EP0239990A2
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EP
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Analytical element
Assignee: Konica Minolta Inc
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Filing: 1987-03-31
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Granted: —
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Expires: 2007-03-31
Abstract
Abstract An analytical element for analyzing a specific component in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent and at least one reagent capable of converting said oxidized type coenzyme to a reduced type coenzyme through a specific component in a liquid sample, wherein said oxidized type coenzyme is contained in said porous spreading layer, and a lactate dehydrogenase inhibitor is contained in at least one of said second reagent layer and said porous spreading layer. According to this invention, error originating from LDH and lactic acid present in a biological liquid sample can be eliminated and the accuracy of analysis can be improved.
Inventors
- 👤 Morio Kobayashi
- 👤 Seiji Hidaka
- 👤 Takashi Ishihara
- 👤 Isao Haga
- 👤 Hiroshi Iwadate
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 03:09
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/40; C12Q1/26; C12Q1/32; C12Q1/48; C12Q1/50; C12Q1/52; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; C12Q2326/00; C12Q2326/90; C12Q2326/92; G01N2333/00; G01N2333/90; G01N2333/902; G01N2333/904; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
116 words
An analytical element for analyzing a specific component in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent and at least one reagent capable of converting said oxidized type coenzyme to a reduced type coenzyme through a specific component in a liquid sample, wherein said oxidized type coenzyme is contained in said porous spreading layer, and a lactate dehydrogenase inhibitor is contained in at lest one of said second reagent layer and said porous spreading layer.
Claim 2
Dependent Claim
116 words
An analytical element for analyzing a specific component in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent and at least one reagent capable of converting said oxidized type coenzyme to a reduced type coenzyme through a specific component in a liquid sample, wherein said oxidized type coenzyme is contained in said porous spreading layer, and a lactate dehydrogenase inhibitor is contained in at lest one of said second reagent layer and said porous spreading layer.
Claim 3
Dependent Claim
41 words
The analytical element according to Claim 1, wherein said dye-forming precursor material is contained in the first reagent layer, said buffering agent is contained in the second reagent layer and said lactate dehydrogenase inhibitor is contained in said porous spreading layer.
Claim 4
Dependent Claim
19 words
The analytical element according to Claim 1, wherein said oxidized type coenzyme is an oxidized type nicotinamide adenine dinucleotide.
Claim 5
Dependent Claim
70 words
The analytical element according to Claim 1, wherein said lactate dehydrogenase inhibitor is selected from the group consisting of oxalic acid and a salt thereof, pyruvic acid and a salt thereof, a malonic acid and a salt thereof, oxamic acid and a salt thereof, tartronic acid and a salt thereof, ethylenediaminetetraacetic acid and a salt thereof, iodoacetamide, 2,4-dinitrofluorobenzene, mercuric (II) p-chlorobenzoate, an iodide, a silver salt, and a mercuric salt.
Claim 6
Dependent Claim
26 words
The analytical element according to Claim 1, wherein said specific component is glutamate-oxaloacetate transaminase (GOT), glutamate-pyruvate transaminase (GPT), amilase (AMY), creatine phosphokinase (CPK) or triglyceride (TG).
Claim 7
Dependent Claim
23 words
The analytical element according to Claim 1, wherein said electron transport agent is N-methylphenadine methosulfate, 1-methoxy-N-methylphenadine methosulfate, Meldra's Blue, Methylene Blue or diaphorase.
Claim 8
Dependent Claim
16 words
The analytical element according to Claim 1, wherein said dye-forming precursor material is a tetrazolium salt.
Claim 9
Dependent Claim
29 words
The analytical element according to Claim 7, wherein said tetrazolium salt is 3,3ʹ-(3,3ʹ-dimethoxy-4,4ʹ-biphenylene)-bis[2-(p-nitrophenyl)-5-phenyltetrazolium chloride], 3,3ʹ-(3,3ʹ-dimethoxy-4,4ʹ-biphenylene)-bis[2,5-diphenyltetrazolium chloride], 3-(4ʹ,5ʹ-dimethyl-2-thiazolyl)-2,4-diphenyltetrazolium bromide], 3-(p-iodophenyl)-2-(p-nitrophenyl)-5-phenyltetrazolium chloride, 2,2ʹ-5,5ʹ-(p-nitrophenyl)-3,3ʹ-(3,3ʹ-dimethoxy-4,4ʹ-biphenylene)ditetrazolium chloride, 2,3,5-triphenyltetrazolium chloride, 3,3ʹ-(3,3ʹ-dimethoxy-4,4ʹ-biphenylene)-bis-[2,5-bis(p-nitrophenyl)tetrazolium chloride] or 3,3ʹ-(4,4ʹ-biphenylene)-bis[2,5-diphenyltetrazolium chloride].
Claim 10
Dependent Claim
41 words
The analytical element according to Claim 1, wherein a binder which constitutes said first reagent layer is a water soluble polymer and a binder which constitutes said second reagent layer is a hydrophilic polymer simultaneously having solubility in an organic solvent.
Claim 11
Dependent Claim
56 words
The analytical element according to Cliam 1, wherein said electron transport agent is contained in said analytical element in an amount of 1mg/m² to 1 g/m², except for the case where diaphorase is used, and when diaphorase is used as the electron transport agent, it is contained in an amount of 100 U/m² to 100,000 U/m².
