EP0186084A2
Expired / Lapsed
EP
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Brain-derived growth factor
Assignee: Merck and Co Inc
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Filing: 1985-12-16
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Granted: —
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Expires: 2005-12-16
Abstract
Abstract Brain-derived acidic fibroblast growth factor, (aFGF), is an active mitogen for vascular endothelial cells in culture and is useful for growth of such cultures for coverage of polymeric vascular grafts, growth of such cultures on tubular supports for production of blood vessels for implantation.
Inventors
- 👤 Kenneth A. Thomas
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 07:57
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/18; A61K38/1825; A61F; A61F2/00; A61F2/02; A61F2/04; A61F2/06; A61K35/00; A61K35/12; A61K35/30; A61L; A61L27/00; A61L27/50; A61L27/507; C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/069; C12N5/0691; C12N2501/00; C12N2501/10; C12N2501/113
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
35 words
A novel method for the stimulation of growth of vascular endothelial cells which comprises treating a sample of the desired endothelial cells in a nutrient medium with aFGF at a concentration of about 1-10 ng/ml.
Claim 2
Dependent Claim
35 words
A novel method for the stimulation of growth of vascular endothelial cells which comprises treating a sample of the desired endothelial cells in a nutrient medium with aFGF at a concentration of about 1-10 ng/ml.
Claim 3
Independent Claim
49 words
A method for the stimulation of the growth of vascular endothelial cells in vitro which comprises treating a sample of vascular endothelial cells in a nutrient medium, comprising 2% or less of bovine serum, glutamine and a broad spectrum antibiotic with aFGF at a concentration of about 1-10 ng/ml.
Claim 4
Independent Claim
49 words
A method for the stimulation of the growth of vascular endothelial cells in vitro which comprises treating a sample of vascular endothelial cells in a nutrient medium, comprising 2% or less of bovine serum, glutamine and a broad spectrum antibiotic with aFGF at a concentration of about 1-10 ng/ml.
Claim 5
Dependent Claim
26 words
The method of Claim 2 wherein cells from a vascular explant are grown on the surface of a synthetic polymeric vessel for implanting in the host.
Claim 6
Dependent Claim
44 words
The method of Claim 2 wherein endothelial cells from a vascular explant are grown on the interior surface of a biocompatible tubular mesh support and smooth muscle cells are grown on the- external surface of the tubular mesh support for implanting in the host.
References Cited (Backward Citations)
No citation records indexed.
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