EP0227173A2
Expired / Lapsed
EP
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New method for the detection of specific binding agents and their corresponding bindable substances
Assignee: Janssen Pharmaceutica NV
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Filing: 1986-12-10
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Granted: —
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Expires: 2006-12-10
Abstract
Abstract A method of qualitatively or quantitatively determining a component of a complex formed between at least one specific binding agent and its corresponding bindable substance. Said method comprises labelling at least one component of said complex with a marker which is easier to determine than the complex itself, whereby the marker used consists of latex particles which can be detected visually either by colour or fluorescence. Preferably the marker consists of coloured or colourable latex particles and the determination is based on the colour characteristics of the latex particles as such or, in the instance that colourable latex particles are used, on the colour characteristics of the coloured particles derived therefrom. The colour, respectively fluorescence signal is, if necessary after development, easily detected and optionally quantified. The invention further comprises coloured or colourable latex particles coated with specific binding proteins and test kits containing the same.
Inventors
- 👤 Nikolaas C.J. De Jaeger
- 👤 Marcel J. Monbaliu
- 👤 Marcus J.M. Noppe
- 👤 Frank J. Konings
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 11:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/58; G01N33/585; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/808; Y10S436/823; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
96 words
A method of qualitatively or quantitatively determining a component of a complex formed between at least one specific binding protein and its corresponding bindable substance which comprises labelling at least one component of said complex with a marker which is easier to determine than the complex iteself, characterized in that the marker used consists of coloured or colourable latex particles and that the determination is based on the colour characteristics of the latex particles as such or, in the instance that colourable latex particles are used, on the colour characteristics of the coloured particles derived therefrom.
Claim 2
Independent Claim
96 words
A method of qualitatively or quantitatively determining a component of a complex formed between at least one specific binding protein and its corresponding bindable substance which comprises labelling at least one component of said complex with a marker which is easier to determine than the complex iteself, characterized in that the marker used consists of coloured or colourable latex particles and that the determination is based on the colour characteristics of the latex particles as such or, in the instance that colourable latex particles are used, on the colour characteristics of the coloured particles derived therefrom.
Claim 3
Independent Claim
29 words
A method according to claim l wherein the marker used consists of coloured latex particles and the determination is based on the colour characteristics of the said coloured particles.
Claim 4
Dependent Claim
73 words
A method according to claim 2 which comprises the steps of: a) immobilizing the said bindable substance on a solid support; b) contacting the support containing the immobilized bindable substance with an aqueous suspension of coloured latex particles coated with binding proteins specific to said bindable substance; and c) observing the coloured signal at the site of interaction, or, optionally, after disengagement, in the resulting suspension or any fraction or phase derived therefrom.
Claim 5
Dependent Claim
40 words
A method according to claim 3 wherein the said bindable substance is immobilized on the said solid support by allowing the said bindable substance to be bound by a specific binding protein which is immobilized on the said solid support.
Claim 6
Dependent Claim
103 words
A method according to claim 2 which comprises the steps of: a) immobilizing the said bindable substance on a solid support; b) contacting the support containing the immobilized bindable substance with a first binding protein specific to said bindable substance to form a complex therewith; c) contacting the support carrying the thus formed complex with an aqueous suspension of coloured latex particles coated with a second binding protein which is specific to the said first binding protein; and d) observing the coloured signal at the site of interaction, or, optionally, after disengagement, in the resulting suspension or any fraction or phase derived therefrom.
Claim 7
Independent Claim
37 words
A method according to claim l wherein the marker consists of colourable latex particles which are chemically converted into coloured latex particles and the determination is based on the colour characteristics of the said coloured latex particles.
Claim 8
Dependent Claim
30 words
A method according to claim 6 wherein the said colourable latex particles comprise recurrent units carrying a moiety capable of oxidatively coupling with an amino compound to form a dye.
Claim 9
Dependent Claim
49 words
A method according to claim 6 wherein the said colourable latex particles comprise recurrent units of a monomer represented by the formula CH₂= -CONH-Q wherein: R is hydrogen, halogen or C 1-4 alkyl; Q is a colour coupler group capable of oxidatively coupling with an aromatic primary amino compound.
Claim 10
Dependent Claim
35 words
A method according to claim 8 wherein the aromatic primary amino compound is a p-phenylene diamine and Q is of the phenol or naphthol type, of the pyrazolone or indazolone or of the acylacetamide type.