Claim 12
Dependent Claim
56 words
The analytical element according to Cliam 1, wherein said electron transport agent is contained in said analytical element in an amount of 1mg/m² to 1 g/m², except for the case where diaphorase is used, and when diaphorase is used as the electron transport agent, it is contained in an amount of 100 U/m² to 100,000 U/m².
Claim 13
Dependent Claim
28 words
The analytical element according to Cliam 1, wherein said color forming precursor material is contained in said analytical element in an amount of 10 mg/m² to 10 g/m².
Claim 14
Dependent Claim
28 words
The analytical element according to Cliam 1, wherein said color forming precursor material is contained in said analytical element in an amount of 10 mg/m² to 10 g/m².
Claim 15
Dependent Claim
27 words
The analytical element according to Cliam 1, wherein said oxidized type coenzyme is contained in said analytical element in an amount of 10 mg/m² to 50 g/m².
Claim 16
Dependent Claim
27 words
The analytical element according to Cliam 1, wherein said oxidized type coenzyme is contained in said analytical element in an amount of 10 mg/m² to 50 g/m².
Claim 17
Dependent Claim
26 words
The analytical element according to Cliam 1, wherein said LDH inhibitor is contained in said analytical element in an amount of 5 mg/m² to 50 g/m².
Claim 18
Dependent Claim
26 words
The analytical element according to Cliam 1, wherein said LDH inhibitor is contained in said analytical element in an amount of 5 mg/m² to 50 g/m².
Claim 19
Dependent Claim
20 words
The analytical element according to Cliam 1, wherein the film thickness of said spreading layer is 100 to 500 µm.
Claim 20
Dependent Claim
20 words
The analytical element according to Cliam 1, wherein the film thickness of said spreading layer is 100 to 500 µm.
Claim 21
Dependent Claim
19 words
The analytical element according to Cliam 1, wherein the porosity of said spreading layer is 20 to 85 %.
Claim 22
Dependent Claim
19 words
The analytical element according to Cliam 1, wherein the porosity of said spreading layer is 20 to 85 %.
Claim 23
Dependent Claim
104 words
An analytical element for analyzing glutamateoxaloacetate transaminase (GOT) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of aspartic acid, α-ketoglutaric acid and glutamate dehydrogenase (GℓDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 24
Dependent Claim
104 words
An analytical element for analyzing glutamateoxaloacetate transaminase (GOT) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of aspartic acid, α-ketoglutaric acid and glutamate dehydrogenase (GℓDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 25
Dependent Claim
104 words
An analytical element for analyzing glutamateoxaloacetate transaminase (GOT) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of aspartic acid, α-ketoglutaric acid and glutamate dehydrogenase (GℓDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 26
Dependent Claim
104 words
An analytical element for analyzing glutamateoxaloacetate transaminase (GOT) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of aspartic acid, α-ketoglutaric acid and glutamate dehydrogenase (GℓDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 27
Dependent Claim
103 words
An analytical element for analyzing glutamatepyruvate transaminase (GPT) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of alanine, α-ketoglutaric acid and glutamate dehydrogenase (GℓDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 28
Dependent Claim
103 words
An analytical element for analyzing glutamatepyruvate transaminase (GPT) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of alanine, α-ketoglutaric acid and glutamate dehydrogenase (GℓDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 29
Dependent Claim
112 words
An analytical element for analyzing amylase (AMY) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of maltopentose, orthophosphoric acid, β-phosphoglucomutase (β-PGM), glucose oxidase (GOD) and maltose phosphorylase (MP), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid, a salt thereof, pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 30
Dependent Claim
112 words
An analytical element for analyzing amylase (AMY) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of maltopentose, orthophosphoric acid, β-phosphoglucomutase (β-PGM), glucose oxidase (GOD) and maltose phosphorylase (MP), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid, a salt thereof, pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 31
Dependent Claim
111 words
An analytical element for analyzing creatine phosphokinase (CPK) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of creatine, adenosine triphosphate (ATP), hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid, a salt thereof, pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 32
Dependent Claim
111 words
An analytical element for analyzing creatine phosphokinase (CPK) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, a combination of creatine, adenosine triphosphate (ATP), hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid, a salt thereof, pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 33
Dependent Claim
127 words
An analytical element for analyzing triglyceride (TG) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, at least one of a combination of lipoprotein lipase (LPL), glycerokinase (GK), adenosine triphosphate (ATP) and glycerosphosphate dehydrogenase (GPDH), and a combination of lipoprotein lipase (LPL) and glycerol dehydrogenase (GDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid, a salt thereof, pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
Claim 34
Dependent Claim
127 words
An analytical element for analyzing triglyceride (TG) in a liquid sample, comprising a light-transmissive and liquid-impermeable support having thereon successively a first reagent layer and a second reagent layer, and a porous spreading layer provided above said second reagent layer, and containing an electron transport agent, a dye-forming precursor material, an oxidized type coenzyme, a buffering agent, and, as said reagent, at least one of a combination of lipoprotein lipase (LPL), glycerokinase (GK), adenosine triphosphate (ATP) and glycerosphosphate dehydrogenase (GPDH), and a combination of lipoprotein lipase (LPL) and glycerol dehydrogenase (GDH), wherein said oxidized type coenzyme is contained in said porous spreading layer, and oxalic acid, a salt thereof, pyruvic acid or a salt thereof is contained in said porous spreading layer as a lactate dehydrogenase inhibitor.
References Cited (Backward Citations)
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