EP0092280A1
Expired / Lapsed
EP
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Semi-synthetic preparation of human insulin
Assignee: Akzo NV
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Filing: 1983-04-14
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Granted: —
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Expires: 2003-04-14
Abstract
Abstract Human insulin is semi-synthetically prepared using a novel method comprising the preparation of a modified human insulin of the general formula wherein R = H or an enzymatically separable sugar residue or an ester thereof, and R 1 = an enzymatically separable sugar residue or an ester thereof, or an amino acid or peptide, or an amide or ester of the latter two, or R 1 = OH if R ≠ H, followed by enzymatic removal of the modifying groups R and/or R 1 to yield human insulin.
Inventors
- 👤 Gijsbert Willem Karel Van Dedem
- 👤 François Egbert Abraham van Houdenhoven
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 15:58
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/26
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
95 words
Method for the semi-synthetic preparation of human insulin characterized in that from a modified human insulin of the general formula wherein R = H or an enzymatically separable sugar residue or an ester thereof, and R 1 = an enzymatically separable sugar residue or an ester thereof, or an amino acid or peptide, or an amide or ester of the latter two, or R 1 = OH if R≠H the sugar and/or amino acid or peptide as defined by R and/or R 1 are enzymatically separated, after which human insulin is isolated from the medium.
Claim 2
Dependent Claim
95 words
Method for the semi-synthetic preparation of human insulin characterized in that from a modified human insulin of the general formula wherein R = H or an enzymatically separable sugar residue or an ester thereof, and R 1 = an enzymatically separable sugar residue or an ester thereof, or an amino acid or peptide, or an amide or ester of the latter two, or R 1 = OH if R≠H the sugar and/or amino acid or peptide as defined by R and/or R 1 are enzymatically separated, after which human insulin is isolated from the medium.
Claim 3
Dependent Claim
60 words
Method for the semi-synthetic preparation of human insulin according to claim 1 comprising bonding to des(B30)insulin or a des(B30)insulin residue the compound wherein R and R 1 have the meaning defined in claim 1, enzymatic separation of the sugar and/or amino acid or peptide as defined by R and/or R l , and isolation of human insulin from the medium.
Claim 4
Dependent Claim
56 words
Method for the semi-synthetic preparation of human insulin according to claim 1 comprising bonding to des(B29,B30)insulin the compound wherein R and R 1 have the meaning defined in claim 1, enzymatic separation of the sugar and/or amino acid or peptide as defined by R and/or R l , and. isolation of human insulin from the medium.
Claim 5
Dependent Claim
29 words
Method as claimed in claim 1-3, characterized in that the group R= H and R 1 = an amino acid, dipeptide or tripeptide or an ester or amide thereof.
Claim 6
Dependent Claim
21 words
Method as claimed in claim 4, characterized in that the group R 1 = a mono-, di- or trimer of phenylalanine.
Claim 7
Dependent Claim
18 words
Method as claimed in claim 5, characterized in that the group R 1 is enzymatically separated using carboxypeptidase.
Claim 8
Dependent Claim
14 words
Method as claimed in claim 6, characterized in that the carboxypeptidase is carboxypeptidase A.
Claim 9
Dependent Claim
20 words
Modified human insulin characterized by the formula wherein R and R 1 have the meaning as defined in claim 1.
References Cited (Backward Citations)
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