EP0173500A1
Expired / Lapsed
EP
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Methods of concentrating ligands and active membranes used therefor
Assignee: Pall Corp
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Filing: 1985-08-08
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Granted: —
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Expires: 2005-08-08
Abstract
Abstract A chemically active membrane having a large surface area is provided in which a hydrophilic, microporous, skinless, polyamide membrane (12) is chemically bound to a residue of an activating agent which is capable of reacting with a biologically active material. The chemically active membrane, formed by reacting a hydrophilic, microporous, skinless, polyamide membrane (12) with an activating agent may be used to prepare a biologically active membrane (10) having a large surface area which comprises an acceptor molecule such as an monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an anzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, carrier protein, a receptor, heparin, a coagulation factor, or a histone covalently bound to the hydrophilic, microporous, skinless, polyamide membrane (12) by reacting the chemically activate membrane with the acceptor molecule.
Inventors
- 👤 Peter John Degen
- 👤 Jerold Maurice Martin
- 👤 Judy Lynne Schriefer
- 👤 Brenda Winkel Shirley
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 14:02
CPC Classifications
B; B01; B01D; B01D67/00; B01D67/0081; B01D67/0093; A; A61; A61K; A61K39/00; B01D69/00; B01D69/14; B01D69/141; C; C07; C07K; C07K16/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/824; Y10S436/827; Y10S436/828
Claims Analysis
Total Claims: 69
Claim 1
Independent Claim
105 words
A method of preparing a biologically active membrane having a large surface area comprising the steps of: reacting a hydrophilic, microporous, skinless polyamide membrane with an activating agent to form a chemically activated membrane, and reacting said chemically activated membrane with an acceptor molecule to form a biologically active membrane, said acceptor molecule being a member selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone.
Claim 2
Independent Claim
105 words
A method of preparing a biologically active membrane having a large surface area comprising the steps of: reacting a hydrophilic, microporous, skinless polyamide membrane with an activating agent to form a chemically activated membrane, and reacting said chemically activated membrane with an acceptor molecule to form a biologically active membrane, said acceptor molecule being a member selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said polyamide membrane comprises polyhexamethylene adipamide.
Claim 4
Dependent Claim
19 words
The method of claim 2 wherein said membrane is formed from a material modified to include controlled surface properties.
Claim 5
Dependent Claim
17 words
The method of claim 2 wherein said modified polyamide membrane includes a high concentration of carboxyl moieties.
Claim 6
Dependent Claim
43 words
The method of claim 2 wherein said activating agent includes at least one functional group capable of reacting with moieties on the surface of said polyamide membrane to form an activated membrane having a functional group capable of reacting with said acceptor molecule.
Claim 7
Dependent Claim
13 words
The method of claim 2 wherein said acceptor molecule is a monoclonal antibody.
Claim 8
Dependent Claim
11 words
The method of claim 2 wherein said activating agent comprises trichloro-s-triazine.
Claim 9
Dependent Claim
11 words
The method of claim 2 wherein said activating agent comprises N,N'-dicyclohexylcarbodiimide.
Claim 10
Dependent Claim
13 words
The method of claim 2 wherein a substrate is embedded in said membrane.
Claim 11
Independent Claim
60 words
A method for preparing a biologically active membrane having a large surface area comprising the steps of: reacting a hydrophilic, microporous, skinless, polyamide membrane with an activating agent to form a chemically activated membrane wherein said activating agent is either trichloro-s-triazine or N, N'-dicyclohexylcarbodiimide, and reacting said chemically activated membrane with an acceptor molecule to form a biologically active membrane.
Claim 12
Independent Claim
60 words
A method for preparing a biologically active membrane having a large surface area comprising the steps of: reacting a hydrophilic, microporous, skinless, polyamide membrane with an activating agent to form a chemically activated membrane wherein said activating agent is either trichloro-s-triazine or N, N'-dicyclohexylcarbodiimide, and reacting said chemically activated membrane with an acceptor molecule to form a biologically active membrane.