Claim 11
Dependent Claim
122 words
l0. A method according to claim 8 wherein the colourable latex comprises a polymer of the formula wherein k is from 30 to 60 and y is from 40 to 70 w/w; or of the formula: wherein n is from 20 to 70, m is from 30 to 60, and p is from 0 to 40; or of the formula: wherein k is from 30 to 60, y is from 20 to 70, and z is from 0 to 40; or of the formula: wherein n is from 20 to 70, m is from 0 to 40, and p is from 30 to 60; or of the formula: wherein n is from 40 to 70 and m is from 30 to 60.
Claim 12
Dependent Claim
91 words
A method according to claim 8, comprising the steps of: a) immobilizing the said bindable substance on a solid support; b) contacting the support containing the immobilized bindable substance with an aqueous suspension of colourable latex particles coated with binding proteins specific to said bindable substance; c) converting the colourable latex particles into coloured particles by oxidatively coupling them with an aromatic primary amine compound; and d) observing the coloured signal at the site of interaction, or, optionally, after disengagement, in the resulting suspension or any fraction or phase derived therefrom.
Claim 13
Independent Claim
40 words
A method according to claim ll, wherein the said bindable substance is immobilized on the said solid support by allowing the said bindable substance to be bound by a specific binding protein which is immobilized on the said solid support.
Claim 14
Dependent Claim
121 words
A method according to claim 8, comprising the steps of: a) immobilizing the said bindable substance on a solid support; b) contacting the support containing the immobilized bindable substance with a first binding protein specific to said bindable substance to form a complex therewith; c) treating the support carrying the thus formed complex with an aqueous suspension of colourable latex particles coated with a secondary binding protein which is specific to the said first binding protein; d) converting the colourable latex particles into coloured particles by oxidatively coupling them with an aromatic primary amine compound; and e) observing the coloured signal at the site of interaction, or, optionally, after disengagement, in the resulting suspension or any fraction or phase derived therefrom.
Claim 15
Dependent Claim
10 words
Coloured or colourable latex particles coated with specific binding proteins.
Claim 16
Dependent Claim
10 words
Coloured or colourable latex particles coated with specific binding proteins.
Claim 17
Dependent Claim
20 words
Coloured or colourable latex particles coated with specific binding proteins for use in the determination of the corresponding bindable substances.
Claim 18
Dependent Claim
20 words
Coloured or colourable latex particles coated with specific binding proteins for use in the determination of the corresponding bindable substances.
Claim 19
Dependent Claim
55 words
Colourable latex particles coated with specific binding proteins, according to claim l4, wherein the particles comprise a polymer containing recurrent units of a monomer represented by the formula CH₂= -CONH-Q wherein: R is hydrogen, halogen or C 1-4 alkyl; Q is a colour coupler group capable of oxidatively coupling with an aromatic primary amino compound.
Claim 20
Dependent Claim
36 words
Colourable latex particles according to claim l6 wherein the aromatic primary amino compound is a p-phenylene diamine and Q is of the phenol or naphthol type, of the pyrazolone or indazolone or of the acylacetamide type.
Claim 21
Dependent Claim
122 words
Colourable latex particles according to claim l4 wherein the colourable latex comprises a polymer of the formula: wherein k is from 30 to 60 and y is from 40 to 70 w/w; or of the formula: wherein n is from 20 to 70, m is from 30 to 60, and p is from 0 to 40; or of the formula: wherein k is from 30 to 60, y is from 20 to 70, and z is from 0 to 40; or of the formula: wherein n is from 20 to 70, m is from 0 to 40, and p is from 30 to 60; or of the formula: wherein n is from 40 to 70 and m is from 30 to 60.
Claim 22
Dependent Claim
33 words
A test kit for the determination of particular bindable substances which comprises, besides other reagents, at least coloured or colourable latex particles coated with binding proteins which are specific to said bindable substances.
Claim 23
Dependent Claim
33 words
A test kit for the determination of particular bindable substances which comprises, besides other reagents, at least coloured or colourable latex particles coated with binding proteins which are specific to said bindable substances.
Claim 24
Dependent Claim
46 words
A test kit for the determination of particular bindable substances which comprises, besides other reagents, at least primary binding proteins which are specific to said bindable substances and coloured or colourable latex particles coated with secondary binding proteins which are specific to said primary binding proteins.
Claim 25
Dependent Claim
46 words
A test kit for the determination of particular bindable substances which comprises, besides other reagents, at least primary binding proteins which are specific to said bindable substances and coloured or colourable latex particles coated with secondary binding proteins which are specific to said primary binding proteins.
References Cited (Backward Citations)
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