EP0078636B1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Sensor for components of a liquid mixture
Assignee: Medisense Inc
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Filing: 1982-10-21
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Granted: 1988-04-13
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Expires: 2002-10-21
Abstract
No abstract record available.
Inventors
- 👤 Irving John Higgins
- 👤 Hugh Allen Oliver Hill
- 👤 Elliot Verne Plotkin
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 19:53
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
123 words
A sensor electrode (13, 14, 15) for use in detecting the presence of, measuring the amount of, and/or monitoring the level of, one or more components in a liquid mixture, by monitoring the flow of current at the electrode when an oxidoreductase enzyme catalyses a redox reaction of a substrate and a mediator compound transfers electrons between the electrode and the catalytically active oxidoreductase enzyme, the electrode being characterised in that it comprises the mediator compound and the oxidoreductase enzyme at least at an external surface (15) of electrically conductive material of the electrode the material being suitable for carrying the said flow of current, and the electrode being further characterised in that the mediator compound is a ferrocene or a ferrocene derivative.
Claim 2
Dependent Claim
123 words
A sensor electrode (13, 14, 15) for use in detecting the presence of, measuring the amount of, and/or monitoring the level of, one or more components in a liquid mixture, by monitoring the flow of current at the electrode when an oxidoreductase enzyme catalyses a redox reaction of a substrate and a mediator compound transfers electrons between the electrode and the catalytically active oxidoreductase enzyme, the electrode being characterised in that it comprises the mediator compound and the oxidoreductase enzyme at least at an external surface (15) of electrically conductive material of the electrode the material being suitable for carrying the said flow of current, and the electrode being further characterised in that the mediator compound is a ferrocene or a ferrocene derivative.
Claim 3
Dependent Claim
25 words
A sensor electrode as claimed in claim 1 characterised in that the enzyme catalyses a reaction of glucose whereby there is provided a glucose sensor.
Claim 4
Dependent Claim
17 words
A sensor electrode as claimed in claim 2, characterised in that the enzyme is a glucose oxidase.
Claim 5
Dependent Claim
18 words
A sensor electrode as claimed in claim 2, characterised in that the enzyme is a bacterial glucose dehydrogenase.
Claim 6
Dependent Claim
19 words
A sensor electrode as claimed in claim 4, characterised in that the glucose dehydrogenase is separated, from Acinetobacter calcoaceticus.
Claim 7
Dependent Claim
25 words
A sensor electrode as claimed in any of claims 1 to 5 characterised in that the mediator is ferrocene itself, 1,1'-ferrocene-dicarboxylic acid, or dimethyl ferrocene.
Claim 8
Dependent Claim
30 words
A sensor as claimed in any of claims 1 to 6 characterised in that the said electrically conductive material of the electrode is a carbon particle paste or solid carbon.
Claim 9
Dependent Claim
57 words
A sensor electrode as claimed in any of claims 1 to 7 characterised by the particular combination in which the electrically conductive material is carbon (13), the mediator compound is a layer of ferrocene or a ferrocene derivative and the enzyme is a glucose oxidase or bacterial glucose dehydrogenase located upon the layer of mediator compound (15).
Claim 10
Dependent Claim
29 words
A sensor electrode as claimed in claim 8 characterised in that the ferrocene or ferrocene derivative is in monomeric form and deposited from a readily evaporable organic solvent therefor.
Claim 11
Dependent Claim
26 words
A sensor electrode as claimed in any of claims 8 to 1 characterised by having an outermost protective membrane (11) permeable to water and glucose molecules.
Claim 12
Dependent Claim
29 words
A sensor electrode as claimed in any one of the preceding claims 1 to 10 in combination with temporary or permanent implantation means such as a needle-like probe (16).
Claim 13
Dependent Claim
17 words
A sensor electrode combination as claimed in claim 11 in further combination with signal or control equipment.
Claim 14
Independent Claim
118 words
A method of assay, excluding assay methods of treatment or diagnosis performed on the human or animal body, for detecting the . presence of, measuring the amount of, and/or monitoring the level of, one or more components in a liquid mixture, wherein a redox reaction of a substrate is catalysed by an oxidoreductase enzyme forming part of an electrode at an external surface of electrically conductive material of the electrode, a ferrocene or ferrocene derivative as mediator compound is employed at the surface of the electrode forming a part thereof to transfer electrons between the oxidoreductase enzyme and the electrode during the redox reaction, and the current generated by the transfer of electrons is monitored using the electrode.
Claim 15
Independent Claim
118 words
A method of assay, excluding assay methods of treatment or diagnosis performed on the human or animal body, for detecting the . presence of, measuring the amount of, and/or monitoring the level of, one or more components in a liquid mixture, wherein a redox reaction of a substrate is catalysed by an oxidoreductase enzyme forming part of an electrode at an external surface of electrically conductive material of the electrode, a ferrocene or ferrocene derivative as mediator compound is employed at the surface of the electrode forming a part thereof to transfer electrons between the oxidoreductase enzyme and the electrode during the redox reaction, and the current generated by the transfer of electrons is monitored using the electrode.
Claim 16
Dependent Claim
103 words
The use of a ferrocene or ferrocene derivative as a mediator compound in an assay, exclud- . ing assay methods of treatment or diagnosis performed on the human or animal body, in a liquid system by the steps of catalysing a redox reaction of a substrate with an oxidoreductase enzyme forming part of an electrode at an external surface of electrically conductive material of the electrode, employing a mediator compound at the surface of the electrode forming a part thereof to transfer electrons between the oxidoreductase enzyme and the electrode during the redox reaction, and monitoring the flow of current at the electrode.
Claim 17
Dependent Claim
103 words
The use of a ferrocene or ferrocene derivative as a mediator compound in an assay, exclud- . ing assay methods of treatment or diagnosis performed on the human or animal body, in a liquid system by the steps of catalysing a redox reaction of a substrate with an oxidoreductase enzyme forming part of an electrode at an external surface of electrically conductive material of the electrode, employing a mediator compound at the surface of the electrode forming a part thereof to transfer electrons between the oxidoreductase enzyme and the electrode during the redox reaction, and monitoring the flow of current at the electrode.
Claim 18
Dependent Claim
89 words
A ferrocene or ferrocene derivative, for use as a mediator compound in an in vivo diagnostic assay in a liquid system by the steps of catalysing a redox reaction of a substrate with an oxidoreductase enzyme forming part of an electrode at an external surface of electrically conductive material of the electrode, employing the mediator compound also forming part of the electrode at the electrode surface to transfer electrons between the oxidoreductase enzyme and the electrode during the redox reaction, and monitoring the flow of current at the electrode.
Claim 19
Dependent Claim
89 words
A ferrocene or ferrocene derivative, for use as a mediator compound in an in vivo diagnostic assay in a liquid system by the steps of catalysing a redox reaction of a substrate with an oxidoreductase enzyme forming part of an electrode at an external surface of electrically conductive material of the electrode, employing the mediator compound also forming part of the electrode at the electrode surface to transfer electrons between the oxidoreductase enzyme and the electrode during the redox reaction, and monitoring the flow of current at the electrode.
References Cited (Backward Citations)
No citation records indexed.
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