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EP0112012A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Recombinant DNA molecules

Assignee: Case Western Reserve University
•
Filing: 1983-11-01
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Granted: —
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Expires: 2003-11-01
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Abstract

Abstract A bovine growth hormone genomic clone has been prepared by use of recombinant DNA techniques. The nucleotide sequence of this genomic clone, and functional equivalents thereof, can be cloned into suitable vectors for the transformation into micro-organism hosts which can then be cultured to produce bovine growth hormone. Novel modified hosts and plasmids are described.

Inventors

  • 👤 Fritz Max Rottman
  • 👤 Richard P. Woychik
  • 👤 John Helmer Nilson

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-24 05:33
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61

Claims Analysis

Total Claims: 15
Claim 1
Independent Claim 10 words
The nucleotide sequence of genomic bovine growth hormone as follows:
Claim 2
Dependent Claim 10 words
The nucleotide sequence of genomic bovine growth hormone as follows:
Claim 3
Dependent Claim 17 words
A DNA transfer vector comprising the nucleotide sequence identified in claim 1 or a functional equivalent thereof.
Claim 4
Dependent Claim 17 words
A host modified to contain the nucleotide sequence identified in claim 1 or a functional equivalent thereof.
Claim 5
Dependent Claim 20 words
A host according to claim 3, which is a micro-organism containing a replicatable DNA transfer vector according to claim 2.
Claim 6
Dependent Claim 26 words
A host according to claim 4, in which the micro-organism is Escherichia coli X 1776 or Escherichia coli HB101, and in which the plasmid is pBR322.
Claim 7
Dependent Claim 20 words
A process for preparing bovine growth hormone, which comprises culturing a host according to any of claims 3 to 5.
Claim 8
Dependent Claim 2 words
Plasmid pXGH2R2.
Claim 9
Dependent Claim 2 words
Plasmid pXGH2R2.
Claim 10
Dependent Claim 2 words
Plasmid pλGH2R3.
Claim 11
Dependent Claim 2 words
Plasmid pλGH2R3.
Claim 12
Dependent Claim 6 words
E. coli HB101 (pXGH2R2), NRRL B-15154.
Claim 13
Dependent Claim 6 words
E. coli HB101 (pXGH2R2), NRRL B-15154.
Claim 14
Dependent Claim 6 words
E. coli HB101 (pXGH2R3), NRRL B-15155.
Claim 15
Dependent Claim 6 words
E. coli HB101 (pXGH2R3), NRRL B-15155.

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