Claim 13
Dependent Claim
23 words
ll. The method of claim 10 wherein said acceptor molecule is capable of reacting with a ligand to form a stable biospecific complex.
Claim 14
Dependent Claim
13 words
The method of claim 10 wherein said acceptor molecule is a proteinaceous substance.
Claim 15
Dependent Claim
61 words
The method of claim 11 wherein said acceptor molecule is a member selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone.
Claim 16
Dependent Claim
71 words
A biologically active membrane having a large surface area comprising an acceptor molecule selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone covalently bound to a hydrophilic, microporous, skinless polyamide membrane.
Claim 17
Dependent Claim
71 words
A biologically active membrane having a large surface area comprising an acceptor molecule selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone covalently bound to a hydrophilic, microporous, skinless polyamide membrane.
Claim 18
Dependent Claim
14 words
The biologically active membrane of claim 14 wherein said polyamide membrane comprises polyhexamethylene adipamide.
Claim 19
Dependent Claim
26 words
The biologically active membrane of claim 15 wherein said polyamide membrane has a methylene CH 2 :amide NHCO ratio of from about 5:1 to about 7:1.
Claim 20
Dependent Claim
18 words
The biologically active membrane of claim 15 wherein said polyamide membrane is modified to include controlled surface properties.
Claim 21
Dependent Claim
19 words
The biologically active membrane of claim 17 wherein said controlled surface properties include a high concentration of carboxyl moieties.
Claim 22
Dependent Claim
26 words
The biologically active membrane of claim 15 wherein a residue of an activating agent covalently links said acceptor molecule to said hydrophilic, microporous, skinless polyamide membrane.
Claim 23
Dependent Claim
19 words
The biologically active membrane of claim 19 wherein said residue of an activating agent comprises a residue of trichloro-s-triazine.
Claim 24
Dependent Claim
27 words
A biologically active membrane having a large surface area comprising an acceptor molecule covalently bound to a hydrophilic, microporous, skinless, polyamide membrane through a residue of trichloro-s-triazine.
Claim 25
Dependent Claim
27 words
A biologically active membrane having a large surface area comprising an acceptor molecule covalently bound to a hydrophilic, microporous, skinless, polyamide membrane through a residue of trichloro-s-triazine.
Claim 26
Dependent Claim
24 words
The biologically active membrane of claim 21 wherein said acceptor molecule is capable of reacting with a ligand to form a stable biospecific complex.
Claim 27
Dependent Claim
16 words
The biologically active membrane of claim 21 wherein said biologically active material is a proteinaceous substance.
Claim 28
Dependent Claim
61 words
The biologically active membrane of claim 21 wherein said acceptor molecule is a member selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, and a coagulation factor.
Claim 29
Dependent Claim
15 words
The biologically active membrane of claim 21 wherein said acceptor molecule is a monoclonal antibody.
Claim 30
Dependent Claim
15 words
The biologically active membrane of claim 15 wherein a substrate is embedded in said membrane.
Claim 31
Dependent Claim
90 words
A filter element comprising the biologically active membrane of claim 26 wherein said membrane is positioned intermediate porous support sheets forming thereby a membrane element and said membrane element is arranged in a tubular corrugated configuration having axially extending pleats, said tubular configured membrane element being placed intermediate a pair of concentric, cylindrical, rigid, perforated tubes, the ends of said tubular configured membrane element and said concentric cylindrical tubes being closed off with end caps forming a fluid-tight seal between said tubular configured membrane element and said concentric perforated tubes.
Claim 32
Independent Claim
90 words
A method for recovering a ligand from a solution including passing a ligand solution through a biologically active membrane comprising an acceptor molecule selected from the group consisting of a monoclonal antibody, a polyclonal anitbody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulins, a carrier protein, a receptor, heparin, a coagulation factor, and a histone covalently bound to a hydrophilic, microporous, skinless polyamide membrane and binding said ligand to said biologically active membrane.
