EP0229982A2
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EP
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Test device and method of determining concentration of a sample component
Assignee: Bayer Corp
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Filing: 1986-12-11
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Granted: —
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Expires: 2006-12-11
Abstract
Abstract The precision in reflectance or absorption measurements used to determine the presence and concentration of a component in an assay liquid in unexpectedly increased to essentially eliminate the variability of different reactant carriers and variability in reflectance or absorption instruments by including, either within the reactant carrier or within the assay liquid sample, in addition to a primary color forming or color changing indicator, a secondary, inert colorant compound or dye having a light absorption wavelength peak separated from a light absorption wavelength peak of the primary indicator or chromogen by at least 80 nm. To achieve the full advantage of the present invention, the colorant material or marker should be inert to the reactant system in the carrier and should be inert to the assay liquid to provide al light reflectance or light absorption response essentially distinct from the light reflectance response of the primary chromogen to compensate for measurement variability. In this manner, any inaccuracy obtained from a reflectance or absorption measurement of the secondary colorant marker, incorporated into the carrier or into the assay sample in known concentration, can be used for compensation of a reflectance or absorption measurement of the primary color formed as a result of interaction or reaction of the test sample with the reagent composition, and, therefore, the concentration measurement of a predetermined component of the assay liquid.
Inventors
- 👤 Marvin A. Genshaw
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 14:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
115 words
A test article for detecting a relative concentration of a test compound in a test fluid comprising a reagent carrier having homogeneously incorporated therein a reagent composition capable of forming a primary color upon interaction with the test fluid to produce a change in the light reflectance or light absorption of the carrier, and a secondary, inert colorant marker homogeneously incorporated into the carrier or the test fluid in a predetermined concentration, said colorant marker being inert to the test fluid and to the reagent composition and wherein the secondary, inert colorant marker has a light absorption wavelength peak different from a light absorption wavelength peak of the primary color by at least 80 nm.
Claim 2
Dependent Claim
115 words
A test article for detecting a relative concentration of a test compound in a test fluid comprising a reagent carrier having homogeneously incorporated therein a reagent composition capable of forming a primary color upon interaction with the test fluid to produce a change in the light reflectance or light absorption of the carrier, and a secondary, inert colorant marker homogeneously incorporated into the carrier or the test fluid in a predetermined concentration, said colorant marker being inert to the test fluid and to the reagent composition and wherein the secondary, inert colorant marker has a light absorption wavelength peak different from a light absorption wavelength peak of the primary color by at least 80 nm.
Claim 3
Dependent Claim
34 words
The test article of claim 1 wherein the reagent carrier is selected from the group consisting of a foam material, nonwoven fabric, gel film, polymeric film, phase inversion film, and any other liquid-permeable material.
Claim 4
Dependent Claim
52 words
The test article of any of claims 1 and 2 wherein the secondary, inert colorant marker comprises a dye having a high absorption maximum peak separated by at least 120 nm from a light absorption maximum peak of the primary color formed by contact of the test article with a test sample.
Claim 5
Dependent Claim
123 words
A reagent strip for sensing the presence of a chemical compound in an assay liquid, comprising: a carrier material capable of absorbing or adsorbing a liquid reactant composition for uniform distribution of the reactant composition therein; and a reactant composition uniformly distributed throughout the carrier; said reactant composition including a compound capable of interaction with a predetermined component of the assay liquid to form a primary color in the reactant composition to produce a detectable change in the light reflectance or light absorption of the carrier at a predetermined wavelength; said reactant composition also including a secondary, inert colorant marker material having a light absorption wavelength peak different from the light absorption wavelength peak of the primary color by at least 80 nm.
Claim 6
Dependent Claim
123 words
A reagent strip for sensing the presence of a chemical compound in an assay liquid, comprising: a carrier material capable of absorbing or adsorbing a liquid reactant composition for uniform distribution of the reactant composition therein; and a reactant composition uniformly distributed throughout the carrier; said reactant composition including a compound capable of interaction with a predetermined component of the assay liquid to form a primary color in the reactant composition to produce a detectable change in the light reflectance or light absorption of the carrier at a predetermined wavelength; said reactant composition also including a secondary, inert colorant marker material having a light absorption wavelength peak different from the light absorption wavelength peak of the primary color by at least 80 nm.
