EP0180921A2
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EP
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Process for the preparation and purification of peptides
Assignee: USV Pharmaceutical Corp
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Filing: 1985-10-31
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Granted: —
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Expires: 2005-10-31
Abstract
Abstract Peptides, such as calcitonin, having two cysteine residues connected by a disulfide bond are prepared by a process which includes the step of cyclizing the cysteines at a pH of 8.5 to 9.0, and a concentration of 0.4 to 1.8 mg of peptide per ml of solution. These novel conditions produce rapid cyclization with an unforeseen improvement in yield.
Inventors
- 👤 David Stevenson
- 👤 Mohammad A. Islam
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 14:15
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/02; C07K1/06; C07K1/061; C07K1/067; C07K14/00; C07K14/435; C07K14/575; C07K14/585; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/15; Y10S930/16
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
44 words
A process for forming a disulfide bridge in a peptide containing at least one pair of cysteine moieties which are initially uncyclized, comprising maintaining a solution of the peptide at a pH above 8.5 but not exceeding 9.0, until the cyclization has taken place.
Claim 2
Dependent Claim
44 words
A process for forming a disulfide bridge in a peptide containing at least one pair of cysteine moieties which are initially uncyclized, comprising maintaining a solution of the peptide at a pH above 8.5 but not exceeding 9.0, until the cyclization has taken place.
Claim 3
Dependent Claim
22 words
The process of claim 1 wherein the initially uncyclized peptide is present in a concentration effective to permit the cyclization to occur.
Claim 4
Dependent Claim
19 words
The process of claim 2 wherein said concentration is 0.4 to 1.8 mg of peptide per ml of solution.
Claim 5
Dependent Claim
19 words
The process of claim 2 wherein said concentration is 0.7 to 1.0 mg of peptide per ml of solution.
Claim 6
Dependent Claim
27 words
The process of claim 2 wherein the peptide is a calcitonin having the structure of a naturally occurring calcitonin, or a deletion, substitution, or addition analog thereof.
Claim 7
Dependent Claim
15 words
The process of claim 2 wherein the calcitonin is human calcitonin or an analog thereof.
Claim 8
Dependent Claim
26 words
The process of claim 6 wherein the Cys(l) residue of the initially uncyclized peptide is substituted with an alkyl group containing up to 6 carbon atoms.
Claim 9
Dependent Claim
25 words
A dried product consisting essentially of 1-S-alkyl dihydro-human calcitonin wherein the alkyl group is a straight chain or branched 1 to 6 carbon atoms long.
Claim 10
Dependent Claim
25 words
A dried product consisting essentially of 1-S-alkyl dihydro-human calcitonin wherein the alkyl group is a straight chain or branched 1 to 6 carbon atoms long.
Claim 11
Dependent Claim
11 words
The product of claim 8 wherein the alkyl group is ethyl.
References Cited (Backward Citations)
No citation records indexed.
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