EP0259891A2
Expired / Lapsed
EP
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[Leu 13] motilin, DNAs coding for same and methods for producing same
Assignee: KH Neochem Co Ltd
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Filing: 1987-09-11
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Granted: —
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Expires: 2007-09-11
Abstract
Abstract A novel motilin analog peptide containing leucine instead of the usual l3th amino acid methionine, as in the naturally occurring motilin, is produced by gene recombination techniques in which several or identical genes, each coding for the novel peptide, are joined in series into a vector. The resultant recombinant DNA is introduced into Escherichia coli and the resultant transformant is cultivated. The resulting polymeric peptide can be cleaved to give the desired peptide.
Inventors
- 👤 Shinkichi Honda
- 👤 Tatsunari Nishi
- 👤 Seiga Itoh
- 👤 Moriyuki Sato
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 05:20
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; A; A61; A61P; A61P1/00; A61P1/12; C07K14/63; C07K5/00; C07K5/04; C07K5/10; C07K5/1019; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; A61K; A61K38/00; C07K2319/00; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
8 words
A novel peptide having the amino acid sequence:
Claim 2
Dependent Claim
8 words
A novel peptide having the amino acid sequence:
Claim 3
Dependent Claim
11 words
A DNA coding for a peptide having the amino acid sequence:
Claim 4
Dependent Claim
11 words
A DNA coding for a peptide having the amino acid sequence:
Claim 5
Dependent Claim
56 words
A DNA having the nucleotide sequence: wherein the lead lines directed to the symbols Cla I, Bgl II, Bam HI and Sac I indicate the sites of cleavage by the respective restriction enzymes represented by said symbols and wherein A, T, G and C represent the bases adenine, thymine, guanine and cytosine in the nucleotide, respectively.
Claim 6
Dependent Claim
56 words
A DNA having the nucleotide sequence: wherein the lead lines directed to the symbols Cla I, Bgl II, Bam HI and Sac I indicate the sites of cleavage by the respective restriction enzymes represented by said symbols and wherein A, T, G and C represent the bases adenine, thymine, guanine and cytosine in the nucleotide, respectively.
Claim 7
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ CGATCAGATCTTCATGTTCGTTCCGATTTTCACTTACGGTGAACTGCAAC 3′ (Formula 3)
Claim 8
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ CGATCAGATCTTCATGTTCGTTCCGATTTTCACTTACGGTGAACTGCAAC 3′ (Formula 3)
Claim 9
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ AGTTCACCGTAAGTGAAAATCGGAACGAACATGAAGATCTGAT 3′ (Formula 4)
Claim 10
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ AGTTCACCGTAAGTGAAAATCGGAACGAACATGAAGATCTGAT 3′ (Formula 4)
Claim 11
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ GTCTGCAAGAGAAAGAACGTAACAAAGGTCAGCGGATCCTGTAAGAGCT 3ʹ (Formula 5)
Claim 12
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ GTCTGCAAGAGAAAGAACGTAACAAAGGTCAGCGGATCCTGTAAGAGCT 3ʹ (Formula 5)
Claim 13
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ CTTACAGGATCCGCTGACCTTTGTTACGTTCTTTCTCTTGCAGACGTTGC 3ʹ (Formula 6)
Claim 14
Dependent Claim
11 words
A DNA having the nucleotide sequence: 5′ CTTACAGGATCCGCTGACCTTTGTTACGTTCTTTCTCTTGCAGACGTTGC 3ʹ (Formula 6)
Claim 15
Dependent Claim
21 words
A recombinant DNA having inserted therein a DNA coding for the peptide having the amino acid sequence defined by claim l.
Claim 16
Independent Claim
71 words
A method of producing a peptide having the amino acid sequence of claim l which comprises (a) cultivating, in a nutrient growth medium, a microorganism harboring a recombinant DNA with a DNA coding for a peptide having the amino acid sequence of claim l inserted therein and allowing the peptide having the amino acid sequence of claim l to accumulate in the culture, and (b) harvesting said peptide from said culture.
Claim 17
Dependent Claim
9 words
l0. A novel peptide having the amino acid sequence:
Claim 18
Dependent Claim
9 words
l0. A novel peptide having the amino acid sequence:
Claim 19
Independent Claim
33 words
A method of producing a peptide having the amino acid sequence of claim l, which comprises degrading the peptide having the amino acid sequence of claim l0 with carboxypeptidase A and carboxypeptidase B.
Claim 20
Dependent Claim
45 words
A novel polymeric peptide having the amino acid sequence: [Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Gly Gln Arg Ile phe Met] n wherein n has a value of 2 to 32.
Claim 21
Dependent Claim
45 words
A novel polymeric peptide having the amino acid sequence: [Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Gly Gln Arg Ile phe Met] n wherein n has a value of 2 to 32.
Claim 22
Dependent Claim
42 words
A spacer peptide having the amino acid sequence X-Arg-Ile-Phe Met-X in which X is a neighboring peptide and Met is methionine, the spacer peptide being removable by treatment in sequence with cyanogen bromide, carboxypeptidase A and carboxypeptidase B to yield monomeric X.
Claim 23
Dependent Claim
42 words
A spacer peptide having the amino acid sequence X-Arg-Ile-Phe Met-X in which X is a neighboring peptide and Met is methionine, the spacer peptide being removable by treatment in sequence with cyanogen bromide, carboxypeptidase A and carboxypeptidase B to yield monomeric X.
Claim 24
Dependent Claim
17 words
The spacer peptide of claim l3, in which X is the amino acid sequence of claim l.
Claim 25
Dependent Claim
22 words
A pharmaceutical composition containing a therapeutically effective amount of the peptide of claim l together with a pharmaceutically acceptable carrier or diluent.
References Cited (Backward Citations)
No citation records indexed.
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