Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
88 words
1 . A method for detecting a specific protein (or peptide) characterized by reacting a test sample containing said protein (or peptide), from which obstructive substances have been removed by filtration, with one or more monoclonal antibodies or polyclonal antibodies specific for said protein (or peptide) and detecting the result of the antigen-antibody reaction with a detecting agent comprising a conjugate of colloidal metal particles and of different monoclonal antibodies having a high specificity against a plurality of mutually different epitopes (antigen determinants) of said protein (or peptide).
Claim 2
Dependent Claim
88 words
1 . A method for detecting a specific protein (or peptide) characterized by reacting a test sample containing said protein (or peptide), from which obstructive substances have been removed by filtration, with one or more monoclonal antibodies or polyclonal antibodies specific for said protein (or peptide) and detecting the result of the antigen-antibody reaction with a detecting agent comprising a conjugate of colloidal metal particles and of different monoclonal antibodies having a high specificity against a plurality of mutually different epitopes (antigen determinants) of said protein (or peptide).
Claim 3
Dependent Claim
45 words
The method according to claim 1, characterized by contacting the test sample with an absorptive carrier to which the monoclonal antibodies or polyclonal antibodies specific for said protein (or peptide) are fixed and thereafter treating the absorptive carrier with a solution of said detecting agent.
Claim 4
Dependent Claim
21 words
The method according to claim 1 or 2, characterized by prefiltering the test sample with a filter coated with skim milk.
Claim 5
Dependent Claim
58 words
A reagent for carrying out the method according to claims 1 to 3, characterized by an absorptive carrier impregnated with a buffer solution containing said one or more monoclonal antibodies or polyclonal antibodies specific for said protein (or peptide), dried and treated with a blocking agent, such as skim milk, on the areas not covered with said antibodies.
Claim 6
Dependent Claim
23 words
The reagent according to claim 4 characterized in that the absorptive carrier is a test piece of paper or a nylon membrane filter.
Claim 7
Dependent Claim
55 words
A detecting agent for carrying out the method according to claims 1 to 3, characterized by comprising colloidal particles bonded with monoclonal antibodies having a high specificity against a plurality of mutually different epitopes (antigen determinants) of said protein (or peptide) and different from those present on the absorptive carrier as defined in claim 4.
Claim 8
Dependent Claim
22 words
The detecting agent according to claim 6 characterized by comprising a solution or the freeze-dried product, which if desired contains a buffer.
Claim 9
Dependent Claim
22 words
A reagent kit comprising the reagent according to claims 4 and 5 and the detecting agent according to claims 6 and 7.
Claim 10
Dependent Claim
29 words
A filtration apparatus for the pretreatment of the test sample used in the method according to claims 1 to 3, characterized by a filter coated with skim milk etc.