EP0167335A2
Expired / Lapsed
EP
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Method of detecting binding reaction between ligand and anti-ligand
Assignee: Individual
•
Filing: 1985-06-25
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Granted: —
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Expires: 2005-06-25
Abstract
Abstract Stripes 12 of antibody of microscopic dimensons are applied to a substrate surface 10 and antigen in the solution 14 interacts with the antibody in a binding reaction. A laser beam 16 incident on the array of stripes 12 undergoes Bragg scattering and the scattered intensity at the Bragg scattering angle which is detected by detector 30 is representative of the binding reaction which has occurred.
Inventors
- 👤 David F. Nicoli
- 👤 Virgil B. Elings
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 20:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554; G01N21/00; G01N21/17; G01N21/47; G01N21/4788; G01N33/54366; G01N33/54373; G01N2021/4704; G01N2021/4711; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/909
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
135 words
A method for providing an optical detection of a binding reaction between a ligand and an antiligand, including the following steps, forming a predetermined pattern having a spatial array of microscopic dimensions of antiligand material, providing ligand material to interact with the spatial array of antiligand material to produce a binding reaction between the ligand and the antiligand in the periodic pattern, directing optical radiation including energy of at least one wavelength to the pattern to produce scattering of the energy from the pattern following the physical principle of constructive optical interference in accordance with the binding reaction and with a strong scattering intensity at one or more particular scattering angles, and detecting the strong scattering intensity at one or more of the particular scattering angles to produce a signal representative of the binding reaction.
Claim 2
Independent Claim
135 words
A method for providing an optical detection of a binding reaction between a ligand and an antiligand, including the following steps, forming a predetermined pattern having a spatial array of microscopic dimensions of antiligand material, providing ligand material to interact with the spatial array of antiligand material to produce a binding reaction between the ligand and the antiligand in the periodic pattern, directing optical radiation including energy of at least one wavelength to the pattern to produce scattering of the energy from the pattern following the physical principle of constructive optical interference in accordance with the binding reaction and with a strong scattering intensity at one or more particular scattering angles, and detecting the strong scattering intensity at one or more of the particular scattering angles to produce a signal representative of the binding reaction.
Claim 3
Dependent Claim
37 words
The method of claim 1 wherein the predetermined pattern is formed by a plurality of stripes of antiligand material, each stripe having a width of microscopic dimensions and separated from each other by a constant microscopic dimension.
Claim 4
Dependent Claim
24 words
The method of claim 2 wherein the plurality of stripes of antiligand material is formed by antiligand material supported on a flat continuous surface.
Claim 5
Dependent Claim
26 words
The method of claim 1 wherein the spatial pattern of antiligand material is formed by antiligand material supported on a grooved surface forming an interference grating.
Claim 6
Dependent Claim
24 words
The method of claim 1 including ligand attached to carrier particles which competes with the unknown ligand for binding sites on the antiligand material.
Claim 7
Dependent Claim
28 words
The method of claim 1 including antiligand attached to carrier particles to cause a binding reaction with the ligand for increasing the signal representative of the binding reaction.
Claim 8
Independent Claim
32 words
The method of any of the preceding claims wherein the optical detection is on the same side of the pattern as the source of optical radiation to detect backscattered, or reflected, radiation.
Claim 9
Independent Claim
34 words
The method of any of claims 1 to 6 wherein the optical detection is on the opposite side of the pattern as the source of optical radiation to detect forward scattered, or transmitted, radiation.
Claim 10
Dependent Claim
17 words
The method of claim 1 wherein the ligand is attached to carrier particles which are biological entities.
Claim 11
Dependent Claim
15 words
The method of claim 9 wherein the ligand on the carrier particle is naturally occurring.
Claim 12
Dependent Claim
22 words
The method of claim 1 wherein the pattern is formed by deactivating microscopic sections of an initially uniform layer of an antiligand.
Claim 13
Dependent Claim
34 words
The method of claim 11 in which the deactivated sections consist of a plurality of stripes each stripe having a width of microscopic dimensions and separated from each other by a constant microscopic dimension.
Claim 14
Independent Claim
26 words
A method as claimed in any of the preceding claims in which the antiligand material comprises an antibody, the ligand material is an antigen in solution.
Claim 15
Independent Claim
20 words
A method as claimed in any of the preceding claims in which the optical radiation is substantially coherent and monochromatic.
References Cited (Backward Citations)
No citation records indexed.
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