EP0149853A2
Expired / Lapsed
EP
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Process for measuring activity of dehydrogenase
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1984-12-28
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Granted: —
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Expires: 2004-12-28
Abstract
Abstract In a process for measuring the activity of a dehydrogenase or the amount of substrate reacted in the presence of the dehydrogenase using NAD or NADP as coenzyme and a tetrazorium salt as a color-producing reagent, when at least one member selected from the group consisting of dodecyl sulfate, decyl sulfate, dodecylbenzenesulfonic acid, and salts thereof is used, deposition and staining of a formazan compound produced can be prevented and improvement in color-producing stability and enhancement of coloring can be attained.
Inventors
- 👤 Kazuhiko Yamanishi
- 👤 Toshiro Hanada
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 20:46
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
87 words
In a process for measuring an activity of a dehydrogenase comprising conducting an enzymatic reaction of a dehydrogenase in the presence of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate as coenzyme and a tetrazolium salt as a color-producing reagent, stopping the reaction by adding a reaction stopper thereto, and conducting colorimetrial measurement, the improvement wherein as the reaction stopper, there is used at least one member selected from the group consisting of decyl sulfate and salts thereof, dodecyl sulfate and salts thereof, dodecylbenzenesulfonic acid and salts thereof.
Claim 2
Dependent Claim
87 words
In a process for measuring an activity of a dehydrogenase comprising conducting an enzymatic reaction of a dehydrogenase in the presence of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate as coenzyme and a tetrazolium salt as a color-producing reagent, stopping the reaction by adding a reaction stopper thereto, and conducting colorimetrial measurement, the improvement wherein as the reaction stopper, there is used at least one member selected from the group consisting of decyl sulfate and salts thereof, dodecyl sulfate and salts thereof, dodecylbenzenesulfonic acid and salts thereof.
Claim 3
Dependent Claim
22 words
A process according to Claim 1, wherein the salts of decyl sulfate, dodecyl sulfate and dodecylbenzenesulfonic acid are alkali metal salts thereof.
Claim 4
Dependent Claim
15 words
A process according to Claim 1, wherein the reaction stopper is used together with gelatin.
Claim 5
Dependent Claim
14 words
A process according to Claim 1, wherein the reaction stopper is sodium dodecyl sulfate.
Claim 6
Dependent Claim
14 words
A process according to Claim 3, wherein the reaction stopper is sodium dodecyl sulfate.
Claim 7
Dependent Claim
86 words
In a process for determining the amount of a substrate comprising reacting a substrate in the presence of a dehydrogenase using nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate as coenzyme and a tetrazolium salt as a color-producing reagent, stopping the reaction by adding a reaction stopper thereto, and conducting colorimetical determination, the improvement wherein as the reaction stopper there is used at least one member selected from the group consisting of decyl sulfate and salts thereof, dodecyl sulfate and salts thereof, dodecylbenzenesulfonic acid and salts thereof.
Claim 8
Dependent Claim
86 words
In a process for determining the amount of a substrate comprising reacting a substrate in the presence of a dehydrogenase using nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate as coenzyme and a tetrazolium salt as a color-producing reagent, stopping the reaction by adding a reaction stopper thereto, and conducting colorimetical determination, the improvement wherein as the reaction stopper there is used at least one member selected from the group consisting of decyl sulfate and salts thereof, dodecyl sulfate and salts thereof, dodecylbenzenesulfonic acid and salts thereof.
Claim 9
Dependent Claim
24 words
7 . A process according to Claim 6, wherein the salts of decyl sulfate, dodecyl sulfate and dodecylbenzenesulfonic acid are alkali metal salts thereof.
Claim 10
Dependent Claim
15 words
A process according to Claim 6, wherein the reaction stopper is used together with gelatin.
Claim 11
Dependent Claim
14 words
A process according to Claim 6, wherein the reaction stopper is sodium dodecyl sulfate.
Claim 12
Dependent Claim
14 words
A process according to Claim 8, wherein the reaction stopper is sodium dodecyl sulfate.
Claim 13
Dependent Claim
35 words
Use of decyl sulfate and salts thereof, dodecyl sulfate and salts thereof and/or dodecylbenzenesulfonic acid and salts thereof in a process for measuring the activity of dehydrogenase as described in anyone of the preceeding claims.
References Cited (Backward Citations)
No citation records indexed.
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