Claim 33
Independent Claim
90 words
A method for recovering a ligand from a solution including passing a ligand solution through a biologically active membrane comprising an acceptor molecule selected from the group consisting of a monoclonal antibody, a polyclonal anitbody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulins, a carrier protein, a receptor, heparin, a coagulation factor, and a histone covalently bound to a hydrophilic, microporous, skinless polyamide membrane and binding said ligand to said biologically active membrane.
Claim 34
Dependent Claim
12 words
The method of claim 28 wherein said polyamide membrane comprises polyhexamethylene adipamide.
Claim 35
Dependent Claim
20 words
The method of claim 29 wherein said polyamide membrane is formed from a material modified to include controlled surface properties.
Claim 36
Dependent Claim
16 words
The method of claim 30 wherein said polyamide membrane includes a high concentration of carboxyl moieties.
Claim 37
Dependent Claim
14 words
The method of claim 29 wherein a substrate is embedded in said polyamide membrane.
Claim 38
Dependent Claim
12 words
The method of claim 29 wherein said ligand is a proteinaceous substance.
Claim 39
Dependent Claim
52 words
The method of claim 29 wherein said ligand is a member of a group consisting of an antigenic substance, a monoclonal antibody, a polyclonal antibody, a lectin, a glycoprotein, a carbohydrate, Protein A, an enzyme, a carrier protein, a receptor, a hormone, a coagulation factor, heparin, a nucleic acid, and a polynucleotide.
Claim 40
Independent Claim
46 words
A method for recovering a ligand from a solution including passing a ligand solution through a biologically active membrane comprising an acceptor molecule covalently bound to a hydrophilic, microporous, skinless, polyamide membrane through a residue of trichloro-s-triazine and binding said ligand to said biologically active membrane.
Claim 41
Independent Claim
46 words
A method for recovering a ligand from a solution including passing a ligand solution through a biologically active membrane comprising an acceptor molecule covalently bound to a hydrophilic, microporous, skinless, polyamide membrane through a residue of trichloro-s-triazine and binding said ligand to said biologically active membrane.
Claim 42
Dependent Claim
61 words
The method of claim 35 wherein said acceptor molecule is a member selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulins, a carrier protein, a receptor, heparin, a coagulation factor, and a histone.
Claim 43
Dependent Claim
13 words
The method of claim 35 wherein said acceptor molecule is a proteinaceous substance.
Claim 44
Dependent Claim
13 words
The method of claim 36 wherein said acceptor molecule is a monoclonal antibody.
Claim 45
Dependent Claim
23 words
The method of claim 29 wherein said acceptor molecule is chemically bound to said polyamide membrane through a residue of an activating agent.
Claim 46
Dependent Claim
11 words
The method of claim 39 wherein said activating agent comprises trichloro-s-triazine.
Claim 47
Dependent Claim
16 words
The method of claim 29 wherein the bound ligand is eluted from said biologically active membrane.
Claim 48
Dependent Claim
13 words
The method of claim 29 wherein a substrate is embedded in said membrane.
Claim 49
Dependent Claim
86 words
The method of claim 42 wherein said membrane is positioned intermediate between porous support sheets, forming thereby a membrane element, and said membrane element is arranged in a tubularical corrugated configuration Lading axially extending pleats, said tubular configured membrane element being placed intermediate a pair of concentric, cylindrical, rigid, perforated tubes, the ends of said concentric, cylindrical tubes and said tubular configured membrane element being closed of with end caps to form a fluid-tight seal between said tubular configured membrane element and said concentric perforated tubes.
Claim 50
Independent Claim
32 words
A method for preparing a chemically activated membrane comprising reacting a hydrophilic, microporous, skinless polyamide membrane with an activating agent derived from trichloro-s-triazine or N, N'-dicyclohexylcarbodiimide to form a chemically activated membrane.
