EP0248524A2
Expired / Lapsed
EP
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Method of separating and/or purifying bio-affecting substances using particulate, polymeric adsorbents
Assignee: Rohm and Haas Co
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Filing: 1987-04-27
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Granted: —
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Expires: 2007-04-27
Abstract
Abstract A method is provided for (i) separating a first water-soluble or water-dispersible bio-affecting substance, contained in a liquid medium, from one or more other components of the liquid medium, said other components being selected from impurities and other water-soluble and/or water-dispersible bio-affecting substances, and/or (ii) purifying the first bio-affecting substance contained in the liquid medium together with said one or more other components, the method comprising: forming a mixture comprising the liquid medium and particulate polymeric adsorbent, the adsorbent forming a complex with the first bio-affecting substance or with said other component(s); and subjecting the liquid medium, containing the complex, to membrane filtration. The method of the invention enabales the disadvantages and limitations of known separation and purification processes to be overcome or minimised.
Inventors
- 👤 Michael Joe Byers
- 👤 Eric Gilbert Isacoff
- 👤 John Otto Naples
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 01:29
CPC Classifications
C; C02; C02F; C02F1/00; C02F1/28; C02F1/285; B; B01; B01D; B01D15/00; B01D15/02; B01D15/08; B01D15/10; B01D15/18; B01D15/1892; B01D61/00; B01D61/14; B01D61/145; B01D61/147; B01D61/16; B01J; B01J20/00; B01J20/22; B01J20/26; B01J20/28; B01J20/28014; B01J20/28016; B01J20/28019; B01J45/00; B01J47/00; B01J47/015; B01D2311/00; B01D2311/04
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
165 words
A method of (i) separating a first water-soluble or water-dispersible bio-affecting substance, contained in a first liquid medium, from one or more other components of said first liquid medium, said other components being selected from impurities and other water-soluble and/or water-dispersible bio-affecting substances, and/or (ii) purifying said first bio-affecting substance contained in said first liquid medium together with said one or more other components, characterized in that the method comprises:- (a) forming a mixture comprising the first liquid medium and particulate, polymeric adsorbent capable of preferentially adsorbing the first bio-affecting substance or said one or more other components therefrom, the adsorbent having an average particle size of 0.01 to 5 micrometers, whereby the adsorbent reversibly binds the first bio-affecting substance or said one or more other components to form a complex; (b) subjecting the mixture from step (a) to membrane filtration, the membrane being impermeable to said complex, whereby said first bio-affecting substance is separated from said one or more other components and/or is purified.
Claim 2
Independent Claim
165 words
A method of (i) separating a first water-soluble or water-dispersible bio-affecting substance, contained in a first liquid medium, from one or more other components of said first liquid medium, said other components being selected from impurities and other water-soluble and/or water-dispersible bio-affecting substances, and/or (ii) purifying said first bio-affecting substance contained in said first liquid medium together with said one or more other components, characterized in that the method comprises:- (a) forming a mixture comprising the first liquid medium and particulate, polymeric adsorbent capable of preferentially adsorbing the first bio-affecting substance or said one or more other components therefrom, the adsorbent having an average particle size of 0.01 to 5 micrometers, whereby the adsorbent reversibly binds the first bio-affecting substance or said one or more other components to form a complex; (b) subjecting the mixture from step (a) to membrane filtration, the membrane being impermeable to said complex, whereby said first bio-affecting substance is separated from said one or more other components and/or is purified.
Claim 3
Independent Claim
159 words
A method for (i) separating a first water-soluble or water-dispersible bio-affecting substance from a first liquid medium comprising said first bio-affecting substance and one or more of impurities and other water-soluble and/or water-dispersible bio-affecting substances, and/or (ii) purifying said first bio-affecting substance contained in said first liquid medium, characterized in that the method comprises:- (a) forming a mixture comprising the first liquid medium and particulate polymeric adsorbent capable of preferentially adsorbing the first bio-affecting substance therefrom, the adsorbent having an average particle size of 0.01 to 5 micrometers, whereby the adsorbent reversibly binds the first bio-affecting substance to form a complex; (b) subjecting the mixture from step (a), containing the adsorbent/first bio-affecting substance complex, to membrane filtration wherein the membrane is permeable to any of said impurities and other bio-affecting substances present but not to the complex, whereby the complex and any of said impurities and other bio-affecting substances present are separated, and/or said first bio-affecting substance is purified.
