EP0241904A2
Expired / Lapsed
EP
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Gel scanner
Assignee: Teledyne Isco Inc
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Filing: 1987-04-14
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Granted: —
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Expires: 2007-04-14
Abstract
Abstract To scan gels optically, a gel scanner includes a gel holder and an optical system mounted for motion with respect to each other. The optical system includes a tungsten halogen lamp, first aperture, second aperture, beam splitter and first and second light paths; the first aperture having an aperture with a dimension in the direction of motion of the gel with respect to the optical system of less than 0.5 millimeter and a dimension in a direction transverse to the direction of motion of less than l0 millimeters. The lamp and first aperture are mounted on one side of said gel holder and the second aperture is mounted on the other side of said gel holder. The beam splitter transmits light in a first direction along the first light path and in a second direction along a second light path with the: (l) first light path including photodetector and an interference filter for passing light within the range of 400 nanometers wavelength to 700 nanometers wavelength to it; and (2) the second light path having a second photodetector.
Inventors
- 👤 Robert W. Allington
- 👤 Lawrence L. Sedlak
- 👤 Wylee D. Brunken
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 14:00
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01N21/00; G01N21/17; G01N21/59; G01N21/5907; G01N21/5911
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
221 words
A gel scanner (40) comprising: a gel holder (90); an optical system (l0); a scanner (52, 92) for moving the gel holder (90) and optical system (l0) with respect to each other; said optical system (l0) including a source of light (26), a first aperture, a second aperture (28, l8), a beam splitter (20) and first and second light paths; characterized in that the source of light (26) and first aperture (28) are mounted on one side of said gel holder (92); said second aperture (l8) being mounted on the other side of said gel holder (92); said first and second apertures (28, l8) having dimensions in the direction of motion of less than 0.5 millimeter and in a direction transverse to the direction of motion of less than l0 millimeters; the beam splitter (20) being positioned to transmit light in a first direction along said first light path and light in a second direction along a second light path; said first light path including a first photodetector (32) and filter (30) for selecting light within the range of 400 nanometers wavelength to 700 nanometers wavelength for application to said first photodetector; said second light path having a second photodetector (36); and a monitor for comparing signals from said first and second photodetector to obtain a signal related to said bands.
Claim 2
Dependent Claim
221 words
A gel scanner (40) comprising: a gel holder (90); an optical system (l0); a scanner (52, 92) for moving the gel holder (90) and optical system (l0) with respect to each other; said optical system (l0) including a source of light (26), a first aperture, a second aperture (28, l8), a beam splitter (20) and first and second light paths; characterized in that the source of light (26) and first aperture (28) are mounted on one side of said gel holder (92); said second aperture (l8) being mounted on the other side of said gel holder (92); said first and second apertures (28, l8) having dimensions in the direction of motion of less than 0.5 millimeter and in a direction transverse to the direction of motion of less than l0 millimeters; the beam splitter (20) being positioned to transmit light in a first direction along said first light path and light in a second direction along a second light path; said first light path including a first photodetector (32) and filter (30) for selecting light within the range of 400 nanometers wavelength to 700 nanometers wavelength for application to said first photodetector; said second light path having a second photodetector (36); and a monitor for comparing signals from said first and second photodetector to obtain a signal related to said bands.
Claim 3
Dependent Claim
22 words
Apparatus according to claim 2 characterized in that the filter (30) passes light within a wavelength range of 400 to 700 nanometers.
Claim 4
Dependent Claim
18 words
Apparatus according to either claim l or 2 characterized in that the filter (30) is an interference filter.
Claim 5
Dependent Claim
32 words
Apparatus according to any of claims l-3 characterized by a second filter (34) in the second light path for passing infrared light in the bandwidth range of 640 nanometers to 5 microns.
Claim 6
Dependent Claim
29 words
Apparatus according to claim 4 characterized in that said interference filter (30) has a pass band centered at a wavelength longer than 560 nanometers and shorter than 630 nanometers.
Claim 7
Independent Claim
158 words
A method of scanning dyed gel having bands resolved within it by moving the gel and an optical system with respect to each other; characterized by transmitting light from a source (26) of light through an aperture in an aperture plate (l8) having a dimension in the direction of motion of less than 0.5 millimeter and a dimension perpendicular to the direction of motion of less than l0 millimeters; receiving light on the other side of the gel through an aperture plate (e.g. 28) and transmitting only frequencies of light within the visible range and within the absorption spectrum of the bands to a first photocell; and transmitting other frequencies of the light passing through the gel to a reference photocell, which other frequencies of light includes light only of frequencies not transmitted to the sensing photocell and having a bandwidth of frequencies at least l0 times wider than the bandwidth of frequencies reaching the said first photocell.
Claim 8
Independent Claim
158 words
A method of scanning dyed gel having bands resolved within it by moving the gel and an optical system with respect to each other; characterized by transmitting light from a source (26) of light through an aperture in an aperture plate (l8) having a dimension in the direction of motion of less than 0.5 millimeter and a dimension perpendicular to the direction of motion of less than l0 millimeters; receiving light on the other side of the gel through an aperture plate (e.g. 28) and transmitting only frequencies of light within the visible range and within the absorption spectrum of the bands to a first photocell; and transmitting other frequencies of the light passing through the gel to a reference photocell, which other frequencies of light includes light only of frequencies not transmitted to the sensing photocell and having a bandwidth of frequencies at least l0 times wider than the bandwidth of frequencies reaching the said first photocell.
Claim 9
Dependent Claim
43 words
A method according to claim 6 characterized in that the step of transmitting only frequencies of the light within the visible range includes the step of transmitting only light having wavelengths within the range of 400 to 700 nanometers to the sensing photocell.
References Cited (Backward Citations)
No citation records indexed.
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