Claim 7
Dependent Claim
34 words
The reagent strip of claim 4 wherein the reagent carrier is selected from the group consisting of a foam material, nonwoven fabric, gel film, polymeric film, phase inversion film, and any other liquid-permeable material.
Claim 8
Dependent Claim
41 words
The reagent strip of any of claims 4 and 5 wherein the secondary, inert colorant marker comprises a dye having a high absorption maximum peak separated by at least 120 nm from a light absorption maximum peak of the primary color.
Claim 9
Dependent Claim
105 words
In a test device for the quantitative measurement of a compound in a test liquid wherein the test device includes a reagent composition capable of interaction with the compound of the test liquid to produce a primary detectable color in the reagent composition after contact of the reagent composition with the test liquid additionally comprising a secondary colorant material inert to the test liquid and inert to the reagent composition homogeneously incorporated in the reagent composition or in the test liquid in a known concentration and having a light absorption wavelength peak different from that of the primary formed color by at least 80 nm.
Claim 10
Dependent Claim
105 words
In a test device for the quantitative measurement of a compound in a test liquid wherein the test device includes a reagent composition capable of interaction with the compound of the test liquid to produce a primary detectable color in the reagent composition after contact of the reagent composition with the test liquid additionally comprising a secondary colorant material inert to the test liquid and inert to the reagent composition homogeneously incorporated in the reagent composition or in the test liquid in a known concentration and having a light absorption wavelength peak different from that of the primary formed color by at least 80 nm.
Claim 11
Dependent Claim
48 words
The test device of claim 7 wherein the secondary, inert colorant material comprises a dye having a high absorption maximum peak separated by at least 120 nm from a light absorption maximum peak of the primary color formed by contact of the test device with the test liquid.
Claim 12
Independent Claim
235 words
A method of measuring the concentration of a predetermined chemical compound in an assay liquid comprising: distributing a predetermined quantity of a reagent composition into a reagent carrier, wherein the reagent composition includes a compound capable of interaction with a predetermined component of the assay liquid to cause a detectable change in the light reflectance or light absorption of the carrier at a predetermined wavelength by formation of color or a color change in a primary chromogen; said reactant composition also including a secondary, inert colorant marker material having a light absorption wavelength peak different from a light absorption wavelength peak of the primary chromogen by at least 80 nm; drying the carrier; contacting a surface of the dried carrier with the assay liquid to cause color changes detectable at wavelengths characteristic to both the primary chromogen and the secondary colorant in said carrier; detecting the light reflectance or light absorbance of the carrier at a wavelength characteristic of the light reflectance or light absorbance of the primary chromogen, and detecting the light reflectance or light absorbance of the carrier at a wavelength characteristic of the light reflectance or light absorbance of the secondary, inert colorant marker material; and determining the concentration of said predetermined chemical compound based upon the degree of color change in the primary chromogen, as corrected based upon the light reflectance or light absorbance measurement for the secondary colorant marker material.
Claim 13
Independent Claim
235 words
A method of measuring the concentration of a predetermined chemical compound in an assay liquid comprising: distributing a predetermined quantity of a reagent composition into a reagent carrier, wherein the reagent composition includes a compound capable of interaction with a predetermined component of the assay liquid to cause a detectable change in the light reflectance or light absorption of the carrier at a predetermined wavelength by formation of color or a color change in a primary chromogen; said reactant composition also including a secondary, inert colorant marker material having a light absorption wavelength peak different from a light absorption wavelength peak of the primary chromogen by at least 80 nm; drying the carrier; contacting a surface of the dried carrier with the assay liquid to cause color changes detectable at wavelengths characteristic to both the primary chromogen and the secondary colorant in said carrier; detecting the light reflectance or light absorbance of the carrier at a wavelength characteristic of the light reflectance or light absorbance of the primary chromogen, and detecting the light reflectance or light absorbance of the carrier at a wavelength characteristic of the light reflectance or light absorbance of the secondary, inert colorant marker material; and determining the concentration of said predetermined chemical compound based upon the degree of color change in the primary chromogen, as corrected based upon the light reflectance or light absorbance measurement for the secondary colorant marker material.
Claim 14
Dependent Claim
31 words
The method of claim 9 wherein the reflectance or absorption measurements of the carrier are made at the absorption maximums for the primary chromogen and the secondary, inert colorant marker materials.
References Cited (Backward Citations)
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