Patent US2242343A successfully refreshed from Google Patents.
EP0215669A2
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Analytical device and method for analysis of biochemicals, microbes and cells
Assignee: Seiko Instruments Inc
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Filing: 1986-09-16
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Granted: —
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Expires: 2006-09-16
Abstract
Abstract An analytical device and a method for the analysis of biochemicals, microbes and cells using a piezoelectic crystal biosensor, utilises the resonant frequency change caused by weight change on the surface of the piezoelectric crystal on which receptor materials is immobilised. Four piezoelectric crystals 161 are mounted in a common cell 164. Each crystal 161 has a pair of electrodes 162 connected by leads 163 to an oscillator circuit 166. The electrodes 162 of three of the crystals 161 are treated to deposit different lectins thereon and those of the fourth left untreated as a reference. A peristaltic pump 169 draws liquid through pipes 165 and cell 164 from a valve 1610 which is solenoid operated to draw from a blood sample supply 1612 or from another valve 1611 drawing from either a phosphate buffer solution reservoir 1613 or a removal agent reservoir 1614. The circuit 166 is connected to a frequency counter 167 whose outputs are fed to a computer 168 which controls the pump 169 and valves 1610 and 1611. The crystals 161 are first resonated to establish their resonant frequencies. A blood sample is then drawn through the cell 164 by pump 169. Type A erythrocytes are attracted to one of the lectins, types A and B to another, and type O to the third. Phosphate buffer is then drawn through by adjustment of valves 1610 and 1611. Resonant frequencies are measured again and compared with those originally obtained. From the changes observed, determination of type is made by the computer. Removal agent is then drawn through to remove remaining erythrocytes and phosphate buffer drawn through to prepare for further samples.
Inventors
- 👤 Isao Karube
- 👤 Hiroshi C/O Seiko Instruments Muramatsu
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 10:34
CPC Classifications
G; G01; G01N; G01N29/00; G01N29/02; G01N29/036; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N2291/00; G01N2291/02; G01N2291/025; G01N2291/0256; Y; Y10; Y10S; Y10S436/00; Y10S436/827; Y10S436/828; Y10S436/829
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
59 words
An analytical device for biochemicals, microbes and cells, comprising a piezoelectric crystal sensor (1,2; 61,62; 141,142, 151,152; 161,162) and means (6,7; 66,67; 166,167) for measuring the resonant frequency of the sensor, the sensor exhibiting a resonant frequency change caused by weight change on the surface of the piezoelectric crystal due to the biochemical, microbe or cell to be analysed.
Claim 2
Dependent Claim
59 words
An analytical device for biochemicals, microbes and cells, comprising a piezoelectric crystal sensor (1,2; 61,62; 141,142, 151,152; 161,162) and means (6,7; 66,67; 166,167) for measuring the resonant frequency of the sensor, the sensor exhibiting a resonant frequency change caused by weight change on the surface of the piezoelectric crystal due to the biochemical, microbe or cell to be analysed.
Claim 3
Dependent Claim
25 words
A device according to claim 1, in which the sensor has receptor material immobilised on or chemically treated onto the surface of the piezoelectric crystal.
Claim 4
Dependent Claim
61 words
An analytical device for biochemicals, microbes and cells, comprising a plurality of piezoelectric crystal sensors (141,142; 151,152; 161,162) and means (166,167) for measuring the resonant frequencies of the sensors at least one of the sensors exhibiting a resonant frequency change caused by weight change on the surface of the piezoelectric crystal due to the biochemical, microbe or cell to be analysed.
Claim 5
Dependent Claim
61 words
An analytical device for biochemicals, microbes and cells, comprising a plurality of piezoelectric crystal sensors (141,142; 151,152; 161,162) and means (166,167) for measuring the resonant frequencies of the sensors at least one of the sensors exhibiting a resonant frequency change caused by weight change on the surface of the piezoelectric crystal due to the biochemical, microbe or cell to be analysed.
Claim 6
Dependent Claim
27 words
A device according to claim 3, in which at least two of the piezoelectric crystals have different receptor materials immobilised on or chemically treated onto their surfaces.
Claim 7
Dependent Claim
30 words
A device according to claim 3 or 4, in which at least one piezoelectric crystal is free from any receptor material immobilised on or chemically treated onto the surface thereof.
Claim 8
Dependent Claim
30 words
A device according to claim 2 or 4, in which biochemical receptor material is selected from sugars, lipids, co-enzymes, amino acids and proteins including enzymes, lectins, antibodies and protein A.
