EP0276152A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Orthographic flow immunoassays and devices
Assignee: Synbiotics LLC
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Filing: 1988-01-21
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Granted: —
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Expires: 2008-01-21
Abstract
Abstract Methods and composition are provided for diagnostic assays, where a sample traverses a bibulous matrix in a first plane and the flow path redirected into a second plane at a binding site, where a signal is created in relation to the presence of analyte. Various protocols may be employed where components of a signal producing system may be combined with the sample, the matrix, or added at the binding site.
Inventors
- 👤 Patrick F. Coleman
- 👤 Edward T. Maggio
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 12:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N33/54389; Y; Y10; Y10S; Y10S435/00; Y10S435/97
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
90 words
A device for the detection of an analyte comprising: a matrix in a first plane providing for capillary transport of an aqueous sample medium; a first port for directing sample medium to a first site on said matrix; means in contact with said matrix for directing flow of said medium at a second site distant from said first site in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to said second site; and absorbant means for receiving said medium in said second plane.
Claim 2
Independent Claim
90 words
A device for the detection of an analyte comprising: a matrix in a first plane providing for capillary transport of an aqueous sample medium; a first port for directing sample medium to a first site on said matrix; means in contact with said matrix for directing flow of said medium at a second site distant from said first site in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to said second site; and absorbant means for receiving said medium in said second plane.
Claim 3
Dependent Claim
25 words
A device according to Claim 1, including a second port situated over said second site for directing fluids through said second site to said absorbant.
Claim 4
Dependent Claim
28 words
A device according to Claim 1 or Claim 2, including means in contact with said matrix for linearly directing medium from said first site to said second site.
Claim 5
Dependent Claim
28 words
A device according to Claim 1, Claim 2 or Claim 3, including means for urging said matrix and absorbant toward each other in order to maintain intimate contact.
Claim 6
Independent Claim
112 words
A device for the detection of at least one analyte using a single sample, said device comprising: a plurality of matrixes in a first plane meeting at a central hub providing for capillary transport of an aqueous sample medium; a first port for directing sample medium to said hub of said matrix; means in contact with each of said matrixes for directing flow of said medium at a second site on each of said matrixes distant from said hub in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to each of said second sites; and absorbant means for receiving said medium in said second plane.
Claim 7
Independent Claim
112 words
A device for the detection of at least one analyte using a single sample, said device comprising: a plurality of matrixes in a first plane meeting at a central hub providing for capillary transport of an aqueous sample medium; a first port for directing sample medium to said hub of said matrix; means in contact with each of said matrixes for directing flow of said medium at a second site on each of said matrixes distant from said hub in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to each of said second sites; and absorbant means for receiving said medium in said second plane.
Claim 8
Dependent Claim
112 words
A multiunit device for the detection of at least one analyte comprising a plurality of containers, each container sharing at least one common wall with an adjacent container, each container housing: a matrix in a first plane providing for capillary transport of an aqueous sample medium; a first port for directing sample medium to a first site on said matrix; means in contact with said matrix for directing flow of said medium at a second site distant from said first site in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to said second site; and absorbant means for receiving said medium in said second plane.
Claim 9
Dependent Claim
112 words
A multiunit device for the detection of at least one analyte comprising a plurality of containers, each container sharing at least one common wall with an adjacent container, each container housing: a matrix in a first plane providing for capillary transport of an aqueous sample medium; a first port for directing sample medium to a first site on said matrix; means in contact with said matrix for directing flow of said medium at a second site distant from said first site in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to said second site; and absorbant means for receiving said medium in said second plane.
Claim 10
Independent Claim
111 words
A device for the detection of an analyte comprising: a container including a cover; a bibulous matrix in a first plane providing for capillary transport of an aqueous sample medium in close juxtaposition to said cover; a first port in said cover for directing sample medium to a first site on said matrix; means in contact with said matrix for directing flow of said medium at a second site distant from said first site in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to said second site; and absorbant means in close juxtaposition to said matrix for receiving said medium in said second plane.
Claim 11
Independent Claim
111 words
A device for the detection of an analyte comprising: a container including a cover; a bibulous matrix in a first plane providing for capillary transport of an aqueous sample medium in close juxtaposition to said cover; a first port in said cover for directing sample medium to a first site on said matrix; means in contact with said matrix for directing flow of said medium at a second site distant from said first site in a second plane substantially orthogonal to said first plane; a specific binding pair member bound to said second site; and absorbant means in close juxtaposition to said matrix for receiving said medium in said second plane.
Claim 12
Dependent Claim
15 words
A device according to Claim 7, wherein said specific binding pair member is an immunoglobulin.
Claim 13
Independent Claim
120 words
A method for determining an analyte in a sample, said method comprising: contacting at a first site a matrix in a first plane capable of transporting an aqueous medium by capillary transport with an aqueous sample medium; directing said sample medium through said matrix toward a second site distant from said first site, wherein a specific binding pair member is bound at said site; directing said sample medium through said second site in a plane substantially orthogonal to the plane of said matrix, where a complex is formed between said specific binding pair member and its homologous binding pair member; and determining the presence of complex formation at said second site as indicating the presence of analyte in said sample.
Claim 14
Independent Claim
120 words
A method for determining an analyte in a sample, said method comprising: contacting at a first site a matrix in a first plane capable of transporting an aqueous medium by capillary transport with an aqueous sample medium; directing said sample medium through said matrix toward a second site distant from said first site, wherein a specific binding pair member is bound at said site; directing said sample medium through said second site in a plane substantially orthogonal to the plane of said matrix, where a complex is formed between said specific binding pair member and its homologous binding pair member; and determining the presence of complex formation at said second site as indicating the presence of analyte in said sample.
Claim 15
Dependent Claim
57 words
A method according to Claim 9, wherein said determining comprises passing a solution of an enzyme conjugate capable of binding directly or indirectly with said analyte through said second site, followed by passing a solution of substrate for said enzyme through said second site, whereby said enzyme converts said substrate to a product providing a visual signal.
Claim 16
Dependent Claim
58 words
A method according to Claim 9, wherein said second site has anti-enzyme bound to said matrix, and between said first and second sites is a first zone of anti-analyte or analyte analog bound to said matrix, and including the additional step of adding analyte analog-enzyme conjugate to said sample medium before said sample medium contacts said first zone.
References Cited (Backward Citations)
No citation records indexed.
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