Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
59 words
- 1. A composition which is a DNA sequence of at least 10 base pairs defining an autonomously replicating segment (ars) of a eukaryote, with the proviso that when said eukaryote is yeast, said autonomously replicating segment is not more than about a 1kb pair segment and free of any functional structural gene naturally linked to said ars gene.
Claim 2
Dependent Claim
14 words
A composition according to Claim 1, wherein said eukaryote is other than a yeast.
Claim 3
Dependent Claim
109 words
3 . An autonomously replicating segment (ars) from a eukaryotic host other than yeast prepared by: (1) fragmenting at least a portion of host eukaryote chromosome containing an ars to provide first DN A segments; (2) joining said first DNA segments to second DNA segments, having a gene capable of expressing a phenotypic property in yeast, to provide hybrid DNAs; (3) transforming yeast cells with said hybrid DNAs to generate yeast transformants and growing said transformants under selective conditions for said phenotypes to provide a selective transformant culture; (4) isolating hybrid DNA from said transformant culture, and (5) segmenting said hybrid DNAs to provide DNA fragments containing said ars.
Claim 4
Dependent Claim
29 words
4 . An ars according to Claim 3 , wherein said fragmenting is under conditions to generate ars containing DNA fragments substantially free of functionsl naturally present structural genes.
Claim 5
Dependent Claim
51 words
A eukaryotic cell containing hybrid DNA containing an ars endogenous to said eukaryotic cell, with the proviso that when said eukaryotic cell is a yeast cell, said ars is linked to other than structural genes naturally linked to said ars in vivo without intervention of a functional naturally linked structured gene.
Claim 6
Independent Claim
53 words
A method for generating a phenotypic property in a eukaryotic host which comprises: transforming cells of said eukaryotic host with hybrid DNA comprising a eukaryote ars gene and a gene capable of expression in said host and expressing said phenotypic property; and growing said cells. under conditions where said phenotypic property is expressed.
Claim 7
Dependent Claim
31 words
7 . A,method for producing hybrid DNA capable of autonomous replication in a eukaryotic host which comprises: . joining an ars having complementary terminii to a DNA segment having complementary terminii.
Claim 8
Dependent Claim
72 words
8 .. A hybrid DNA comprising a eukaryotic host autonomously replicating segment (ars) and a gene capable of expression in said host and derived from a source which does not normally exchange genetic information with said host, with the proviso that when said host is a yeast, the ars is linked to other than a structural gene naturally linked to said ars gene without intervention of a functional naturally linked structural gene.
Claim 9
Dependent Claim
15 words
9 . A hybrid DNA according to Claim 8,wherein said eukaryotic host is a plant.
Claim 10
Dependent Claim
95 words
In a method for enhancing the stable integration of a specified gene, capable of expression in cells of a eukaryotic host, into a chromosome of said host, said method including the steps of 1) transforming said cells with a hybrid DNA comprising said specified gene and a DNA sequence capable of - insertion into said chromosome linked to said specified gene; and 2) growing said cells containing said hybrid DNA under conditions selective for expression of said specified gene stably integrated into said chromosome; the improvement comprising: including in said hybrid DNA an ars gene.