Claim 9
Independent Claim
26 words
A device according to any preceding claim in which the measuring means comprises an oscillator circuit (6; 66; 166) and a frequency counter (7; 67; 167).
Claim 10
Independent Claim
79 words
A method for the analysis of biochemicals, microbes and cells, comprising (a) applying adsorbent biochemicals or organic compunds to a piezoelectric crystal biosensor surface; (b) allowing the biochemicals or organic compund to adsorb biochemicals, microbes or cells to be analysed; (c) measuring the resonant frequency change caused by a weight change on the piezoelectric crystal surface; and (d) detecting the biochemicals, microbes or cells, and determining concentration of the biochemicals, microbes or cells from a calibration curve obtained previously.
Claim 11
Independent Claim
79 words
A method for the analysis of biochemicals, microbes and cells, comprising (a) applying adsorbent biochemicals or organic compunds to a piezoelectric crystal biosensor surface; (b) allowing the biochemicals or organic compund to adsorb biochemicals, microbes or cells to be analysed; (c) measuring the resonant frequency change caused by a weight change on the piezoelectric crystal surface; and (d) detecting the biochemicals, microbes or cells, and determining concentration of the biochemicals, microbes or cells from a calibration curve obtained previously.
Claim 12
Dependent Claim
17 words
A method according to claim 8, in which the piezoelectric crystal is incorporated in a flow cell.
Claim 13
Dependent Claim
18 words
A method according to claim 8 or 9, in which the whole piezoelectric crystal is immersed in liquid.
Claim 14
Dependent Claim
30 words
A method according to claim 8, 9 or 10 in which the measurement is carried out whilst the crystal is subject to a flow of substantially constant temperature distilled water.
Claim 15
Dependent Claim
40 words
A method according to claim 8, 9, 10 or 11 in which fine particles, on which a biochemical or organic compound is immobilised, are applied to attach to the adsorbed biochemicals, microbes or cells on the piezoelectric crystal biosensor surface.
Claim 16
Dependent Claim
14 words
A method according to claim 12, in which the fine particles are of latex.
Claim 17
Independent Claim
22 words
A method according to any of claims 8 to 13, in which the biochemical or organic compound is an antibody or antigen.
Claim 18
Independent Claim
75 words
A method for the analysis of biochemicals, microbes and cells using a piezoelectric crystal biosensor, comprising immobilising at least one material which can adsorb biochemicals, microbes or cells on the surface of the piezoelectric crystal, allowing immobilised material to adsorb the biochemicals, microbes or cells to be analysed, and analysing the constituents of the biochemicals, microbes or cells on the basis of the ability of the immobilised material to adsorb the biochemicals, microbes or cells.
Claim 19
Independent Claim
75 words
A method for the analysis of biochemicals, microbes and cells using a piezoelectric crystal biosensor, comprising immobilising at least one material which can adsorb biochemicals, microbes or cells on the surface of the piezoelectric crystal, allowing immobilised material to adsorb the biochemicals, microbes or cells to be analysed, and analysing the constituents of the biochemicals, microbes or cells on the basis of the ability of the immobilised material to adsorb the biochemicals, microbes or cells.
Claim 20
Independent Claim
68 words
A method for the analysis of biochemicals using a piezoelectric crystal biosensor, comprising immobilising at least one material which can adsorb biochemicals on the surface of the piezoelectric crystal, allowing the immobilised material to adsorb the biochemical to be analysed, flowing an eluent over the surface and analysing the constituents of the biochemical from a change in the resonant frequency of the crystal before and after the elution.
Claim 21
Independent Claim
68 words
A method for the analysis of biochemicals using a piezoelectric crystal biosensor, comprising immobilising at least one material which can adsorb biochemicals on the surface of the piezoelectric crystal, allowing the immobilised material to adsorb the biochemical to be analysed, flowing an eluent over the surface and analysing the constituents of the biochemical from a change in the resonant frequency of the crystal before and after the elution.
Claim 22
Dependent Claim
57 words
A method according to claim 16, in which the immobilised material exhibits different adsorptivities with different biochemicals, in which eluents with differing properties are successively flowed thereover and in which analysis of the constituents of the biochemical is made on the basis of the difference in the adsorptive ability of the immobilised material to adsorb different biochemicals.
Claim 23
Dependent Claim
34 words
A method according to claim 17, in which the eluents are selected fromamong those differing in pH or ionic strength from each other, and containing materials which prevent the adsorption or containing organic solvents.
References Cited (Backward Citations)
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