EP0251283A2
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EP
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Poly-labelled oligonucleotide derivative
Assignee: Wakunaga Pharmaceutical Co Ltd
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Filing: 1987-06-29
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Granted: —
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Expires: 2007-06-29
Abstract
Abstract A poly-labelled oligonucleotide having a plurality of labels, comprising labels carried on a polylysine and having the polylysine introduced on the extension from 5'-and/or 3'-end through phosphate group is useful as a probe for hybridization.
Inventors
- 👤 Akio Wakunaga Seiyaku Kabushiki Kaisha Yamane
- 👤 Tasuro Wakunaga Seiyaku Kabushiki Kaisha Kawasoe
- 👤 Noriko Wakunaga Seiyaku Kabushiki Kaisha Tsukumo
- 👤 Kenichi Wakunaga Seiyaku Kabushiki Kaisha Miyoshi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 14:15
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
112 words
A poly-labelled oligonucleotide derivative represented by the following formula [1]: wherein: either one of R' and R 2 is a group represented by the formula [II] shown below and the other is hydrogen atom or phosphate residue, or both of them are groups represented by the formula [II] shown below; n is any desired natural number; and B is a base constituting the nucleotide: wherein: a and b are any desired natural numbers with a relationship of b + 1 ≥ a; R 3 is a divalent straight or branched hydrocarbon residue; X represents oxygen atom or imino group; Lys represents lysine residue; and Y is a label covalently bonded to Lys.
Claim 2
Dependent Claim
112 words
A poly-labelled oligonucleotide derivative represented by the following formula [1]: wherein: either one of R' and R 2 is a group represented by the formula [II] shown below and the other is hydrogen atom or phosphate residue, or both of them are groups represented by the formula [II] shown below; n is any desired natural number; and B is a base constituting the nucleotide: wherein: a and b are any desired natural numbers with a relationship of b + 1 ≥ a; R 3 is a divalent straight or branched hydrocarbon residue; X represents oxygen atom or imino group; Lys represents lysine residue; and Y is a label covalently bonded to Lys.
Claim 3
Dependent Claim
13 words
A poly-labelled oligonucleotide according to claim 1, wherein n is 6 to 200.
Claim 4
Dependent Claim
13 words
A poly-labelled oligonucleotide according to claim 2, wherein n is 10 to 60.
Claim 5
Dependent Claim
13 words
A poly-labelled oligonucleotide according to claim 3, wherein n is 15 to 40.
Claim 6
Dependent Claim
17 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein a is 2 to 50.
Claim 7
Dependent Claim
13 words
A poly-labelled oligonucleotide according to claim 5, wherein a is 2 to 21.
Claim 8
Dependent Claim
13 words
A poly-labelled oligonucleotide according to claim 6, wherein a is 2 to 11.
Claim 9
Dependent Claim
17 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein b is 1 to 50.
Claim 10
Dependent Claim
13 words
A poly-labelled oligonucleotide according to claim 8, wherein b is 1 to 20.
Claim 11
Dependent Claim
14 words
A poly-labelled oligonucleotide according to claim 9 , wherein b is 1 to 10.
Claim 12
Dependent Claim
17 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein b is at least 2.
Claim 13
Dependent Claim
25 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein both of R I and R 2 are represented by the formula [II].
Claim 14
Dependent Claim
35 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 - 11, wherein one of R' and R 2 is a group represented by the formula [II] and the other is hydrogen atom.
Claim 15
Dependent Claim
36 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 - 11, wherein one of R I and R 2 is a group represented by the formula [II] and the other is phosphate residue.
Claim 16
Dependent Claim
16 words
A poly-labelled oligonucleotide according to any one of the preceding claims. wherein X is oxygen atom.
Claim 17
Dependent Claim
19 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 - 14, wherein X is imino group.
Claim 18
Dependent Claim
26 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein the R 3 group is an alkylene group having 2 to 20 carbon atoms.
Claim 19
Dependent Claim
20 words
A poly-labelled oligonucleotide according to claim 17, wherein R 3 is an alkylene group having 2 to 6 carbon atoms.
Claim 20
Dependent Claim
16 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein Y is biotin residue.
Claim 21
Dependent Claim
18 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 18, wherein Y is 2,4-dinitrophenyl group.
Claim 22
Dependent Claim
24 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 - 18, wherein Y is fluoresceine residue or a fluoresceine derivative residue.
Claim 23
Dependent Claim
14 words
A poly-labelled oligonucleotide according to claim 21, wherein the fluoresceine derivative is fluoresceine isothiocyanate.
Claim 24
Dependent Claim
24 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 - 18, wherein Y is rhodamine residue or a rhodamine derivative residue.
Claim 25
Dependent Claim
17 words
A poly-labelled oligonucleotide according to claim 23, wherein the rhodamine derivative is tetramethylrhodamine isothiocyanate or Texas Red.
Claim 26
Dependent Claim
18 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 - 18, wherein Y is 4-fluoro7-nitrobenzofuran.
Claim 27
Dependent Claim
18 words
A poly-labelled oligonucleotide according to any one of the preceding claims 1 - 18, wherein Y is dansyl.
Claim 28
Dependent Claim
19 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein B is adenine, thymine, cytosine or guanine.
Claim 29
Dependent Claim
17 words
A poly-labelled oligonucleotide according to any one of the preceding claims, wherein B is protected by acylation.
References Cited (Backward Citations)
No citation records indexed.
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