EP0157197A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for carrying out immunofluorescent assays
Assignee: Thermo Fisher Scientific Oy
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Filing: 1985-03-05
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Granted: —
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Expires: 2005-03-05
Abstract
Abstract Method for carrying out immunoassays, in which method an antibody (or antigen) marked with a tracer so as to be fluorescent is attached onto an antigen (or antibody, respectively) present at the inside wall of the measurement vessel, a liquid denser than the sample is added to the measurement vessel, which said denser liquid displaces the liquid from underneath, and both the excitation radiation is passed into the sample and the fluorescent radiation is collected to the detector through the wall or the bottom of the measurement vessel.
Inventors
- 👤 Hannu Harjunmaa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 23:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/5375; G01N33/543; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/81; Y10S436/824; Y10S436/826
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
113 words
Method for carrying out immunoassays, in which method an antibody or antigen marked with a fluorescent or phosphorescent tracer and attached to the bottom or to the side walls of the measurement vessel is excited by means of appropriate radiation and the fluorescent or phosphorescent radiation emitted from the antibody or antigen is measured, characterized in that, before the measurement, a liquid denser than the sample is dosed into the measurement vessel, which said denser liquid displaces the liquid from underneath, and that both the excitation radiation is passed into the sample and the fluorescent or phosphorescent radiation is collected to the detectors through the wall or the bottom of the measurement vessel.
Claim 2
Dependent Claim
113 words
Method for carrying out immunoassays, in which method an antibody or antigen marked with a fluorescent or phosphorescent tracer and attached to the bottom or to the side walls of the measurement vessel is excited by means of appropriate radiation and the fluorescent or phosphorescent radiation emitted from the antibody or antigen is measured, characterized in that, before the measurement, a liquid denser than the sample is dosed into the measurement vessel, which said denser liquid displaces the liquid from underneath, and that both the excitation radiation is passed into the sample and the fluorescent or phosphorescent radiation is collected to the detectors through the wall or the bottom of the measurement vessel.
Claim 3
Dependent Claim
34 words
Method as claimed in claim 1, characterized in that a liquid denser than the sample is dosed into the measurement vessel, which said denser liquid absorbs strongly at the wavelength of the excitation radiation.
Claim 4
Dependent Claim
36 words
Method as claimed in claim 1 or 2, characterized in that a liquid denser than the sample is dosed into the measurement vessel, which said denser liquid absorbs strongly at the wavelength of the emission radiation.
Claim 5
Dependent Claim
19 words
Method as claimed in claim 2 or 3, characterized in that the liquid denser than the sample is black.
Claim 6
Dependent Claim
40 words
Method as claimed in any of claims 2 to 4, characterized in that both the excitation radiation is passed into the sample and the fluorescent or phosphorescent radiation is collected to the detector through the bottom of the measurement vessel.
Claim 7
Dependent Claim
38 words
Method as claimed in any of the claims 1 to 5, characterized in that a liquid denser than the sample is dosed into the measurement vessel, which said denser liquid is allowed to solidify in the measurement vessel.
References Cited (Backward Citations)
No citation records indexed.
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