EP0123115A2
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EP
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Ascorbate interference-resistant composition, device and method for the determination of peroxidatively active substances
Assignee: Bayer Corp
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Filing: 1984-03-16
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Granted: —
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Expires: 2004-03-16
Abstract
Abstract A composition, test means (and device) and method for determining peroxidatively activesubstances in a testsample are disclosed. The composition, test means (and device) and method are rendered resistant to the adverse affects of ascorbate which may be present in the sample by the inclusion in the composition of a metal chelate which is polycarboxyalkylamine derivative having the formula: where: (a) R, is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; R 2 , R 3 , Rx and Ry, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; where at least two of R 1 , R 2 , Rs, Rx or Ry are alkyl carboxylic acid radicals so defined; (b) Rp and Rq, same or different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms; (c) n is an integer having a value of from 0 to 1; m is an integer having a value of from Oto 2; where ifm is greaterthan 0, repeated Rp and repeated Rq radicals may be the same or different; and (d) M is Fe +3 . The composition also comprises an organic hydroperoxide and an indicator capable of providing a detectable response in the presence of peroxide and the peroxidatively active substance. The test means comprises a carrier matrix incorporated with the composition, and the method comprises immersing the test means (or device) in the test sample and observing a color or other detectable response.
Inventors
- 👤 Christine Mayambala-Mwanika
- 👤 Rodric H. White-Stevens
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 08:19
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
229 words
A composition for detecting the presence of a peroxidatively active substance in a test sample, the composition comprising an organic hydroperoxide and an indicator capable of providing a detectable response in the presence of the peroxidatively active substance and peroxide, characterized in that the composition addi- - tionally comprises a meta] chelate of a polycarboxyalkylamine derivative having the general formula: where: (a) R1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; R 2 , R 3 , R x and R y , same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; where at least two of R 1 , R 2 , R 3 , R x or R y are alkyl carboxylic acid radicals so defined; (b) R and R q , same or different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms; (c) n is an integer having a value of from 0 to 1; m is an integer having a value of from 0 to 2; where if m is greater than 0, repeated R p and repeated R radicals may be the same or different; and (d) M is Fe .
Claim 2
Dependent Claim
229 words
A composition for detecting the presence of a peroxidatively active substance in a test sample, the composition comprising an organic hydroperoxide and an indicator capable of providing a detectable response in the presence of the peroxidatively active substance and peroxide, characterized in that the composition addi- - tionally comprises a meta] chelate of a polycarboxyalkylamine derivative having the general formula: where: (a) R1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; R 2 , R 3 , R x and R y , same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; where at least two of R 1 , R 2 , R 3 , R x or R y are alkyl carboxylic acid radicals so defined; (b) R and R q , same or different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms; (c) n is an integer having a value of from 0 to 1; m is an integer having a value of from 0 to 2; where if m is greater than 0, repeated R p and repeated R radicals may be the same or different; and (d) M is Fe .
Claim 3
Dependent Claim
21 words
A composition according to claim 1, characterized in that (a) m is 0; and (b) R p is an ethylene radical.
Claim 4
Dependent Claim
37 words
A composition according to claim 1 or 2, characterized in that at least two of R 1 , R 2 , R 3 , R X and Ry are the alkyl carboxylic acid radical, -CH 2 COOH.
Claim 5
Dependent Claim
42 words
A composition according to claim 1, characterized in that the metal chelate is selected from the group consisting of ferric chelates of N-(2-hydroxyethyl)-ethylenediaminetriacetic acid, ethylenediaminetetraacetic acid, cyclohexylenediaminetetraacetic acid, nitrilotriacetic acid, iminodiacetic acid, a-ethylenediaminediaceticdi- propionic acid, β-ethylenediaminediaceticdipropionic acid, hydroxylethylaminodiacetic acid and mixtures thereof.
Claim 6
Dependent Claim
24 words
A composition according to any of claims 1 to 4, characterized in that the organic hydroperoxide is cumene hydroperoxide and the indicator is 3,3',5,5'-tetramethylbenzidine.
Claim 7
Dependent Claim
36 words
A test means for determining the presence of a peroxidatively active substance in a test sample, characterized in that it comprises a carrier matrix incorporated with a composition according to any of claims 1 to 5.
Claim 8
Independent Claim
338 words
A method for preparing a test means for determining the presence of a peroxidatively active substance in a test sample, wherein the test means is resistant to interfering affects of ascorbate which may be present in the sample, which method comprises the steps of: a) preparing a first reagent solution including an organic hydroperoxide, and a metal chelate of an polycarboxyalkylamine derivative having the general formula: where: (i) R 1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; R 2 , R 3 , R x and Ry, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; where at least two of R 1 , R 2 , R 3 , R x or R y are alkyl carboxylic acid radicals so defined; (ii) R and R , same of different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms; (iii) n is an integer having a value of from 0 to 1; m is an integer having a value of from 0 to 2; where if m is greater than 0, repeated R and repeated R radicals may be the same or different; and (iv) M is Fe +3 ; b) incorporating the first reagent solution with a carrier matrix by wetting the matrix with the first solution; c) drying the wetted matrix to leave a residue of the metal chelate and the hydroperoxide; d) preparing a second reage solution including an indicator and a solvent, the indicator being capable of providing a detectable response in the presence of a peroxide and the peroxidatively active substance; e) incorporating the second reagent solution with the dried carrier matrix by wetting the matrix with the second reagent solution; and f) drying the matrix to leave a combined residue including the metal chelate, the hydroperoxide and the indicator.
Claim 9
Independent Claim
338 words
A method for preparing a test means for determining the presence of a peroxidatively active substance in a test sample, wherein the test means is resistant to interfering affects of ascorbate which may be present in the sample, which method comprises the steps of: a) preparing a first reagent solution including an organic hydroperoxide, and a metal chelate of an polycarboxyalkylamine derivative having the general formula: where: (i) R 1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; R 2 , R 3 , R x and Ry, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; where at least two of R 1 , R 2 , R 3 , R x or R y are alkyl carboxylic acid radicals so defined; (ii) R and R , same of different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms; (iii) n is an integer having a value of from 0 to 1; m is an integer having a value of from 0 to 2; where if m is greater than 0, repeated R and repeated R radicals may be the same or different; and (iv) M is Fe +3 ; b) incorporating the first reagent solution with a carrier matrix by wetting the matrix with the first solution; c) drying the wetted matrix to leave a residue of the metal chelate and the hydroperoxide; d) preparing a second reage solution including an indicator and a solvent, the indicator being capable of providing a detectable response in the presence of a peroxide and the peroxidatively active substance; e) incorporating the second reagent solution with the dried carrier matrix by wetting the matrix with the second reagent solution; and f) drying the matrix to leave a combined residue including the metal chelate, the hydroperoxide and the indicator.
Claim 10
Dependent Claim
22 words
A method according to claim 7, characterized in that the indicator is benzidine; o-tolidine; a 3,3', 5,5'-tetra(lower alkyl)benzidine; 2,7-diaminofluorene; or mixtures thereof.
Claim 11
Dependent Claim
33 words
A method according to claim 7 or 8, characterized in that the organic hydroperoxide is selected from the group consisting of cumene hydroperoxide; t-butyl hydroperoxide, diisopropylbenzene hydroperoxide, 2,5-dimethylhexane-2,5-dihydroperoxide; paramenthane hydroperoxide; and mixtures thereof.
Claim 12
Independent Claim
42 words
A method for determining the presence of a peroxidatively active substance in a test sample, characterized in that it comprises the steps of contacting the sample with a composition according to any of claims 1 to 5 and observing a detectable response.
References Cited (Backward Citations)
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