EP0158973A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Multisite immunometric assay
Assignee: Institut Gustave Roussy
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Filing: 1985-04-11
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Granted: —
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Expires: 2005-04-11
Abstract
Abstract An immunometric assay for a multivalent antigen in a sample which comprises; forming a complex of the antigen together with (a) multiple immobilized monoclonal antibodies against different epitopes on the antigen; and with (b) a detectably labelled monoclonal soluble antibody which is one of the multiple immobilized antibodies; separating labelled antibody associated with the complex from soluble labelled antibody, and detecting either the labelled antibody associated with the complex, or unassociated labelled antibody; wherein any one of the multiple immobilized monoclonal antibodies shows, by itself, substantially less binding towards the antigen in the immunometric assay, when used with itself or another monoclonal antibody in soluble labelled form, than the multiple immobilized antibodies in combination.
Inventors
- 👤 Jack R. Wands
- 👤 Dominique Bellet
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 10:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; G01N33/575; G01N33/5757; G01N33/577; G01N33/74; G01N33/76
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
114 words
An immunometric assay for a multivalent antigen in a sample which comprises: forming a complex of said antigen together with (a) multiple immobilized monoclonal antibodies against different epitopes on said antigen; and with (b) a detectably labelled monoclonal soluble antibody which is one of said multiple immobilized antibodies; separating labelled antibody associated with said complex from soluble labelled antibody, and detecting either said labelled antibody associated with said complex, or unassociated labelled antibody; wherein any one of said multiple immobilized monoclonal antibodies shows, by itself, substantially less binding towards said antigen in said immunometric assay, when used with itself or another monoclonal antibody in soluble labelled form, than said multiple immobilized antibodies in combination.
Claim 2
Dependent Claim
114 words
An immunometric assay for a multivalent antigen in a sample which comprises: forming a complex of said antigen together with (a) multiple immobilized monoclonal antibodies against different epitopes on said antigen; and with (b) a detectably labelled monoclonal soluble antibody which is one of said multiple immobilized antibodies; separating labelled antibody associated with said complex from soluble labelled antibody, and detecting either said labelled antibody associated with said complex, or unassociated labelled antibody; wherein any one of said multiple immobilized monoclonal antibodies shows, by itself, substantially less binding towards said antigen in said immunometric assay, when used with itself or another monoclonal antibody in soluble labelled form, than said multiple immobilized antibodies in combination.
Claim 3
Dependent Claim
10 words
The assay of claim 1 wherein said antigen is AFP.
Claim 4
Dependent Claim
10 words
The assay of claim 1 wherein said antigen is HCG.
Claim 5
Dependent Claim
105 words
An immunometric assay for AF P which comprises: forming a complex of said alpha-fetoprotein together with (a) at least two immobilized monoclonal antibodies, the first of said antibodies having the identifying characteristics of an antibody obtained from the cell line ATCC No HB 8717; and the second of said antibodies having the identifying characteristics of an antibody obtained from cell line ATCC No HB 8718; and with (b) at least one detectably labelled soluble antibody which is said first antibody; separating labelled antibody associated with said complex from soluble labelled antibody; and detecting either said labelled antibody associated with said complex, or unassociated labelled antibody.
Claim 6
Dependent Claim
105 words
An immunometric assay for AF P which comprises: forming a complex of said alpha-fetoprotein together with (a) at least two immobilized monoclonal antibodies, the first of said antibodies having the identifying characteristics of an antibody obtained from the cell line ATCC No HB 8717; and the second of said antibodies having the identifying characteristics of an antibody obtained from cell line ATCC No HB 8718; and with (b) at least one detectably labelled soluble antibody which is said first antibody; separating labelled antibody associated with said complex from soluble labelled antibody; and detecting either said labelled antibody associated with said complex, or unassociated labelled antibody.
Claim 7
Dependent Claim
107 words
An immunometric assay for human chorionic gonadotropin which comprises: forming a complex of said human chorionic gonadotropin together with (a) at least two immobilized monoclonal antibodies, the first of said antibodies having the identifying characteristics of an antibody obtained from the cell line Pasteur Inst I-297; and the second of said antibodies having the identifying characteristics of an antibody obtained from cell line P asteur Inst. 1-298; and with (b) at least one detectably labelled soluble antibody which is said first antibody; separating labelled antibody associated with said complex from soluble labelled antibody; and detecting either said labelled antibody associated with said complex, or unassociatec labelled antibody.
Claim 8
Dependent Claim
107 words
An immunometric assay for human chorionic gonadotropin which comprises: forming a complex of said human chorionic gonadotropin together with (a) at least two immobilized monoclonal antibodies, the first of said antibodies having the identifying characteristics of an antibody obtained from the cell line Pasteur Inst I-297; and the second of said antibodies having the identifying characteristics of an antibody obtained from cell line P asteur Inst. 1-298; and with (b) at least one detectably labelled soluble antibody which is said first antibody; separating labelled antibody associated with said complex from soluble labelled antibody; and detecting either said labelled antibody associated with said complex, or unassociatec labelled antibody.
Claim 9
Dependent Claim
67 words
A kit useful for the detection of a multivalent antigen in a sample comprising a carrier being compartmentalized to receive in close confinement therein one or more containers wherein (a) a first container contains multiple monoclonal antibodies against different epitopes on said antigen, immobilized on an insoluble solid phase; (b) a second container contains detectably labelled monoclonal soluble antibody which is one of said multiple immobilized antibodies.
Claim 10
Dependent Claim
67 words
A kit useful for the detection of a multivalent antigen in a sample comprising a carrier being compartmentalized to receive in close confinement therein one or more containers wherein (a) a first container contains multiple monoclonal antibodies against different epitopes on said antigen, immobilized on an insoluble solid phase; (b) a second container contains detectably labelled monoclonal soluble antibody which is one of said multiple immobilized antibodies.
Claim 11
Dependent Claim
12 words
The kit of Claim 6 wherein said antigen is AFP or HCG.
Claim 12
Dependent Claim
40 words
The kit of claim 6 wherein said first container contains antibodies derived from cell lines ATCC No HB 8717 and ATCC No HB 8718 and wherein said second container contains antibody derived from cell line AT CC NO HB 8717.
Claim 13
Dependent Claim
37 words
The kit of claim 6 wherein said first container contains antibodies derived from cell lines Pasteur I nst. I-297 and Pasteur Inst. 1-298 and wherein said second container contains antibody derived from cell line Pasteur Inst. 1-297.
Claim 14
Dependent Claim
30 words
An insoluble immunological reagent support useful in an immunometric assay for a multivalent antigen which comprises an insoluble support comprising at least two different immobilized monoclonal antibodies against said antigen.
Claim 15
Dependent Claim
30 words
An insoluble immunological reagent support useful in an immunometric assay for a multivalent antigen which comprises an insoluble support comprising at least two different immobilized monoclonal antibodies against said antigen.
Claim 16
Dependent Claim
14 words
ll. The reagent of claim 10 wherein said antibodies are against AFP or HCG.
Claim 17
Dependent Claim
24 words
The reagent of Claim 10 wherein said antibodies are derived from cell line ATCC NO HB 8717 and cell line ATCC NO HB 8718.
Claim 18
Dependent Claim
22 words
The reagent of Claim 10 wherein said antibodies are derived from cell line Pasteur Inst. I-297 and cell line Pasteur Inst. I-298.
References Cited (Backward Citations)
No citation records indexed.
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