EP0200541A2
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EP
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Immunochemical method and analytical composition and element for determination of creatine kinase-MB
Assignee: Eastman Kodak Co
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Filing: 1986-04-30
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Granted: —
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Expires: 2006-04-30
Abstract
Abstract An analytical composition and element and an immunochemical method using them, are useful for the determination of creatine kinase-MB in biological fluids. Specific amounts of a P<1>, P<5>-di(adenosine-5 min )polyphosphate, adenosine-5 min -monophosphate (AMP) and adenosine-5 min -diphosphate (ADP) are used to significantly reduce interference by adenylate kinase, especially the liver isoenzyme, in the determination.
Inventors
- 👤 John Bruce Findlay
- 👤 Annie Liu Wu
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 19:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/573; C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/50
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
90 words
An analytical composition for the determination of creatine kinase-MB comprising creatine phosphate and an indicator composition which is capable of providing a detectable change in response to the reaction of creatine phosphate or its reaction product, the composition characterized as comprising adenosine-5'-diphosphate in an amount of from 1 to 3 millimolar, and a combination of adenylate kinase inhibitors consisting essentially of: a) P 1 ,P 5 -di(adenosine-5')polyphosphate present in an amount of at least 0.3 millimolar, and b) adenosine-5'-monophosphate present in a molar ratio to adenosine-5'-diphosphate of at least 10:1.
Claim 2
Dependent Claim
90 words
An analytical composition for the determination of creatine kinase-MB comprising creatine phosphate and an indicator composition which is capable of providing a detectable change in response to the reaction of creatine phosphate or its reaction product, the composition characterized as comprising adenosine-5'-diphosphate in an amount of from 1 to 3 millimolar, and a combination of adenylate kinase inhibitors consisting essentially of: a) P 1 ,P 5 -di(adenosine-5')polyphosphate present in an amount of at least 0.3 millimolar, and b) adenosine-5'-monophosphate present in a molar ratio to adenosine-5'-diphosphate of at least 10:1.
Claim 3
Dependent Claim
102 words
A dry analytical element for the determination of creatine kinase-MB comprising an absorbent carrier material, and containing an analytical composition comprising creatine phosphate, and an indicator composition which is capable of providing a detectable change in response to the reaction of creatine phosphate or its reaction product, the element characterized by comprising adenosine-5'-diphosphate in an amount of from 1 to 3 millimolar, and a combination of adenylate kinase inhibitors consisting essentially of: a) P 1 , P 5_ di(adenosine-5')polyphosphate present in an amount of at least 0.3 millimolar, and b) adenosine-5'-monophosphate present in a molar ratio to adenosine-5'-diphosphate of at least 10:1.
Claim 4
Dependent Claim
102 words
A dry analytical element for the determination of creatine kinase-MB comprising an absorbent carrier material, and containing an analytical composition comprising creatine phosphate, and an indicator composition which is capable of providing a detectable change in response to the reaction of creatine phosphate or its reaction product, the element characterized by comprising adenosine-5'-diphosphate in an amount of from 1 to 3 millimolar, and a combination of adenylate kinase inhibitors consisting essentially of: a) P 1 , P 5_ di(adenosine-5')polyphosphate present in an amount of at least 0.3 millimolar, and b) adenosine-5'-monophosphate present in a molar ratio to adenosine-5'-diphosphate of at least 10:1.
Claim 5
Dependent Claim
21 words
The element as claimed in claim 2 wherein the absorbent carrier material is a porous spreading zone carried on a support.
Claim 6
Dependent Claim
16 words
The element as claimed in claim 3 wherein the spreading zone contains antibodies for creatine kinase-MM.
Claim 7
Independent Claim
128 words
A method for the determination of creatine kinase-MB in an aqueous liquid comprising the steps of: (A) in the presence of at least one antibody for creatine kinase-MM, contacting a sample of a liquid suspected of containing creatine kinase-MB with an analytical composition comprising creatine phosphate, an indicator composition which provides a detectable change in response to the reaction of creatine phosphate or its reaction product, adenosine-5'-diphosphate present in an amount of from 1 to 3 millimolar, and a combination of adenylate kinase inhibitors consisting essentially of: a) P 1 ,P 5- di(adenosine-5')Polyphosphate present in an amount of at least 0.3 millimolar, and b) adenosine-5'-monophosphate present in a molar ratio to adenosine-5'-diphosphate of at least 10:1, and (B) determining the detectable change resulting from the presence of creatine-MB.
Claim 8
Independent Claim
128 words
A method for the determination of creatine kinase-MB in an aqueous liquid comprising the steps of: (A) in the presence of at least one antibody for creatine kinase-MM, contacting a sample of a liquid suspected of containing creatine kinase-MB with an analytical composition comprising creatine phosphate, an indicator composition which provides a detectable change in response to the reaction of creatine phosphate or its reaction product, adenosine-5'-diphosphate present in an amount of from 1 to 3 millimolar, and a combination of adenylate kinase inhibitors consisting essentially of: a) P 1 ,P 5- di(adenosine-5')Polyphosphate present in an amount of at least 0.3 millimolar, and b) adenosine-5'-monophosphate present in a molar ratio to adenosine-5'-diphosphate of at least 10:1, and (B) determining the detectable change resulting from the presence of creatine-MB.
Claim 9
Dependent Claim
15 words
The method as claimed in claim 5 wherein the detectable change is a colorimetric change.
Claim 10
Dependent Claim
33 words
The invention as claimed in any of claims 1 to 6 wherein the P 1 ,P 5 -di(adenosine-5')polyphosphate is P 1 ,P 5 -di(adenosine-5')-pentaphosphate present in an amount of at least 0.5 millimolar.
Claim 11
Dependent Claim
28 words
The invention as claimed in any of claims 1 to 7 wherein the indicator composition provides an optical density change in response to adenosine-5'-triphosphate or its reaction product.
Claim 12
Dependent Claim
30 words
The invention as claimed in any of claims 1 to 7 wherein the indicator composition is a colorimetric indicator composition comprising a substance having peroxidative activity and a leuco dye.
Claim 13
Dependent Claim
25 words
The invention as claimed in any of claims 1 to 9 wherein the adenosine-5'-diphosphate is present in an amount of from 1 to 2 millimolar.
Claim 14
Dependent Claim
23 words
The invention as claimed in any of claims 1 to 10 wherein the molar ratio of adenosine-5'-monophosphate to adenosine-5'-diphosphate is at least 14:1.
References Cited (Backward Citations)
No citation records indexed.
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