EP0100217A2
Expired / Lapsed
EP
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Process for quantitative determination of substrate treated with oxidase
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1983-07-22
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Granted: —
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Expires: 2003-07-22
Abstract
Abstract A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent. Further, the peroxidase can be replaced by (b') iron complex of porphyrin or ion chelate of complexane.
Inventors
- 👤 Yoshitsugu Sakata
- 👤 Yoshinobu Miyashita
- 👤 Tadashi Hamanaka
- 👤 Hiroyuki Kodera
- 👤 Yutaka Miki
- 👤 Kazuhiko Yamanishi
- 👤 Toshiro Hanada
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 02:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/62; C12Q1/26; C12Q1/54; C12Q1/60
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
29 words
A process for quantitatively determining a substrate, which comprises treating a sample containing a substrate with a specific oxidase corresponding to said substrate, and measuring the generated superoxide ion.
Claim 2
Dependent Claim
29 words
A process for quantitatively determining a substrate, which comprises treating a sample containing a substrate with a specific oxidase corresponding to said substrate, and measuring the generated superoxide ion.
Claim 3
Dependent Claim
38 words
A process according to Claim 1, wherein the measuring of the genrated superoxide ion is conducted by measuring color formation or color change produced by reduction of a color producing reagent to be reduced by the superoxide ion.
Claim 4
Dependent Claim
83 words
A process according to Claim 1, wherein the substrate is selected from the group consisting of glucose, cholesterol, glycerol, glycerol phosphate, choline, acyl CoA, pyruvic acid, uric acid, xanthine and lactic acid and said oxidase treatment and measuring of generated superoxide ion conducted by measuring color formation or color change is conducted by using a reagent composition comprising (a) an oxidase, (b) a peroxidase, (c) a phenol and/or an amine (d) a thiol compound and (e) a color producing reagent to be reduced.
Claim 5
Dependent Claim
87 words
A process according to Claim 1, wherein the substrate is selected from the group consisting of glucose, cholesterol, glycerol, glycerol phosphate, choline, acyl CoA, pyruvic acid, uric acid, xanthine and lactic acid and said oxidase treatment and measuring of generated superoxide ion conducted by measuring color formation or color change is conducted by using a reagent composition comprising (a) an oxidase, (b) a peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and (f) a chelating agent.
Claim 6
Dependent Claim
100 words
A process according to Claim 1, wherein the substrate is one member selected from the group consisting of glucose, cholesterol, glycerol, glycerol phosphate, choline, acyl CoA, pyruvic acid, uric acid, xanthine and lactic acid, and said oxidase treatment and measuring of generated superoxide ion conducted by measuring color formation or color change is conducted by using a reagent composition comprising (a) a specific oxidase corresponding to a substrate to be determined, (b') iron complex of porphyrin or iron chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound, and (e) a color producing reagent to be reduced.
Claim 7
Dependent Claim
103 words
A process according to Claim 1, wherein the substrate is one member selected from the group consisting of glucose, cholesterol, glycerol, glycerol phosphate, choline, acyl CoA, pyruvic acid, uric acid, xanthine and lactic acid, and said oxidase treatment and'measuring of generated superoxide ion conducted by measuring color formation or color change is conducted by using a reagent composition comprising (a) a specific oxidase corresponding to a substrate to be determined, (b') iron complex of porphyrin or iron chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound, (e) a color producing reagent to be reduced, and (f) a chelating agent.
Claim 8
Dependent Claim
42 words
A composition for quantitative determination of a substrate comprising (a) a specific oxidase corresponding to a substrate to be determined, (b) a peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, and (e) a color producing reagent to be reduced.
Claim 9
Dependent Claim
42 words
A composition for quantitative determination of a substrate comprising (a) a specific oxidase corresponding to a substrate to be determined, (b) a peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, and (e) a color producing reagent to be reduced.
Claim 10
Dependent Claim
13 words
A composition according to Claim 7, which further comprises (f) a chelating agent.
Claim 11
Dependent Claim
49 words
A composition for quantitative determination of a substrate comprising (a) a specific oxidase corresponding to a substrate to be determined, (b') iron complex of porphyrin or iron chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound and (e) a color producing reagent to be reduced.
Claim 12
Dependent Claim
49 words
A composition for quantitative determination of a substrate comprising (a) a specific oxidase corresponding to a substrate to be determined, (b') iron complex of porphyrin or iron chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound and (e) a color producing reagent to be reduced.
Claim 13
Dependent Claim
13 words
A composition according to Claim 9, which further comprises (f) a chelating agent.
References Cited (Backward Citations)
No citation records indexed.
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