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EP0325710A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Reagent and method for measuring leukocytes and hemoglobin in blood

Assignee: Sysmex Corp
•
Filing: 1988-11-04
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Granted: —
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Expires: 2008-11-04
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Abstract

Abstract A reagent for measuring leukocytes and hemoglobin in blood which is a water-soluble mixture containing: (a) a polyoxyethylene based nonionic surfactant repre­sented by the general formula: R₁R₂ CH₂CH₂O) n H (where R₁ is an alkyl, alkenyl or alkynyl group having 6 - 24 carbon atoms; R₂ is -O-, or -COO; and n is an integer of 6 - 50); and (b) a buffer agent that adjusts the pH of an aqueous solution to be within the range of 3 - 11 is disclosed. Measurements of leukocytes and hemoblogin in blood samples are of extreme importance to clinical diagnosis of such diseases as leukemia and anemia. Measurements of eosinophils which are in the category of leukocytes are important to the diagnosis of allergic diseases.

Inventors

  • 👤 Yukio Hamaguchi
  • 👤 Yukio Tsujino

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-08 15:47
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/5005; G01N33/5094; Y; Y10; Y10S; Y10S516/00; Y10S516/01; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666

Claims Analysis

Total Claims: 4
Claim 1
Independent Claim 81 words
A reagent for measuring leukocytes and hemoglobin in blood which is a water-soluble mixture containing: (a) a polyoxyethylene based nonionic surfactant repre­sented by the general formula: R₁-R₂ CH₂CH₂O) n -H (where R₁ is an alkyl, alkenyl or alkynyl group having 6 - 24 carbon atoms; R₂ is -0-, or -COO; and n is an integer of 6 - 50); and (b) a buffer agent that adjusts the pH of an aqueous solution to be within the range of 3 - 11.
Claim 2
Dependent Claim 81 words
A reagent for measuring leukocytes and hemoglobin in blood which is a water-soluble mixture containing: (a) a polyoxyethylene based nonionic surfactant repre­sented by the general formula: R₁-R₂ CH₂CH₂O) n -H (where R₁ is an alkyl, alkenyl or alkynyl group having 6 - 24 carbon atoms; R₂ is -0-, or -COO; and n is an integer of 6 - 50); and (b) a buffer agent that adjusts the pH of an aqueous solution to be within the range of 3 - 11.
Claim 3
Dependent Claim 28 words
A reagent according to Claim 1 which contains a buffer agent that adjusts the pH of an aqueous solution to be within the range of 5 - 11.
Claim 4
Dependent Claim 29 words
A reagent according to Claim 1 wherein n is an integer of l0 - 30 and R₁ is an alkyl, alkenyl or alkynyl having l0 - 20 carbon atoms.

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