to any of the foregoing DNA sequences and DNA sequences comprising sequences of codons which on expression code for a polypeptide similar in immunological or biological activity to a polypeptide coded for on expression of any of the foregoing DNA sequences.
Claim 5
Dependent Claim
105 words
A DNA sequence according to claim 1 or 2 wherein said DNA sequence which hybridizes to said DNA insert is selected from the group consisting of Hif-chrl9, Hif-chr27, DNA sequences which hybridize to any of the foregoing DNA sequences, DNA sequences, from whatever source obtained, including natural, synthetic, or semi-synthetic sources, related by mutation, including single or multiple, base substitutions, deletions, insertions and inversions, to any of the foregoing DNA sequences and DNA sequences comprising sequences of codons which on expression code for a polypeptide similar in immunological or biological activity to a polypeptide coded for on expression of any of the foregoing DNA sequences.
Claim 6
Dependent Claim
43 words
A DNA sequence according to any one of claims 1 to 4 selected from the group consisting of DNA sequences of the formula: and fragments and derivatives thereof, said fragments and derivatives coding for polypeptides displaying an immunological or biological activity of IFN-a.
Claim 7
Dependent Claim
42 words
A DNA sequence according to any one of claims 1 to 4 selected from the group consisting of DNA sequences of the formula: fragments and derivatives thereof, said fragments and derivatives coding for polypeptides displaying an immunological or biological activity of IFN-a.
Claim 8
Dependent Claim
43 words
A DNA sequence according to any one of claims 1 to 4 selected from the group consisting of DNA sequences of the formula: and fragments and derivatives thereof, said fragments and derivatives coding for polypeptides displaying an immunological or biological activity of IFN-a.
Claim 9
Dependent Claim
16 words
A recombinant DNA molecule comprising a DNA sequence according to any one of the preceeding claims.
Claim 10
Dependent Claim
20 words
The recombinant DNA molecule according to claim 8, wherein said DNA sequence is operatively linked to an expression control sequence.
Claim 11
Dependent Claim
61 words
A recombinant DNA molecule according to claim 9, wherein the expression control sequence is selected from the group consisting of a lac system, a β-lac system, a trp system, major operator and promotor regions of phage λ, the control region of fd coat protein, and other sequences which control the expression of genes of prokaryotic or eukaryotic cells and their viruses.
Claim 12
Dependent Claim
21 words
A recombinant DNA molecule according to claim 9 or 10 selected from the group consisting of C8-IFN-al, C8-IFN-a2, LAC-AUG(a2) and P-lac-AUG(a2).
Claim 13
Dependent Claim
19 words
A host transformed with at least one recombinant DNA molecule according to any one of claims 8 to 11.
Claim 14
Dependent Claim
36 words
The host of claim 12 selected from the group consisting of strains of E. coli, Pseudomonas, Bacillus subtilis, Bacillus stearothermophilus, other bacilli, yeasts, other fungi, mouse or other animal or plant hosts and human tissue cells.
Claim 15
Dependent Claim
38 words
The transformed host according to claim 12 or 13 selected from the group consisting of E. coli HB101 (Z-pBR322(Pst)/HcIF-4c), E. coli HB101 (Z-pBR322(Pst)/ HcIF-2h), E. coli HB101 (Z-pBR322(Pst)/HcIF-SN35), E. coli HB101 (Z-pBR322(Pst)/ HcIF-SN42), and E. coli HB101 (Z-pKT287(Pst)/HcIF-2h-AH6).
Claim 16
Dependent Claim
25 words
The transformed host according to claim 12 or 13 selected from the group consisting of E. coli HB101 (Z-pBR322(Pst)/HcIF-II-206) and E. coli HB101 (Z-pBR322(Pst)/ HcIF-SN35-AHL6).
Claim 17
Dependent Claim
26 words
The transformed host according to claim 12 or 13 selected from the group consisting of HchrIF-A, HchrIF-B, HchrIF-C, HchrIF-D, HchrIF-E, HchrIF-F, HchrIF-G, HchrIF-H, HchrIF-I, and HchrIF-J.
Claim 18
Dependent Claim
36 words
The transformed host according to claim 12 or 13 selected from the group consisting of E. coli DS410 (C8-IFN-αl), E. coli DS410 (C8-IFN-a2), E. coli DS410 (LAC-AUG(a2)), E. coli DS410 HB101 (βlac-AUG(α2)) and Mouse 3T3 (polynoma-Hif-chr35).
