EP0106615A2
Expired / Lapsed
EP
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Assay for the free portion of substances in biological fluids
Assignee: GE Healthcare Ltd
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Filing: 1983-10-03
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Granted: —
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Expires: 2003-10-03
Abstract
Abstract @ An assay for the free portion of an analyte, present in a biological fluid which also contains the analyte bound to natural binders, comprises incubating a sample of the fluid with a specific binder for the analyte and an antibody to the specific binder whereby the analyte and the antibody are caused to compete for binding sites on the specific binder. The antibody is preferably an anti-idiotype antibody to the specific binder. One of the specific binder and the antibody is labelled, whereby the proportion of the labelled reagent bound to its binding partner can be measured and used to determine the free analvte concentration in the samole.
Inventors
- 👤 Anthony John Brockas
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 04:09
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/577; G01N33/543; G01N33/54306; G01N33/68; G01N33/6854; G01N33/686
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
219 words
A method of determining the concentration of the free portion of an analyte which is a member of a specific binding pair consisting of the analyte and a specific binder therefor, said free portion of the analyte being present in a biological fluid which also contains a portion of the analyte bound to one or more natural binders for the analyte, the bound and free portions of the analyte being in equilibrium with one another, by:- a) forming a mixture of a sample of the biological fluid with an amount of the specific binder for the analyte and with an antibody to the specific binder, the amount of said specific binder being insufficient to substantially affect said equilibrium, at least one of said specific binder and said antibody being labelled; b) maintaining said mixture for a time to permit the free portion of the analyte and the antibody to compete for binding sites on the specific binder and become bound thereto in proportions which depend on the concentration of the free portion of the analyte present in the sample; e) measuring the amount of the specific binder bound to the antibody, and/or the amount of the specific binder not bound to the antibody; and d) using the measurement to determine the concentration of free analyte in the biological fluid.
Claim 2
Independent Claim
219 words
A method of determining the concentration of the free portion of an analyte which is a member of a specific binding pair consisting of the analyte and a specific binder therefor, said free portion of the analyte being present in a biological fluid which also contains a portion of the analyte bound to one or more natural binders for the analyte, the bound and free portions of the analyte being in equilibrium with one another, by:- a) forming a mixture of a sample of the biological fluid with an amount of the specific binder for the analyte and with an antibody to the specific binder, the amount of said specific binder being insufficient to substantially affect said equilibrium, at least one of said specific binder and said antibody being labelled; b) maintaining said mixture for a time to permit the free portion of the analyte and the antibody to compete for binding sites on the specific binder and become bound thereto in proportions which depend on the concentration of the free portion of the analyte present in the sample; e) measuring the amount of the specific binder bound to the antibody, and/or the amount of the specific binder not bound to the antibody; and d) using the measurement to determine the concentration of free analyte in the biological fluid.
Claim 3
Dependent Claim
14 words
A method as claimed in claim 1, wherein the antibody is an anti-idiotype antibody.
Claim 4
Dependent Claim
15 words
A method as claimed in claim 1 or claim 2, wherein the antibody is labelled.
Claim 5
Dependent Claim
17 words
A method as claimed in claim 3, wherein the specific binder is used in an insoluble form.
Claim 6
Independent Claim
22 words
A method as claimed in any one of claims 1 to 3, wherein the specific binder is an antibody to the analyte.
Claim 7
Dependent Claim
15 words
A method as claimed in claim 5, wherein the specific binder is a monoclonal antibody.
Claim 8
Independent Claim
23 words
A method as claimed in any one of claims 2 to 6, wherein the antibody is a monoclonal antibody to the specific binder.
Claim 9
Dependent Claim
96 words
An assay kit for use in the method of any one of claims 1 to 7, for determining the concentration of the free portion of an analyte present in a biological fluid which also contains a portion of the analyte bound to one or more natural binders for the analyte, the bound and free portions of the analyte being in equilibrium with one another, comprising a supply of a specific binder for the analyte, and a supply of an antibody to the specific binder, at least one of the specific binder and the antibody being labelled.
Claim 10
Dependent Claim
21 words
A kit as claimed in claim 8, wherein the specific binder is in an insoluble form and the antibody is labelled.
Claim 11
Dependent Claim
33 words
A kit as claimed in claim 8 or claim 9, wherein the specific binder is a monclonal antibody to the analyte and the antibody is a monclonal anti-idiotype antibody to the specific binder.
References Cited (Backward Citations)
No citation records indexed.
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