EP0215432A2
Expired / Lapsed
EP
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Analysis method and analysis equipment for hop bittering components
Assignee: Shimadzu Corp
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Filing: 1986-09-09
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Granted: —
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Expires: 2006-09-09
Abstract
Abstract An analysis method for hop bittering components which comprises a step of separating and analysing hop bittering components by liquid chromatography, characterized by trapping hop bittering components contained in a sample to be measured in a precolumn consisting of an ester type stationary phase for reversed phase chromatography by passing the sample through the precolumn by means of an acidic aqueous solution containing a polar organic solvent, introducing an eluent for reversed phase chromatography capable of eluting the bittering components into the precolumn, and subjecting the eluate to reversed phase liquid chromatography; and an analysis equipment which is suitable for conducting the above method.
Inventors
- 👤 Miyoko Ono
- 👤 Youichi Kakudo
- 👤 Yasuo Ishida
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 22:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/02; G01N33/14; G01N33/146; G01N30/00; G01N30/02; G01N30/04; G01N30/06; G01N30/08; G01N2030/085
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
92 words
An analysis method for hop bittering components which comprises a step of separating and analysing hop bittering components by liquid chromatography, characterized by trapping hop bittering components contained in a sample to be measured in a precolumn consisting of an ester type stationary phase for reversed phase chromatography by passing the sample through the precolumn by means of an acidic aqueous solution containing a polar organic solvent, introducing an eluent for reversed phase chromatography capable of eluting the bittering components into the precolumn, and subjecting the eluate to reversed phase liquid chromatography.
Claim 2
Dependent Claim
92 words
An analysis method for hop bittering components which comprises a step of separating and analysing hop bittering components by liquid chromatography, characterized by trapping hop bittering components contained in a sample to be measured in a precolumn consisting of an ester type stationary phase for reversed phase chromatography by passing the sample through the precolumn by means of an acidic aqueous solution containing a polar organic solvent, introducing an eluent for reversed phase chromatography capable of eluting the bittering components into the precolumn, and subjecting the eluate to reversed phase liquid chromatography.
Claim 3
Dependent Claim
18 words
An analysis method as claimed in claim 1, wherein the sample to be measured is a hop drink.
Claim 4
Dependent Claim
14 words
An analysis method as claimed in claim 2, wherein the hop drink is beer.
Claim 5
Dependent Claim
20 words
An analysis method as claimed in claim 1, wherein the sample to be measured is hops or a hop product.
Claim 6
Dependent Claim
26 words
An analysis method as claimed in claim-1, wherein the ester type stationary phase for reversed phase chromatography is a statinary phase comprising an acrylate type resin.
Claim 7
Dependent Claim
28 words
An analysis method as claimed in claim 1, wherein the ester type stationary phase for reversed phase chromatography is a stationary phase comprising a tetramethylolmethane triacrylate type resin.
Claim 8
Dependent Claim
42 words
An analysis method as claimed in claim 1, wherein the acidic aqueous solution containing a polar organic solvent is an aqueous solution having a pH of 2 - 4 containing approximately 20 - 40% by volume of a lower alcohol or acetonitrile.
Claim 9
Dependent Claim
23 words
An analysis method as claimed in claim 1, wherein the eluent for reversed phase chromatography is a polar mobile phase containing an alkylammonium.
Claim 10
Dependent Claim
148 words
An analysis equipment for hop bittering components which comprises a flow-line for liquid chromatography having a supply portion for a polar mobile phase containing an alkylammonium, a separation column for reversed phase chromatography and a detector portion, being connected in this order; a flow-line for pretreatment having a supply portion for an acidic aqueous solution containing a polar organic solvent, an introduction portion for a sample containing hop bittering components and a precolumn comprising an ester type stationary phase for reversed phase chromatography, being connected in this order; and a switching flow-line capable of introducing the polar mobile phase into the precolumn on the flow-line for pretreatment and transferring the polar mobile phase to the separation column for reversed phase chromatography through said precolumn, being provided on the flow-line for liquid chromatography between the supply portion for the mobile phase and the separation column for reversed phase chromatography.
Claim 11
Dependent Claim
148 words
An analysis equipment for hop bittering components which comprises a flow-line for liquid chromatography having a supply portion for a polar mobile phase containing an alkylammonium, a separation column for reversed phase chromatography and a detector portion, being connected in this order; a flow-line for pretreatment having a supply portion for an acidic aqueous solution containing a polar organic solvent, an introduction portion for a sample containing hop bittering components and a precolumn comprising an ester type stationary phase for reversed phase chromatography, being connected in this order; and a switching flow-line capable of introducing the polar mobile phase into the precolumn on the flow-line for pretreatment and transferring the polar mobile phase to the separation column for reversed phase chromatography through said precolumn, being provided on the flow-line for liquid chromatography between the supply portion for the mobile phase and the separation column for reversed phase chromatography.
References Cited (Backward Citations)
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