EP0048455A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Synthetic DNA and process therefor
Assignee: National Research Development Corp UK
•
Filing: 1981-09-17
•
Granted: —
•
Expires: 2001-09-17
Abstract
Abstract A DNA molecule comprising a nucleotide sequence substantially corresponding to all or a portion of foot and mouth disease virus RNA and in particular coding for at least one protein of foot and mouth disease virus. The DNA molecule can be inserted into a DNA cloning vehicle capable of expressing the DNA molecule, after a suitable host cell has been transformed by the cloning vehicle. The expression product can be incorporated into a vaccine for stimulating antibodies against FMDV. Methods for producing the DNA molecule, recombinant cloning vehicle and transformed host cell are described.
Inventors
- 👤 John Charles Boothroyd
- 👤 George Alan Martin Cross
- 👤 Peter Edmund Highfield
- 👤 Michael David Winther
- 👤 David John Rowlands
- 👤 Fred Brown
- 👤 Timothy John Roy Harris
- 👤 Peter Anthony Lowe
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 14:05
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/32011; C12N2770/32111; C12N2770/32122
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
26 words
A DNA molecule comprising a nucleotide sequence substantially corresponding to all or a portion of foot and mouth disease virus RNA (hereinafter referred to as FMDV).
Claim 2
Dependent Claim
26 words
A DNA molecule comprising a nucleotide sequence substantially corresponding to all or a portion of foot and mouth disease virus RNA (hereinafter referred to as FMDV).
Claim 3
Dependent Claim
21 words
A DNA molecule as claimed in claim 1 comprising a nucleotide sequence substantially coding for at least one protein of FMDV.
Claim 4
Dependent Claim
21 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for a precursor of FMDV capsid proteins.
Claim 5
Dependent Claim
18 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV protein p88.
Claim 6
Dependent Claim
27 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV proteins VP,, VP 2 , VP 3 and VP 4 .
Claim 7
Dependent Claim
29 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV proteins VP 4 , VP 2 , VP 3 and VP 1 contiguously.
Claim 8
Dependent Claim
39 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV protein VP 1 contiguous with the whole or part of any one of proteins VP 2 , VP 3 and VP 4 .
Claim 9
Dependent Claim
33 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes only for FMDV protein VP 1 alone or contiguous with the whole or part of protein VP 3 .
Claim 10
Dependent Claim
20 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV protein VP 1 .
Claim 11
Dependent Claim
19 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV protein VP 2'
Claim 12
Dependent Claim
20 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV protein VP 3 .
Claim 13
Dependent Claim
20 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for FMDV protein VP 4 .
Claim 14
Dependent Claim
34 words
A DNA molecule as claimed in claim 2 wherein the nucleotide sequence substantially codes for all or a portion of at least two FMDV proteins derived from one or more different variants of FMDV.
Claim 15
Dependent Claim
24 words
A DNA molecule as claimed in claim 13 wherein the FMDV proteins or portions thereof are VP 1 proteins from different variants of FMDV.
Claim 16
Dependent Claim
19 words
A DNA molecule as claimed in claim 9 wherein the nucleotide sequence is substantially as shown in Figure 13.
Claim 17
Dependent Claim
19 words
A DNA molecule as claimed in claim 10 wherein the nucleotide sequence is substantially as shown in Figure 13.
Claim 18
Dependent Claim
19 words
A DNA molecule as claimed in claim 11 wherein the nucleotide sequence is substantially as shown in Figure 13.
Claim 19
Dependent Claim
19 words
A DNA molecule as claimed in claim 12 wherein the nucleotide sequence is substantially as shown in Figure 13.
Claim 20
Dependent Claim
23 words
A DNA molecule as claimed in any one of claims 1 to 18 wherein the nucleotide sequence codes for a biologically functional fragment.
Claim 21
Dependent Claim
32 words
A DNA molecule as claimed in any one of claims 1 to 19 wherein the nucleotide sequence corresponds to RNA or codes for a protein of FMDV serotype A or serotype O.
Claim 22
Dependent Claim
25 words
A DNA molecule as claimed in claim 20 wherein the nucleotide sequence codes for a protein of FMDV serotype A, sub-type A 10 strain 61.
Claim 23
Dependent Claim
49 words
A recombinant DNA molecule comprising an operon having initiator sequences, terminator sequences, as hereinbefore defined, and a nucleotide sequence substantially coding for all or a part of at least one protein of FMDV, the nucleotide sequence being located between the initator sequences and the terminator sequences of the operon.
Claim 24
Dependent Claim
49 words
A recombinant DNA molecule comprising an operon having initiator sequences, terminator sequences, as hereinbefore defined, and a nucleotide sequence substantially coding for all or a part of at least one protein of FMDV, the nucleotide sequence being located between the initator sequences and the terminator sequences of the operon.
Claim 25
Dependent Claim
27 words
A recombinant DNA molecule as claimed in claim 22 wherein the nucleotide sequence comprises a DNA molecule as claimed in any one of claims 2 to 21.
Claim 26
Dependent Claim
29 words
A recombinant DNA cloning vehicle capable of expressing all or part of a protein of FMDV, comprising a recombinant DNA molecule as claimed in claim 22 or claim 23.
Claim 27
Dependent Claim
15 words
A host cell containing at least one recombinant cloning vehicle as claimed in claim 24.
Claim 28
Dependent Claim
28 words
An antigen produced by a host cell transformed with recombinant DNA cloning vehicle according to claim 24 for stimulating the production of antibodies against FMDV in a mammal.
Claim 29
Dependent Claim
41 words
L. / . A vaccine for stimulating the production of antibodies against FMDV in a mammal comprising at least one protein displaying FMDV immunogenicity produced by a host cell as claimed in claim 25 together with a veterinarily acceptable carrier therefor.
Claim 30
Independent Claim
55 words
A method of preparing a DNA molecule as claimed in any one of claims 1 to 21 comprising: (a) isolating FMDV single stranded RNA; (b) preparing a first single strand of DNA complementary to the single stranded RNA; (c) preparing a second strand of DNA complementary to and hydrogen bonded to the first DNA strand.
Claim 31
Dependent Claim
67 words
A method of preparing a recombinant DNA cloning vehicle as claimed in claim 24 comprising: (a) cleaving a cloning vehicle to produce free ends; (b) inserting the DNA molecule as prepared in claim 28 in a location intermediate but not necessarily adjacent to the free ends of the cloning vehicle; (c) joining the DNA molecule to the ends of the cloning vehicle by methods known per se.
Claim 32
Dependent Claim
27 words
A method of preparing a host cell as claimed in claim 25comprising transforming a host cell with a recombinant DNA cloning vehicle as prepared in claim 29.
Claim 33
Independent Claim
60 words
A method of mapping the recombinant DNA molecule as claimed in any one of claims 1 to 21 comprising:- (a) digesting the DNA with restriction endonuclease enzymes and separating the products; (b) adding labelled oligonucleotides of FMDV RNA produced by T 1 ribonuclease digestion; (c) identifying the products of restriction endonuclease digestion to which the T 1 oligonucleotides have hybridized.
Claim 34
Dependent Claim
22 words
A DNA molecule as defined in any one of claims 1 to 21 prepared by the method as claimed in claim 28.
Claim 35
Dependent Claim
19 words
A recombinant DNA cloning vehicle as defined in claim 24 prepared by the method as claimed in claim 29.
Claim 36
Dependent Claim
17 words
A host cell as defined in claim 25 prepared by the method as claimed in claim 30.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.