EP0245088A2
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EP
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Regenerable support matrix for immobilizing biologically active materials
Assignee: Honeywell UOP LLC
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Filing: 1987-05-06
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Granted: —
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Expires: 2007-05-06
Abstract
Abstract A support matrix suitable for the immobilization of biologically active materials comprises a core support made from a refractory inorganic oxide, glass or ceramic coated with a dialkylaminoalkyl ether of cellulose having groups of the formula R a R b (CH 2 )p, in which R a and R b are alkyl groups having 1 to 20 carbon atoms, and p is an integer from 2 to 10. The matrix can be used to immobilize biologically active material, e.g. proteinaceous material having an isoelectric point greater than 7.0 (e.g. enzymes, cofactors, antibodies or antigens). The matrix can be readily and repeatedly regenerated by washing with an aqueous base or salt solution.
Inventors
- 👤 Chris D. Lapins
- 👤 Yoshihisa Tsuda
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 12:11
CPC Classifications
C; C12; C12M; C12M1/00; C12M1/40; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/12; C12M1/16; C12N11/14
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
76 words
A regenerable support matrix characterized in that it comprises a core support made from a refractory inorganic oxide, glass, or a ceramic material, coated with a dialkylaminoalkyl ether of cellulose, having dialkylaminoalkyl groups of the formula R a R b N(CH 2 )p-, wherein R a , and R b , which may be the same or different, are alkyl groups having 1 to 20 carbon atoms, and p is an integer from 2 to 10.
Claim 2
Dependent Claim
76 words
A regenerable support matrix characterized in that it comprises a core support made from a refractory inorganic oxide, glass, or a ceramic material, coated with a dialkylaminoalkyl ether of cellulose, having dialkylaminoalkyl groups of the formula R a R b N(CH 2 )p-, wherein R a , and R b , which may be the same or different, are alkyl groups having 1 to 20 carbon atoms, and p is an integer from 2 to 10.
Claim 3
Dependent Claim
30 words
A matrix according to Claim 1 characterized in that the refractory inorganic oxide is aluminium oxide, silicon oxide, thorium oxide, zirconium oxide, titanium oxide, magnesium oxide, or a combination thereof.
Claim 4
Dependent Claim
28 words
A matrix according to Claim 1 or 2, characterized in that R a and R b are ethyl groups, and p is an integer from 2 to 4.
Claim 5
Dependent Claim
22 words
A matrix according to any preceding claim characterized in that from 1 to 20% by weight of finished material, calculated as cellulose.
Claim 6
Dependent Claim
31 words
An immobilized biologically active system, characterized in that it comprises a combination of a support matrix according to any one of Claims 1 to 4 and an immobilized biologically active material.
Claim 7
Dependent Claim
38 words
An immobilized biologically active system according to Claim 5 characterized in that the biologically active material is a proteinaceous material, whose isoelectric point is greater than 7.0, selected from an enzyme, a cofactor, an antibody, or an antigen.
Claim 8
Independent Claim
55 words
A method of making a regenerable support matrix according to any of claims 1 to 4 characterized by coating a core support with a cellulose ester, removing by hydrolysis at least 80% of the ester groups to provide free hydroxyl groups, converting said hydroxyl groups into dialkylaminoalkyl ether groups, and recovering the resulting support matrix.
Claim 9
Dependent Claim
54 words
A method according to Claim 7 characterized in that hydrolysis is effected by reacting the cellulose ester on the core support with a solution of an alkali having a concentration from 0.5 to 4 weight-volume percent at a temperature from 25 to 70°C, and in that the hydroxyl groups are converted into dialkylaminoalkyl halide.
Claim 10
Dependent Claim
74 words
A method of regenerating an immobilized biologically active system according to Claim 5 or 6 characterized by contacting the immobilized system with an aqueous solution of a base or highly dissociated salt at conditions selected to remove the spent biologically active material immobilized thereon, removing excess base or salt by washing with water or a buffer solution, recovering the regenerated support matrix, and immobilizing fresh biologically active material on the resulting regenerated support matrix.
References Cited (Backward Citations)
No citation records indexed.
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