EP0182372A2
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EP
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New factor VIII coagulant polypeptides
Assignee: Scripps Research Institute
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Filing: 1985-11-19
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Granted: —
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Expires: 2005-11-19
Abstract
Abstract Active factor VIII: C coagulant polypeptides identified by partial amino acid sequences are disclosed.
Inventors
- 👤 Theodore S. Zimmerman
- 👤 Carol A. Fulcher
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 09:52
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/36; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/10
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
30 words
An active factor VIII:C coagulant polypeptide having a molecular weight of about 92,000 and having a partial protein amino acid sequence as follows: wherein X and Y are amino acids.
Claim 2
Dependent Claim
30 words
An active factor VIII:C coagulant polypeptide having a molecular weight of about 92,000 and having a partial protein amino acid sequence as follows: wherein X and Y are amino acids.
Claim 3
Dependent Claim
16 words
The polypeptide of Claim 1 wherein amino acids X and Y are ALA and SER, respectively.
Claim 4
Dependent Claim
7 words
An active coagulant polypeptide of the sequence:
Claim 5
Dependent Claim
7 words
An active coagulant polypeptide of the sequence:
Claim 6
Dependent Claim
38 words
An active factor VIII:C coagulant polypeptide having a molecular weight in the range of about 79,000-80,000 and having an active, partial protein amino acid sequence as follows: Wherein Z, A, B, C, D nnd E are amino acids.
Claim 7
Dependent Claim
38 words
An active factor VIII:C coagulant polypeptide having a molecular weight in the range of about 79,000-80,000 and having an active, partial protein amino acid sequence as follows: Wherein Z, A, B, C, D nnd E are amino acids.
Claim 8
Dependent Claim
18 words
The polypeptide of Claim 4 wherein Z, C, D and E are SER, SER, SER and PHE, respectively.
Claim 9
Dependent Claim
17 words
An active coagulant polypeptide of the sequence: wherein A and B are as defined in Claim 4.
Claim 10
Dependent Claim
36 words
A factor VIII:C coagulant polypeptide, thrombin-derived from the polypeptide of Claim 4, having a molecular weight in the range of about 71,000-72,000 and having a partial protein amino acid sequence substantially as shown in Claim 4.
Claim 11
Dependent Claim
19 words
A factor VIII:C coagulant polypeptide, thrombin-derived from the polypeptide of Claim 1, having a molecular weight of about 44,000.
Claim 12
Dependent Claim
19 words
A factor VIII:C coagulant polypeptide, thrombin-derived from the polypeptide of Claim 1, having a molecular weight of about 54,000.
Claim 13
Dependent Claim
54 words
An active factor VIII:C coagulant polypeptide composition consisting essentially a) the polypeptides of Claims 1 and 4; or b) the polypeptides of Claims 1 and 7; or c) the polypeptides of Claims 1, 4 and 7; or d) the polypeptides of Claims 2 and 5; or e) the polypeptides of Claims 3 and 6.
Claim 14
Dependent Claim
67 words
ll. A biological preparation which contains, in combination with an inert carrier, a) the polypeptide of Claim 1; or b) the polypeptide of Claim 4; or c) the polypeptides of Claims 1 and 4; or d) the polypeptides of Claims 1 and 7; or e) the polypeptides of Claims 1, 4 and 7; or f) the polypeptide of Claim 8; or g) the polypeptide of Claim 9.
Claim 15
Dependent Claim
17 words
A biological preparation as claimed in Claim 11 which exhibits specific coagulant activity higher than 1800 units/mg.
Claim 16
Independent Claim
69 words
A method for preparing individual coagulant polypeptides of Claims 1, 4, 7, 8 and 9 from factor VIII:C which comprises a) digesting human factor VIII:C in a digestion mixture with an effective amount of protease under digestion conditions to form individual; b) discontinuing the digestion of step a) while the coagulants are still present in the digestion mixture; and c) isolating the polypeptides so produced according to molecular weight.
References Cited (Backward Citations)
No citation records indexed.
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