Claim 4
Independent Claim
159 words
A method for (i) separating a first water-soluble or water-dispersible bio-affecting substance from a first liquid medium comprising said first bio-affecting substance and one or more of impurities and other water-soluble and/or water-dispersible bio-affecting substances, and/or (ii) purifying said first bio-affecting substance contained in said first liquid medium, characterized in that the method comprises:- (a) forming a mixture comprising the first liquid medium and particulate polymeric adsorbent capable of preferentially adsorbing the first bio-affecting substance therefrom, the adsorbent having an average particle size of 0.01 to 5 micrometers, whereby the adsorbent reversibly binds the first bio-affecting substance to form a complex; (b) subjecting the mixture from step (a), containing the adsorbent/first bio-affecting substance complex, to membrane filtration wherein the membrane is permeable to any of said impurities and other bio-affecting substances present but not to the complex, whereby the complex and any of said impurities and other bio-affecting substances present are separated, and/or said first bio-affecting substance is purified.
Claim 5
Dependent Claim
34 words
A method as claimed in claim 2, comprising the additional step of: (c) liberating the first bio-affecting substance from the polymer particles of the complex, for example with electrolyte, into a second liquid medium.
Claim 6
Dependent Claim
52 words
A method as claimed in claim 3, which comprises the additional step of: (d) subjecting the second liquid medium, containing the liberated first bio-affecting substance and the adsorbent polymer particles, to membrane filtration as in step (b), whereby the first bio-affecting substance, but not the adsorbent polymer particles, accumulates in the permeate.
Claim 7
Independent Claim
27 words
A method as claimed in any preceding claim, wherein the adsorbent comprises ion exchange resin, for example, bearing up to about 1.5 functional groups per monomer unit.
Claim 8
Dependent Claim
22 words
A method as claimed in claim 5, wherein the particles of the ion exchange resin comprise substantially spherical beads or ground particles.
Claim 9
Dependent Claim
53 words
A method as claimed in claim 5 or claim 6, wherein the ion exchange resin comprises acidic or basic ion exchange resin, for example the first bio-affecting substance is positively charged and the ion exchange resin is acidic, or the first bio-affecting substance is negatively charged and the ion exchange resin is basic.
Claim 10
Independent Claim
25 words
A method as claimed in any of claims 1 to 4, wherein the particulate, polymeric adsorbent comprises uncharged polymer, for example, ion exchange resin precursor.
Claim 11
Dependent Claim
19 words
A method as claimed in claim 8, wherein the particulate, polymeric adsorbent and the first bio-affecting substance are hydrophobic.
Claim 12
Independent Claim
27 words
A method as claimed in any preceding claim, wherein the first bio-affecting substance comprises proteinaceous material, an amino acid, an antibiotic, a vitamin or a nucleic acid.
Claim 13
Independent Claim
50 words
A method as claimed in any preceding claim, wherein the first bio-affecting substance and said one or more of impurities and other bio-affecting substances are suspended or dissolved in the first liquid medium, and/or the first liquid medium is aqueous and the particulate, polymeric adsorbent is water-insoluble or substantially water-insoluble.
Claim 14
Independent Claim
34 words
A method as claimed in any preceding claim, wherein the other water-soluble and/or water-dispersible bio-affecting substances comprise one or more of a protein, an amino acid, a nucleic acid, an antibiotic, or a vitamin.
Claim 15
Dependent Claim
171 words
Use of particulate, polymeric adsorbent for aiding (i) separation of a first water-soluble or water-dispersible bio-affecting substance, contained in a first liquid medium, from one or more other components of said first liquid medium, said other components being selected from impurities and other water-soluble and/or water-dispersible bio-affecting substances, and/or (ii) purification of said first bio-affecting substance contained in said first liquid medium together with said one or more other components, characterized in that: (a) a mixture is formed comprising the first liquid medium and particulate, polymeric adsorbent capable of preferentially adsorbing the first bio-affecting substance or said one or more other components therefrom, the adsorbent having an average particle size of 0.01 to 5 micrometers, whereby the adsorbent reversibly binds the first bio-affecting substance or said one or more other components to form a complex; and (b) the mixture from step (a) is subjected to membrane filtration, the membrane being impermeable to said complex, whereby said first bio-affecting substance is separated from said one or more other components and/or is purified.
Claim 16
Dependent Claim
171 words
Use of particulate, polymeric adsorbent for aiding (i) separation of a first water-soluble or water-dispersible bio-affecting substance, contained in a first liquid medium, from one or more other components of said first liquid medium, said other components being selected from impurities and other water-soluble and/or water-dispersible bio-affecting substances, and/or (ii) purification of said first bio-affecting substance contained in said first liquid medium together with said one or more other components, characterized in that: (a) a mixture is formed comprising the first liquid medium and particulate, polymeric adsorbent capable of preferentially adsorbing the first bio-affecting substance or said one or more other components therefrom, the adsorbent having an average particle size of 0.01 to 5 micrometers, whereby the adsorbent reversibly binds the first bio-affecting substance or said one or more other components to form a complex; and (b) the mixture from step (a) is subjected to membrane filtration, the membrane being impermeable to said complex, whereby said first bio-affecting substance is separated from said one or more other components and/or is purified.
References Cited (Backward Citations)
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