EP0140521A2
Expired / Lapsed
EP
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Specific binding assay
Assignee: Bayer Corp
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Filing: 1984-08-24
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Granted: —
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Expires: 2004-08-24
Abstract
Abstract @ A specific binding assay composition for determining a ligand in a sample, comprises (a) a binding partner for the ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface-incorporated ligand or ligand analog and a first component of the detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-associated ligand or ligand analog and binding partner; and (e) at least one additional component of the detection system which is reactive with the first component to produce a detectable response which is reduced by association of the binding partner and vescile modifying substance with the variably accessible vesicle. A decrease in signal is measured upon reaction as compared to the signal of unreacted vesicles.
Inventors
- 👤 John P. Fox
- 👤 Eddie Hedaya
- 👤 Violet Lippman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 07:30
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S435/968; Y10S436/00; Y10S436/829
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
111 words
A composition for determining a ligand in a sample, which composition comprises: (a) a binding partner for said ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface incorporated ligand or ligand analog, and a first component of said detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-incorporated ligand or ligand analog and the binding partner; and (e) at least one additional component of said detection system which is reactive with said first component to produce a detectable response which is reduced by association of the binding partner and vesicle modifying substance with the vesicle.
Claim 2
Dependent Claim
111 words
A composition for determining a ligand in a sample, which composition comprises: (a) a binding partner for said ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface incorporated ligand or ligand analog, and a first component of said detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-incorporated ligand or ligand analog and the binding partner; and (e) at least one additional component of said detection system which is reactive with said first component to produce a detectable response which is reduced by association of the binding partner and vesicle modifying substance with the vesicle.
Claim 3
Dependent Claim
27 words
A composition according to claim 1, wherein the vesicle exhibits a detectable response in the absence of association of the binding partner and vesicle modifying substance therewith.
Claim 4
Dependent Claim
25 words
A composition according to claim 2, wherein the detectable response is reduced upon association of the binding partner and vesicle modifying substance with said vesicle.
Claim 5
Dependent Claim
35 words
A composition according to claim 1 wherein the vesicle is comprised of a lipid membrane, or a phospholipid, or incorporates a sterol, or includes an amphiphile to which the ligand or ligand analog is bound.
Claim 6
Dependent Claim
15 words
- A composition according to claim 1, wherein the vesicle is a biological cell membrane.
Claim 7
Dependent Claim
16 words
A composition according to any of claims 1 to 5, wherein the detection system is luminescent.
Claim 8
Dependent Claim
32 words
A composition according to claim 6, wherein the first component of the detection system comprises a peroxidatively active substance for example peroxidase, and the at least one additional component comprises a luminophore.
Claim 9
Dependent Claim
34 words
A composition according to claim 7, wherein the at least one additional component further comprises an oxidant, for example hydrogen peroxide, for the luminophore, which oxidant is a substrate for the peroxidatively active substance.
Claim 10
Dependent Claim
32 words
A composition according to claim 7, wherein the at least one additional component further comprises an enzymatic system, for example glucose oxidase and glucose, effective to produce an oxidant for the luminophore.
Claim 11
Dependent Claim
20 words
A composition according to any of claims 1 to 9, wherein the detection system further comprises an energy transfer system.
Claim 12
Dependent Claim
38 words
A composition according to claims 9 and 10, wherein the detection system comprises a luminophore and the energy transfer system comprises fluor which absorbs light energy at the wavelength of the luminophore and fluoresces at a different wavelength.
Claim 13
Dependent Claim
31 words
A composition according to claim 6, which comprises an additional substance such as an inhibitor, for example cyanide, which diminishes the luminescence of said detection system upon variation of vesicle accessibility.
Claim 14
Dependent Claim
26 words
A composition according to any of claims 1 to 12, wherein the ligand is a hapten or antigen, and the binding partner is an antibody therefor.
Claim 15
Dependent Claim
17 words
A composition according to any of claims 1 to 12, wherein the vesicle modifying substance is complement.
Claim 16
Dependent Claim
59 words
A composition for determining a ligand in a sample, which composition comprises: (a) antibody for said ligand; (b) complement; (c) a luminophore; (d) hydrogen peroxide; and (e) a liposome having a ligand or ligand analog on the surface thereof and peroxidase therein, which liposome exhibits a detectable signal that is reduced upon being bound with said antibody and complement.
Claim 17
Dependent Claim
59 words
A composition for determining a ligand in a sample, which composition comprises: (a) antibody for said ligand; (b) complement; (c) a luminophore; (d) hydrogen peroxide; and (e) a liposome having a ligand or ligand analog on the surface thereof and peroxidase therein, which liposome exhibits a detectable signal that is reduced upon being bound with said antibody and complement.
Claim 18
Dependent Claim
21 words
The use of a composition as claimed in any of claims 1 to 15, for determining a ligand in a sample.
References Cited (Backward Citations)
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