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EP0237150A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Improved radionuclide antibody coupling

Assignee: Poniard Pharmaceuticals Inc
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Filing: 1987-01-19
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Granted: —
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Expires: 2007-01-19
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Abstract

Abstract Metal radionuclide labeled proteinaceous sub­stances which contain, in their native states, disulfide (cysteine) linkages, which find use in diagnosis and treatment of a variety of pathologic conditions. The radionuclide labeled proteinaceous substances of the present invention are formed by the treatment of the proteinaceous substance of interest with a disulfide reducing agent, followed by reaction of the reduced proteinaceous substance ("H-treated product") with a suitable radionuclide species containing, for example, 99m Tc, ¹⁸⁶Re and ¹⁸⁸Re, or ⁶⁷Cu. It has been found that the H-treated products, e.g. , antibodies, react specifically with radionuclide species to form stable radionuclide labeled antibodies or other radiolabeled products. The H-treated products may be stabilized or modified in a variety of ways prior to and subsequent to complexation with the metal radionuclide.

Inventors

  • 👤 John M. Reno
  • 👤 Becky J. Bottino

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-04 08:51
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/081; A61K51/10; A61K2121/00; A61K2123/00; Y; Y10; Y10S; Y10S424/00; Y10S424/06; Y10S435/00; Y10S435/81

Claims Analysis

Total Claims: 10
Claim 1
Independent Claim 66 words
A method of preparing a metal radionuclide-­labelled protein from a protein precursor having at least one disulfide linkage, the method comprising: reacting said protein precursor with a disulfide reducing agent to form a dimercaptoprotein; freeing said dimercaptoprotein of spent and unreacted reducing agent to provide purified dimercapto­protein; and combining said purified dimercaptoprotein, substantially free of protein precursor, with a metal radionuclide to form said radionuclide-labelled protein.
Claim 2
Independent Claim 66 words
A method of preparing a metal radionuclide-­labelled protein from a protein precursor having at least one disulfide linkage, the method comprising: reacting said protein precursor with a disulfide reducing agent to form a dimercaptoprotein; freeing said dimercaptoprotein of spent and unreacted reducing agent to provide purified dimercapto­protein; and combining said purified dimercaptoprotein, substantially free of protein precursor, with a metal radionuclide to form said radionuclide-labelled protein.
Claim 3
Independent Claim 44 words
A method of preparing a storage stable metal radionuclide complexing protein comprising the steps of: reacting a disulfide containing protein precurosor with a disulfide reducing agent to form a dimercaptoprotein; and freeing said dimercaptoprotein of spent and unreacted reducing agent to provide purified dimercapto­protein.
Claim 4
Independent Claim 44 words
A method of preparing a storage stable metal radionuclide complexing protein comprising the steps of: reacting a disulfide containing protein precurosor with a disulfide reducing agent to form a dimercaptoprotein; and freeing said dimercaptoprotein of spent and unreacted reducing agent to provide purified dimercapto­protein.
Claim 5
Independent Claim 23 words
The method of any one of preceding claims wherein said protein precursor is a monoclonal or polyclonal antibody or specific bonding fragment thereof.
Claim 6
Independent Claim 37 words
The method of any one of preceding claims comprising the step of reacting the purified dimer­captoprotein with a weakly binding cation or a derivatizing agent for mercapto groups to produce a derivatized product regenerable to the dimercaptoprotein.
Claim 7
Dependent Claim 10 words
The method of claim 4 further comprising regenerating said dimercaptoprotein.
Claim 8
Dependent Claim 19 words
The said method of any one of preceding claims wherein said metal radionuclide includes 99m Tc, ¹⁸⁶Re, or ⁶⁷Cu.
Claim 9
Dependent Claim 11 words
A product of the method of any of the preceding claims.
Claim 10
Dependent Claim 15 words
A kit comprising a product of the method of any of claims l to 6.

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