EP0301600A2
Expired / Lapsed
EP
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Method for detection of the presence of undesired microorganisms
Assignee: Sumitomo Electric Industries Ltd
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Filing: 1988-08-01
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Granted: —
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Expires: 2008-08-01
Abstract
Abstract A method of detecting undesired microorganisms when cells are cultured in a light permeable container is disclosed. TV camera monitors the image of a well containing microorganisms through a microscope. The output of the TV camera is processed by a picture digital processor so as to count the number of cultured living cells. On the other hand, change of the light absorption of the visible light by the cultured solution is measured. Then the number of undesired microorganisms can be calculated by the number of the living cells and change of the light absorption.
Inventors
- 👤 Shinichi C/O Osaka Works Of Miyake
- 👤 Fumihiko C/O Osaka Works Of Yonemori
- 👤 Masayuki C/O Osaka Works Of Kometani
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 23:04
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12M; C12M41/00; C12M41/26; C12M41/30; C12M41/36; G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
77 words
A method of detecting undesired microorganisms when cells are cultured in a light permeable container, comprising a step of counting the number of cultured living cells appearing in a digital cultured cell picture which is obtained by a picture processing device for processing picture signals fed from a television camera coupled to a observation means provided for observing the container, and a step of measuring change of the absorption of the visible light by the cultured solution.
Claim 2
Independent Claim
77 words
A method of detecting undesired microorganisms when cells are cultured in a light permeable container, comprising a step of counting the number of cultured living cells appearing in a digital cultured cell picture which is obtained by a picture processing device for processing picture signals fed from a television camera coupled to a observation means provided for observing the container, and a step of measuring change of the absorption of the visible light by the cultured solution.
Claim 3
Dependent Claim
40 words
The method according to claim 1, wherein it is judged when the number of the cultured living cells is small and the visible light absorption is greatly increased compared to the light absorption at the last measurement in the past.
Claim 4
Dependent Claim
23 words
The method according to claim 2, the visible light is such light that is not absorbed by indicator contained in the culture solution.
Claim 5
Dependent Claim
37 words
The method according to claim 1, wherein it is judged when the number of the cultured living cells is small and pH value of the culture solution is greatly decreased compared to the last measured pH value.
Claim 6
Dependent Claim
23 words
The method according to claim 4, wherein pH value is calculated from the change of the visible light absorption in the culture solution.
Claim 7
Dependent Claim
64 words
The method according to claim 5, wherein the change of the visible light absorption is change of the light absorption of more than two kinds of light one of which is the light absorption by the light absorbable in the indicator contained in the culture solution and another of which is the light absorption by the light which is not absorbed in the indicator.
Claim 8
Dependent Claim
43 words
The method according to claim 5, change of the visible light absorption includes more than two kinds of change thereof and at least one kind of the change thereof is caused by the light absorption by phenol red contained in the culture solution.
Claim 9
Independent Claim
144 words
A method of detecting undesired microorganisms comprising: a step of radiating at least one kind light to a light permeable container which contains organisms; a step of monitoring image of the microorganisms in the container by receiving the light passed through said container by a television camera attached to a microscope; a step of converting the image information obtained by said camera into digital information representing the picture of cultured living cell contained in said container; a step of counting the number of the living cells; a fifth step of detecting change of light absorption of the light passed through said container; and a step of judging whether the count value of the living cells is smaller than a predetermined value and the change of the light absorption is greater than a further predetermined value for detecting presence of undesired microorganisms in said container.
Claim 10
Independent Claim
144 words
A method of detecting undesired microorganisms comprising: a step of radiating at least one kind light to a light permeable container which contains organisms; a step of monitoring image of the microorganisms in the container by receiving the light passed through said container by a television camera attached to a microscope; a step of converting the image information obtained by said camera into digital information representing the picture of cultured living cell contained in said container; a step of counting the number of the living cells; a fifth step of detecting change of light absorption of the light passed through said container; and a step of judging whether the count value of the living cells is smaller than a predetermined value and the change of the light absorption is greater than a further predetermined value for detecting presence of undesired microorganisms in said container.
References Cited (Backward Citations)
No citation records indexed.
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