Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
124 words
A biosensor of the type which comprises an insulative base (12) having an electrode system which includes at least a measuring electrode (14, 14') and a counter electrode (13, 13') and which is covered with a perforated body (18) having an enzyme and an electron acceptor, said perforated body (18) being integrally combined with said electrode system and said base (12), wherein said biosensor electrochemically detects a variation in concentration of a substance occurring during reactions between an oxidoreductase, an electron acceptor and a liquid sample to measure a concentration of a substrate in said sample liquid, characterized in that said electrode system is made primarily of carbon and the surface of at least said measuring electrode is covered with albumin or glucose oxidase.
Claim 2
Dependent Claim
124 words
A biosensor of the type which comprises an insulative base (12) having an electrode system which includes at least a measuring electrode (14, 14') and a counter electrode (13, 13') and which is covered with a perforated body (18) having an enzyme and an electron acceptor, said perforated body (18) being integrally combined with said electrode system and said base (12), wherein said biosensor electrochemically detects a variation in concentration of a substance occurring during reactions between an oxidoreductase, an electron acceptor and a liquid sample to measure a concentration of a substrate in said sample liquid, characterized in that said electrode system is made primarily of carbon and the surface of at least said measuring electrode is covered with albumin or glucose oxidase.
Claim 3
Dependent Claim
32 words
A biosensor according to claim 1, wherein said electrode system is covered by a liquid retaining layer (23), said perforated body (18) and a sample addition layer (20) superposed in this order.
Claim 4
Dependent Claim
22 words
A biosensor according to Claim 2, wherein a filtration layer (21) is provided on the liquid-retaining layer (23) covering said electrode system.
Claim 5
Dependent Claim
110 words
A biosensor according to Claim 2, wherein the electrode system mounted on said insulative base is covered with the liquid-retaining layer (23) whose height is regulated with two resin plates (22); a filtration layer (21), said perforated body (18) and said sample addition layer (20) which are held by a resin holding frame (17) are placed on the liquid-retaining layer (23), and said holding frame (17) is fixed to said base; and a resin cover (19) is provided on the upper surface of said holding frame (17) and has an opening whose diameter is smaller than the outer diameters of said perforated body (18) and said sample addition layer (20).
Claim 6
Dependent Claim
106 words
A method for producing a biosensor according to Claim 1, comprising: providing an insulative base, printing or applying a carbon paste on said base to form an electrode system including at least a measuring electrode and a counter electrode, polishing the surface of the respective electrodes, subjecting the polished electrodes to a thermal treatment at a temperature of 60-170 ° C for 1-8 hours, covering the electrode system partially with albumin or glucose oxidase by adsorption, covering the electrode system with a perforated body having an enzyme and an electron acceptor therein, and integrally combining said perforated body with said electrode system and said insulative base.
Claim 7
Dependent Claim
28 words
A method for producing a biosensor according to Claim 5, wherein the thermal treatment is effected at a temperature of 70 to 150 ° C for 4 hours.