EP0271289A2
Expired / Lapsed
EP
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Method for immobilization of enzyme and immobilized enzymes
Assignee: Hitachi Ltd
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Filing: 1987-12-03
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Granted: —
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Expires: 2007-12-03
Abstract
Abstract An immobilization enzyme having high activity and stability can be obtained by immobilizing an enzyme on a water-insoluble amino group-containing carrier such as silica gel, porous glass or the like having amino groups introduced thereinto, by use of a polyfunctional cross-linking agent in the presence of a phenolic carboxylic acid having one or more hydroxyl groups such as tannic acid or the like or in the presence of a phenolic carboxylic acid having one or more hydroxyl groups and chitosan.
Inventors
- 👤 Yuusaku Nishimura
- 👤 Masahiko Ishida
- 👤 Ryoichi Haga
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 11:29
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/06; C12N11/10; C12N11/14
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
63 words
A method for immobilization of enzyme which comprises immobilizing an enzyme in an aqueous solution containing the enzyme on a water-insoluble carrier containing amino groups, said immobilization being carried out by covalent binding reaction of the enzyme with the carrier through a polyfunctional crosslinking agent characterized by the presence of a phenolic carboxylic acid having one or more hydroxyl groups during said reaction.
Claim 2
Independent Claim
63 words
A method for immobilization of enzyme which comprises immobilizing an enzyme in an aqueous solution containing the enzyme on a water-insoluble carrier containing amino groups, said immobilization being carried out by covalent binding reaction of the enzyme with the carrier through a polyfunctional crosslinking agent characterized by the presence of a phenolic carboxylic acid having one or more hydroxyl groups during said reaction.
Claim 3
Dependent Claim
26 words
A method according to claim 1 wherein the water-insoluble carrier containing amino groups is aminated silica gel, aminated porous glass, aminated zeolite, or water-insoluble cross-linked chitosan.
Claim 4
Dependent Claim
17 words
A method according to claim 1 or claim 2 wherein the polyfunctional crosslinking agent is a polyaldehyde.
Claim 5
Dependent Claim
11 words
A method according to claim 3 wherein the polyaldehyde is glutaraldehyde.
Claim 6
Independent Claim
23 words
A method according to any one of claims 1 to 4 wherein the phenolic carboxylic acid is tannic acid, gallic acid or catechol.
Claim 7
Dependent Claim
14 words
A method according to claim 5, wherein the phenolic carboyxlic acid is tannic acid.
Claim 8
Independent Claim
26 words
A method according to any one of claims 1 to 6 wherein a basic polysaccharide having high adsorbability for enzymes is also present during said reaction.
Claim 9
Dependent Claim
17 words
A method according to claim 7 wherein the basic polysaccharide having high adsorbability for enzymes is chitosan.
Claim 10
Independent Claim
33 words
A method according to any one of claims 1 to 8 which comprises forming Schiff bases between the water-insoluble carrier containing amino groups and the enzyme by use of said polyfunctional crosslinking agent.
Claim 11
Dependent Claim
63 words
An immobilized enzyme obtained by immobilization on a water-insoluble carrier by use of a polyfunctional crosslinking agent, said immobilization being carried out by adsorption of an enzyme on a phenolic carboxylic acid having one or more hydroxyl groups, followed by covalent binding reaction of the functional groups of the crosslinking agent with the amino groups of the water-insoluble carrier and of the enzyme.
Claim 12
Dependent Claim
63 words
An immobilized enzyme obtained by immobilization on a water-insoluble carrier by use of a polyfunctional crosslinking agent, said immobilization being carried out by adsorption of an enzyme on a phenolic carboxylic acid having one or more hydroxyl groups, followed by covalent binding reaction of the functional groups of the crosslinking agent with the amino groups of the water-insoluble carrier and of the enzyme.
References Cited (Backward Citations)
No citation records indexed.
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