Claim 51
Independent Claim
32 words
A method for preparing a chemically activated membrane comprising reacting a hydrophilic, microporous, skinless polyamide membrane with an activating agent derived from trichloro-s-triazine or N, N'-dicyclohexylcarbodiimide to form a chemically activated membrane.
Claim 52
Dependent Claim
12 words
The method of claim 44 wherein said polyamide membrane comprises polyhexamethylene adipamide.
Claim 53
Dependent Claim
19 words
The method of claim 45 wherein said membrane is formed from a material modified to include controlled surface properties.
Claim 54
Dependent Claim
17 words
The method of claim 46 wherein said modified polyamide membrane includes a high concentration of carboxyl moieties.
Claim 55
Dependent Claim
24 words
The method of claim 45 wherein said polyamide membrane has a methylene CH 2 :amide NHCO ratio of from about 5:1 to about 7:1.
Claim 56
Dependent Claim
16 words
The method of claim 45 wherein said polyamide membrane is modified to include controlled surface properties.
Claim 57
Dependent Claim
17 words
The method of claim 49 wherein said controlled surface properties include a high concentration of carboxyl moieties.
Claim 58
Dependent Claim
13 words
The method of claim 45 wherein a substrate is embedded in said membrane.
Claim 59
Dependent Claim
85 words
The method of claim 51 wherein said membrane is positioned intermediate porous support sheets, forming thereby a membrane element, and said membrane element is arranged in a tubular corrugated configuration having axially extending pleats, said tubular configured membrane element being placed intermediate a pair of concentric, cylindrical, rigid, perforated tubes, the ends of said tubular configured membrane element and said concentric, cylindrical tubes being closed off with end caps to form a fluid-tight seal between said tubular configured membrane element and said concentric perforated tubes.
Claim 60
Dependent Claim
45 words
A chemically active membrane having a large surface area comprising a hydrophilic, microporous, skinless, polyamide membrane chemically bound to a residue of an activating agent, which residue is derived from trichloro-s-triazine or N, N' dicyclohexylcarbodiimide and is capable of reacting with a biologically active material.
Claim 61
Dependent Claim
45 words
A chemically active membrane having a large surface area comprising a hydrophilic, microporous, skinless, polyamide membrane chemically bound to a residue of an activating agent, which residue is derived from trichloro-s-triazine or N, N' dicyclohexylcarbodiimide and is capable of reacting with a biologically active material.
Claim 62
Dependent Claim
14 words
The chemically active membrane of claim 53 wherein said polyamide membrane comprises polyhexamethylene adipamide.
Claim 63
Dependent Claim
26 words
The chemically active membrane of claim 54 wherein ssid polyamide membrane has a methylene CH o :- amide NHCO ratio of about 5:1 to about 7:1.
Claim 64
Dependent Claim
18 words
The chemically active membrane of claim 54 wherein said polyamide membrane is modified to include controlled surface properties.
Claim 65
Dependent Claim
24 words
The chemically active membrane of claim 56 wherein said polyamide membrane includes a high concentration of carboxyl moieties at the surface of said membrane.
Claim 66
Dependent Claim
14 words
The chemically active membrane of claim 54 wherein said residue is derived from trichloro-s-triazine.
Claim 67
Dependent Claim
14 words
The chemically active membrane of claim 54 wherein said residue is derived from N,N'-dicyclohexylcarbodiimide.
Claim 68
Dependent Claim
16 words
60 The chemically active membrane of claim 54 wherein a substrate is embedded in said membrane.
Claim 69
Dependent Claim
91 words
A filter element comprising the chemically active membrane of claim 60 wherein said membrane is positioned intermediate porous support sheets, forming thereby a membrane element, and said membrane element is arranged in a tubular corrugated configuration having axially extending pleats, said tubular configured membrane element being placed intermediate a pair of concentric, cylindrical, rigid, perforated tubes, the open ends of said tubular configured membrane element and said concentric, cylindrical tubes being closed off with end caps forming a fluid-tight seal between said tubular configured membrane element and said concentric, cylindrical tubes.
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