EP0125137B1
Expired / Lapsed
EP
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Measurement of enzyme-catalysed reactions
Assignee: Medisense Inc
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Filing: 1984-05-08
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Granted: 1990-08-22
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Expires: 2004-05-08
Abstract
No abstract record available.
Inventors
- 👤 Irving John Higgins
- 👤 Hugh Allen Oliver Hill
- 👤 Elliot Verne Plotkin
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 12:00
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/145; A61B5/1468; A61B5/1486; A61B5/14865; A61B5/68; A61B5/6846; A61B5/6847; A61B5/6848; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
134 words
An electrode sensor system for sensing the presence of at least one selected component of a mixture of components said sensor system comprising: at least two electrically conducting means insulated from each other, each of which is in electrical contact with said mixture via an electrically conductive surface. An enzyme which is a non-oxygen specific flavor protein other than glucose oxidase, a quino-protein other than glucose dehydrogenase, a haem-containing enzyme, a cupro-protein, or carbonmonoxide oxidoreductase, a mediator compound which transfers electrons between said enzyme and one said conductive surface when said enzyme is catalytically active, said mediator being an organometallic compound which comprises at least two organic rings, each of which is characterised by at least two double bonds that are conjugated and a metal atom in electron-sharing contact with each of said rings.
Claim 2
Dependent Claim
134 words
An electrode sensor system for sensing the presence of at least one selected component of a mixture of components said sensor system comprising: at least two electrically conducting means insulated from each other, each of which is in electrical contact with said mixture via an electrically conductive surface. An enzyme which is a non-oxygen specific flavor protein other than glucose oxidase, a quino-protein other than glucose dehydrogenase, a haem-containing enzyme, a cupro-protein, or carbonmonoxide oxidoreductase, a mediator compound which transfers electrons between said enzyme and one said conductive surface when said enzyme is catalytically active, said mediator being an organometallic compound which comprises at least two organic rings, each of which is characterised by at least two double bonds that are conjugated and a metal atom in electron-sharing contact with each of said rings.
Claim 3
Dependent Claim
15 words
The system of claim 1 wherein said mediator is confined at said electrically conducting surface.
Claim 4
Dependent Claim
15 words
The system of claim 1 wherein said enzyme is confined at said electrically conducting surface.
Claim 5
Dependent Claim
16 words
The system of claim 1 wherein said selected component is a substrate for said enzyme-catalyzed reaction.
Claim 6
Dependent Claim
13 words
The system of claim 1 wherein said mediator compound is a ferrocene-type compound.
Claim 7
Dependent Claim
30 words
The system of claim 5 wherein said ferrocene-type compound is selected from the group consisting of ferrocene; chloroferrocene; methyl-trimethylaminoferrocene; 1,1-dimethylferrocene; 1,1-dicarboxyferrocene; carboxyferrocene; vinylferrocene; trimethylferrocene; polyvinylferrocene; hydroxyethylferrocene; acetoferrocene; and 1,1'-bishydroxymethyl ferrocene.
Claim 8
Dependent Claim
29 words
The system of claim 1 wherein said enzyme is selected from the group consisting of pyruvate oxidase, xanthine oxidase, sarcosine oxidase, darbonmonoxide oxidoreductase, glycolate oxidase and L-amino acid oxidase.
Claim 9
Dependent Claim
11 words
The system of claim 1 wherein said enzyme is methanol dehydrogenase.
Claim 10
Dependent Claim
11 words
The system of claim 1 wherein said metal atom is iron.
Claim 11
Dependent Claim
11 words
The system of claim 1 wherein said metal atom is ruthenium.
Claim 12
Dependent Claim
25 words
A sensor as claimed in claim 1 in which the electrode is made of a material chosen from silver, carbon particle paste and solid carbon.
Claim 13
Dependent Claim
16 words
The sensor system of claim 1 with implantation means suitable for implantation in a human subject.
Claim 14
Dependent Claim
19 words
A sensor system of claim 1 wherein said mediator compound transfers electrons from said enzyme to said conductive surface.
Claim 15
Dependent Claim
25 words
The system of claim 1 in which the enzyme is chosen from the group consisting of lactate dehydrogenase, yeast cytochrome c peroxidase and horseradish peroxidase.
Claim 16
Dependent Claim
12 words
The system of claim 1 in which the enzyme is galactose oxidase.
Claim 17
Dependent Claim
17 words
The system of claim 1, wherein the mediator is covalently bond to the electrode and/or the enzyme.
References Cited (Backward Citations)
No citation records indexed.
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