EP0085348A1
Expired / Lapsed
EP
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Reagents for the determination of enzymes
Assignee: CNA Holdings LLC
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Filing: 1983-01-18
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Granted: —
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Expires: 2003-01-18
Abstract
Abstract Assay reagents for the determination of enrymes such as α-amylase, α-glucosidase, β-glucosidase, and acid and alkaline phosphatases are described. The reagents comprise a substrate capable of releasing the chromophore p-nitrophenol at a rate proportional to the amount of enzyme being assayed and a buffer material that eliminates the temperature dependence of the chromophore.
Inventors
- 👤 Ralph Power Mccroskey
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 18:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/42; C12Q1/40; C12Q2334/00; C12Q2334/10; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/924; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
64 words
A reagent for the determination of an enzyme in a sample containing same, comprising: a substrate capable of releasing p-nitrophenol at a rate proportional to the amount of enzyme in the sample; and a buffer having a ΔpK a /°C of from about -0.010 to about -0.031 and a pKa in the range of 5 to 10 at 20°C, and mixtures of such buffers.
Claim 2
Dependent Claim
64 words
A reagent for the determination of an enzyme in a sample containing same, comprising: a substrate capable of releasing p-nitrophenol at a rate proportional to the amount of enzyme in the sample; and a buffer having a ΔpK a /°C of from about -0.010 to about -0.031 and a pKa in the range of 5 to 10 at 20°C, and mixtures of such buffers.
Claim 3
Dependent Claim
34 words
A reagent according to Claim 1 wherein said enzyme is α-amylase and said substrate is selected from the group represented by the formula where n is O to 5, and mixtures of such substrates.
Claim 4
Dependent Claim
10 words
A reagent according to Claim 2 further comprising a maltase.
Claim 5
Dependent Claim
11 words
A reagent according to Claim 3 wherein the maltase is a-glucosidase.
Claim 6
Dependent Claim
26 words
A reagent according to Claims 2, 3 or 4 wherein the buffer is selected from the group consisting of tris (hydroxymethyl) aminomethane,N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic aoid, glycylglycine and mixtures
Claim 7
Dependent Claim
26 words
A reagent according to Claim 1 wherein the enzyme is a-glucosidase and the substrate is selected from the group represented by the formulas and mixtures thereof.
Claim 8
Dependent Claim
26 words
A reagent according to Claim 1 wherein the enzyme is β-glucosidase and the substrate is selected from the group represented by the formulas and mixtures thereof.
Claim 9
Dependent Claim
24 words
A reagent according to Claims 7 or 8 wherein the buffer is selected from the group consisting of tris(hydroxymethyl)aminomethane,N-2-hydroxyethylpiperazie-N'-2-ethanesulfonic acid, glycylglycine and mixtures thereof.
Claim 10
Dependent Claim
17 words
A reagent according to Claim 1 wherein the enzyme is alkaline phosphatase and the substrate is p-nitrophenylphosphate.
Claim 11
Dependent Claim
17 words
A reagent according to Claim 1 wherein the enzyme is acid phosphatase and the substrate is p-nitrophenylphosphate.
References Cited (Backward Citations)
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