EP0337625A2
Expired / Lapsed
EP
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Probes
Assignee: Imperial Chemical Industries Ltd
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Filing: 1989-03-28
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Granted: —
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Expires: 2009-03-28
Abstract
Abstract Process for producing plant hypervariable DNA probes for characterising plants by using Jeffreys probes to isolate homologous sequences in plants, which are then cloned for use as probes. Also claims the probes so obtained, and their sequences, as well as their use to characterise plant material.
Inventors
- 👤 Stephen Robert Barnes
- 👤 Anthony John Stefan Chojecki
- 👤 Allan Daly
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 06:50
CPC Classifications
C; C12; C12N; C12N15/00; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/6895; C12Q2600/00; C12Q2600/156
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
54 words
A process for obtaining hypervariable DNA probes useful in characterising plant material which comprises preparing purified DNA from a target plant species; cutting the purified DNA into fragments; cloning the fragments; screening the cloned fragments with a Jeffreys probe and selecting one or more clones binding to said probe; and multiplying such selected clones.
Claim 2
Dependent Claim
54 words
A process for obtaining hypervariable DNA probes useful in characterising plant material which comprises preparing purified DNA from a target plant species; cutting the purified DNA into fragments; cloning the fragments; screening the cloned fragments with a Jeffreys probe and selecting one or more clones binding to said probe; and multiplying such selected clones.
Claim 3
Dependent Claim
16 words
A process as claimed in claim 1 wherein the target plant species is a crop species.
Claim 4
Dependent Claim
14 words
A process as claimed in claim 2 in which the crop species is dicotyledonous.
Claim 5
Dependent Claim
14 words
A process as claimed in claim 2 in which the crop species is monocotyledonous.
Claim 6
Dependent Claim
14 words
A process as claimed in claim 4 in which the crop is a cereal.
Claim 7
Dependent Claim
17 words
A process as claimed in claim 5 in which the cereal is maize ( Zea mays ).
Claim 8
Dependent Claim
17 words
A process as claimed in claim 5 in which the cereal is rice ( Oryza sativa ).
Claim 9
Dependent Claim
17 words
A process as claimed in any of claims 1-7 wherein the Jeffreys probe is 33.6 or 33.15.
Claim 10
Dependent Claim
21 words
Hypervariable DNA clones useful in the characterisation of plant material and obtained by a process claimed in any of claims 1-8.
Claim 11
Dependent Claim
16 words
Hypervariable DNA clones useful in the characterisation of plant material having the repeat tandem sequence (AGAGGGCGGCGG).
Claim 12
Dependent Claim
16 words
Hypervariable DNA clones useful in the characterisation of plant material having the repeat tandem sequence (AGAGGGCGGCGG).
Claim 13
Dependent Claim
31 words
Hypervariable DNA clones useful in the characterisation of plant material and having repeat tandem sequences at least 70% homologous with the repeat tandem sequences of the clones claimed in claim 10.
Claim 14
Dependent Claim
86 words
Process for characterising plant material which comprises preparing purified DNA from test plant material: cutting the purified DNA with a selected restriction enzyme; separating the cut DNA into an array of fragments by a standard separation technique; probing the array of fragments with a hypervariable probe claimed in any of claims 9-11 and 16; visualising the probe-fragment hybridisations and comparing the pattern so obtained with a corresponding pattern obtained from characterised plant material using the same restriction enzyme and the same probe under the same conditions.
Claim 15
Dependent Claim
24 words
Process as claimed in claim 12 wherein the hypervariable probe is derived from the same plant genus or species as the test plant material.
Claim 16
Dependent Claim
15 words
Process as claimed in claim 13 in which the plant species is Zea mays .
Claim 17
Dependent Claim
15 words
Process as claimed in claim 13 in which the plant species is Oryza sativa .
Claim 18
Dependent Claim
60 words
A process for obtaining hypervariable DNA probes useful in characterising plant material which comprises: preparing purified DNA from a target plant species; cutting the purified DNA into fragments; cloning the fragments; screening the cloned fragments with a hypervariable probe claimed in any of claims 9-11 and selecting one or more clones binding to said probe; and multiplying such selected clones.
Claim 19
Dependent Claim
14 words
A process claimed in claim 16 wherein the hypervariable DNA probe obtained is locus-specific.
References Cited (Backward Citations)
No citation records indexed.
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