Claim 19
Dependent Claim
29 words
The transformed host according to claim 12 or 13 selected from the group consisting of HchrIF-K, HchrIF-L, HchrIF-M, HchrIF-N, HchrIF-O, HchrIF-P, HchrIF-Q and hosts transformed with Hif-chrl9 and Hif-chr27.
Claim 20
Dependent Claim
31 words
A polypeptide or fragments and derivatives thereof displaying an immunological or biological activity of human leukocyte interferon produced by the transformed host according to any one of claims 12 to 18.
Claim 21
Dependent Claim
20 words
A polypeptide that it is coded for by a DNA sequence according to any one of claims 1 to 7.
Claim 22
Dependent Claim
24 words
A polypeptide or fragments and derivatives thereof according to claim 19 or 20 selected from the group consisting of IFN-al, IFN-a2, IFN-a4a and IFN-a4b.
Claim 23
Dependent Claim
59 words
A polypeptide or fragments and derivatives thereof according to any one of claims 19 to 21 selected from the group consisting of polypeptides of the formula: and polypeptides from whatever source obtained related to any of the foregoing polypeptides by mutation, including single or multiple, base substitutions, deletions, insertions and inversions of the DNA sequences which code for them.
Claim 24
Dependent Claim
58 words
A polypeptide or fragments and derivatives thereof.according to any one of claims 19 to 21 selected from the group consisting of polypeptides of the formula: and polypeptides from whatever source obtained related to any of the foregoing polypeptides by mutation, including single or multiple, base substitutions, deletions, insertions and inversions of the DNA sequences which code for them.
Claim 25
Dependent Claim
59 words
A polypeptide or fragments and derivatives thereof according to any one of claims 19 to 21 selected from the group consisting of polypeptides of the formula: and polypeptides from whatever source obtained related to any of the foregoing polypeptides by mutation, including single or multiple, base substitutions, deletions, insertions and inversions of the DNA sequences which code for them.
Claim 26
Independent Claim
27 words
A method for producing a recombinant DNA molecule comprising the step of introducing into a cloning vehicle a DNA sequence according to any one of claims 1-7.
Claim 27
Dependent Claim
47 words
The method according to claim 25 comprising the additional step of introducing into said cloning vehicle an expression control sequence according to claim 10, said expression control sequence being introduced into said cloning vehicle so as to control and to regulate the expression of said DNA sequence.
Claim 28
Independent Claim
27 words
A method for transforming a host comprising the step of introducing into a host a recombinant DNA molecule according to any one of claims 8 to 11.
Claim 29
Dependent Claim
42 words
A method for producing a polypeptide displaying an immunological or biological activity of human leukocyte interferon, comprising the steps of transforming an appropriate host with a recombinant DNA molecule according to claim 10 or 11; culturing said host; and collecting said polypeptide.
Claim 30
Dependent Claim
37 words
The method according to claim 28, wherein the host is selected from the group consisting of strains of E. coli, Pseudomonas, Bacillus subtilis, Bacillus stearothermophilus, other bacilli, yeasts, fungi, animal or plant hosts, and human tissue cells.
Claim 31
Dependent Claim
39 words
A method for producing a polypeptide displaying an immunological or biological activity of human leukocyte interferon comprising the steps of culturing a host transformed by a recombinant DNA molecule according to claim 10 or 11 and collecting said polypeptide.
Claim 32
Dependent Claim
47 words
A process for selecting a DNA sequence coding for a polypeptide displaying an immunological or biological activity of HuIFNa from a group of DNA sequences comprising the step of determining which of said DNA sequences hybridize to the DNA sequences according to any one of claims 1-7.
Claim 33
Dependent Claim
45 words
The process of claim 31 wherein the DNA sequence screened is selected from the group consisting of DNA sequences from natural sources, synthetic DNA sequences, DNA sequences from recombinant DNA molecules and DNA sequences which are a combination of any of the foregoing DNA sequences.
Claim 34
Dependent Claim
35 words
A composition for treating human viruses or treating human cancers or tumors which comprises at least one polypeptide selected from the group consisting of the polypeptides according to any one of claims 19 to 24.
Claim 35
Dependent Claim
30 words
A composition for treating bovine viral infections which comprises at least one polypeptide selected from the group consisting of the polypeptides according to any one of claims 19 to 24.
Claim 36
Dependent Claim
33 words
A method for treating human viruses or treating human cancers or tumors which comprises administering to said humans in a pharmaceutically acceptable manner an effective amount of a composition according to claim 33.
Claim 37
Dependent Claim
28 words
A method for treating bovine viral infections which comprises administering to said animals in a pharmaceutically acceptable manner an effective amount of a composition according to claim 34.
References Cited (Backward Citations)
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