EP0230786B1
Expired / Lapsed
EP
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Assay for cholesterol and derivatives thereof
Assignee: Medisense Inc
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Filing: 1986-12-24
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Granted: 1991-09-25
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Expires: 2006-12-24
Abstract
Abstract A cholesterol sensor is a dry strip sensor which comprises an elongate substrate (l) with a screen printed reference electrode (6) and a screen printed working electrode (5), the working electrode comprising a conducting surface carrying optional cholesterol esterase, a cholesterol oxidase, a peroxidase, and a mediator which transfers charge between the conducting surface and the peroxidase when the peroxide is catalytically active.
Inventors
- 👤 Hugh Allen Oliver Hill
- 👤 Jane Elizabeth Frew
- 👤 Michael Richard Ball
- 👤 Monika Joanna Green
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 20:15
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; C12Q1/001; C12Q1/004
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
93 words
A sensor for use in the assay of free or total cholesterol in blood, the sensor being a dry strip sensor for connection to read-out means and comprising an elongate electrically-insulating substrate with a screen printed reference electrode and a screen printed working electrode, the working electrode comprising a conducting surface carrying a surfactant, a cholesterol oxidase, a peroxidase, and a mediator which transfers electrons between the conducting surface and the peroxidase in the presence of peroxide as substrate, and further carrying cholesterol esterase in the case of an assay for total cholesterol.
Claim 2
Dependent Claim
70 words
The sensor of claim 1, which comprises the elongate, electrically-insulating substrate having a pair of longitudinal, substantially parallel, electrically-conducting tracks thereupon, each track being provided at the same end thereof with means for electrical connection with the read-out means and provided at the other end thereof with an electrode, one of the electrodes being the reference electrode and the other being the working electrode responsive to the presence of cholesterol.
Claim 3
Dependent Claim
21 words
An assay for free or total cholesterol, wherein the cholesterol is amperometrically quantitated using a sensor as defined in claim 1.
References Cited (Backward Citations)
No citation records indexed.
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