EP0244999A2
Expired / Lapsed
EP
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Use of charged particles in the membrane filtration of liquid cell culture media
Assignee: Rohm and Haas Co
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Filing: 1987-04-27
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Granted: —
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Expires: 2007-04-27
Abstract
Abstract There is provided a method of separating components of a liquid cell culture medium, involving mixing together the cell culture medium, first charged, particulate material and optionally second charged particulate material bearing a charge opposite that of the first charged, particulate material to form a suspension of matter in the medium and then recovering the suspended matter using membrane filtration. The method enables increased flow rates through the membrane filter and increased product recovery to be obtained.
Inventors
- 👤 Joaquim Manuel Sampio Cabral
- 👤 Charles Leland Cooney
- 👤 Elizabeth Marie Robinson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 13:05
CPC Classifications
C; C12; C12M; C12M47/00; C12M47/02; C12M33/00; C12M33/14; C12N; C12N1/00; C12N1/02
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
74 words
A method of separating components of a liquid cell culture medium by membrane filtration, which comprises: (a) mixing together the medium and an effective amount of first charged, particulate material to form a suspension of matter in the medium, the charged, particulate material having an average diameter of from about 0.01 to about 2.5 micrometers and bearing 0.1 to 1.5 functional groups per monomer unit; and (b) recovering the suspended matter using membrane filtration.
Claim 2
Independent Claim
74 words
A method of separating components of a liquid cell culture medium by membrane filtration, which comprises: (a) mixing together the medium and an effective amount of first charged, particulate material to form a suspension of matter in the medium, the charged, particulate material having an average diameter of from about 0.01 to about 2.5 micrometers and bearing 0.1 to 1.5 functional groups per monomer unit; and (b) recovering the suspended matter using membrane filtration.
Claim 3
Dependent Claim
25 words
A method as claimed claim 1, wherein the liquid cell culture medium comprises fermentation broth, for example, broth of a bacterium or of a yeast.
Claim 4
Dependent Claim
24 words
A method as claimed in claim 1 or claim 2, wherein the first charged, particulate material comprises ion exchange resin, e.g. anion exchange resin.
Claim 5
Independent Claim
68 words
A method as claimed in any preceding claim, wherein, prior to step (b), the suspension formed in stap (a) and an effective amount of second charged particulate material, e.g. ion exchange resin, bearing a charge opposite that of the first charged, particulate material, bearing 0.1 to 1.5 functional groups per monomer unit, and having an average diameter of from about 0.01 to about 2.5 micrometers, are mixed together.
Claim 6
Dependent Claim
27 words
A method as claimed in claim 4, wherein the first charged, particulate material comprises anion exchange resin and the second charged, particulate material comprises cation exchange resin.
Claim 7
Dependent Claim
43 words
A method as claimed in claim 5, wherein the first and second charged, particulate materials are each present in an amount of about 0.01 to 0.5, for example, about 0.05 to 0.5%, by volume, based on the volume of the cell culture medium.
Claim 8
Dependent Claim
27 words
A method as claimed in claim 4, wherein the first charged particulate material comprises strongly basic resin and the second charged particulate material comprises strongly acidic resin.
Claim 9
Independent Claim
52 words
A method as claimed in any of claims 4 to 7, wherein solid components of the medium, subsequent to mixing of the liquid medium with the first and second charged, particulate material, are separated from the medium and resuspended in a second, aqueous, liquid medium, which is subequently subjected to membrane filtration.
Claim 10
Independent Claim
46 words
A method as claimed in any of claims 4 to 7, wherein solid components of the medium, subsequent to mixing of the liquid medium with the first and second charged, particulate materials, are separated from the medium and the medium is subsequently subjected to membrane filtration.
Claim 11
Independent Claim
24 words
A method as claimed in any preceding claim, wherein the first particulate material has an average diameter of from about 0.01 to 1.5 micrometers.
Claim 12
Independent Claim
27 words
A method as claimed in any of claims 4 to 10, wherein the second particulate material has an average diameter of from about 0.01 to 1.5 micrometers.
Claim 13
Dependent Claim
88 words
Use of charged, particulate material for aiding the separation of components of a liquid cell culture medium by membrane filtration, characterized in that:- (a) the medium and an effective amount of first charged, particulate material, for example ion exchange resin, are mixed together to form a suspension of matter in the medium, the charged, particulate material having an average diameter of from about 0.01 to about 2.5 micrometers and bearing 0.1 to 1.5 functional groups per monomer unit; and (b) the suspended matter is recovered using membrane filtration.
Claim 14
Dependent Claim
88 words
Use of charged, particulate material for aiding the separation of components of a liquid cell culture medium by membrane filtration, characterized in that:- (a) the medium and an effective amount of first charged, particulate material, for example ion exchange resin, are mixed together to form a suspension of matter in the medium, the charged, particulate material having an average diameter of from about 0.01 to about 2.5 micrometers and bearing 0.1 to 1.5 functional groups per monomer unit; and (b) the suspended matter is recovered using membrane filtration.
References Cited (Backward Citations)
No citation records indexed.
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