Cell analyzer

Claim 2

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to a plurality of parameterts; cell population subdivide means for detecting minimal points in histograms of cell light information obtained by said cell light information detect means with resepect to one or more paramters so as to subdivide a population of the cells in the cell float fluid based on the minimal points; cell light informtion collect means for collecting, based on one or more fractions of the fractions produced by said cell population subdivide means, cell light information of an objective cell population from the cell light information attained by said cell light information detect means; cell light information processing means for processing the cell light information of the objective cell population collected by said cell light informtion collect means; output means for outputting results of the processing conducted by said cell light information processing means; and parameter convert means for converting said one or more parameters depending on a predetermined conversion expression, said cell population subdivide means detecting minimal points from histograms of the cell light information with respect to new parameters attained through a conversion achieved by said parameter convert means.

Claim 3

A cell analyze apparatus according to Claim 1 wherein: said cell light information collect means includes a cell light information separate means, said cell light information separate means separating, from the cell light information detected by said cell light information detect means, cell light information of a cell population belonging to one or more fractions with respect to a paramter, said cell light information collect means collecting, from the separated cell light information, cell light information of a cell population belonging to a predetermined fraction with respect to other parameters.

Claim 4

A cell analyze apparatus according to Claim 1 further including: maximum frequency point extract means for processing the cell light information of the objective cell population collected by said cell light information collect means so as to extract a maximum frequency point on a cytogram of parameters associated with the fraction of the cell population; direct line generate means for radially drawing direct lines from the maximum frequency point extracted by said maximum frequency point extract means on the cytogram; boundary point extract means for extracting boundary points based on histograms on the respective direct lines produced by said direct line generate means; and second cell light information collect means for linking the boundary points extracted by said boundary point extract means to form a final fraction so as to collect cell light information of an objective cell population based on the final fraction.

Claim 5

A cell analyze apparatus according to Claim 1 further including: histogram generate means for processing the cell light information collected by said cell light information collect means so as to attain, for each predetermined frequency of a parameter, a histogram of another parameter; boundary point extract means for extracting boundary points based on each histogram generated by said histogram generate means; and second cell light information collect means for linking the boundary points extracted by said boundary point extract means to form a final fraction so as to collect cell light information of an objective cell population based on the final fraction.

Claim 6

A cell analyze apparatus according to Claim 1 further including: histogram generate means for generating a histogram of cell light information with respect to at least a parameter of the cell light information detected by said cell light information detect means; minimal point detect means for detecting a minimal point of the histogram created by said histogram generate means; and unnecessary information remove means for setting a noise threshold based on the minimal point detected by said minimal point detect means so as to remove, by use of the noise threshold, unnecessary information included in the cell light information detected by said cell light information detect means.

Claim 7

A cell analyze apparatus according to Claim 1 further including: sample supply means for sequentially sipping a plurality of samples to supply the sipped samples to said flow cell; and measurement condition set means for setting to said cell light information processing means an optimal measurement condition depending on the sample to be supplied by said sample supply means.

Claim 8

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to a plurality of parameterts; cell population subdivide means for detecting minimal points in histograms of cell light information obtained by said cell light information detect means with resepect to one or more paramters so as to subdivide a population of the cells in the cell float fluid based on the miniml points; cell light information collect means for collecting, based on one or more fractions of the fractions produced by said cell population subdivide means, cell light information of an objective cell population from the cell light information attained by said cell light information detect means; cell light information processing means for processing the cell light information of the objective cell population collected by said cell light information collect means; and output means for outputting results of the processing conducted by said cell light information processing means, said cell light information collect means having cell light information separate means, said cell light information separate means separating, from the cell light information detected by said cell light information detect means, cell light information of a cell population belonging to one or more fractions with respect to a paramter, said cell light information collect means collecting, from the separated cell light information, cell light information of a cell population belonging to a predetermined fraction with respect to other parameters.

Claim 9

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to a plurality of parameterts; cell population subdivide means for detecting minimal points in histograms of cell light information obtained by said cell light information detect means with resepect to one or more paramters so as to subdivide a population of the cells in the cell float fluid based on the miniml points; cell light information collect means for collecting, based on one or more fractions of the fractions produced by said cell population subdivide means, cell light information of an objective cell population from the cell light information attained by said cell light information detect means; cell light information processing means for processing the cell light information of the objective cell population collected by said cell light information collect means; and output means for outputting results of the processing conducted by said cell light information processing means, said cell light information collect means having cell light information separate means, said cell light information separate means separating, from the cell light information detected by said cell light information detect means, cell light information of a cell population belonging to one or more fractions with respect to a paramter, said cell light information collect means collecting, from the separated cell light information, cell light information of a cell population belonging to a predetermined fraction with respect to other parameters.

Claim 10

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to a plurality of parameterts; cell population subdivide means for subdividing, based on one or more paramters obtained by said cell light information detect means, a population of the cells in the cell float fluid; first cell light information collect means for collecting, based on one or more fractions of the fractions produced by said cell population subdivide means, cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information of the objective cell population collected by said cell light information collect means; output means for outputting results of the processing conducted by said cell light information processing means; maximum frequency point extract means for processing the cell light information of the objective cell population collected by said first cell light information collect means so as to extract a maximum frequency point on a cytogram of parameters associated with the fraction of the cell population; direct line generate means for radially drawing direct lines from the maximum frequency point extracted by said maximum frequency point extract means on the cytogram; boundary point extract means for extracting boundary points bsed on histograms on the respective direct lines produced by said direct line generate means; and second cell light information collect means for linking the boundary points extracted by said boundary point extract means to form a final fraction so as to collect cell light information of an objective cell population based on the final fraction.

Claim 11

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to a plurality of parameterts; cell population subdivide means for subdividing, based on one or more paramters obtained by said cell light information detect means, a population of the cells in the cell float fluid; first cell light information collect means for collecting, based on one or more fractions of the fractions produced by said cell population subdivide means, cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information of the objective cell population collected by said cell light information collect means; output means for outputting results of the processing conducted by said cell light information processing means; maximum frequency point extract means for processing the cell light information of the objective cell population collected by said first cell light information collect means so as to extract a maximum frequency point on a cytogram of parameters associated with the fraction of the cell population; direct line generate means for radially drawing direct lines from the maximum frequency point extracted by said maximum frequency point extract means on the cytogram; boundary point extract means for extracting boundary points bsed on histograms on the respective direct lines produced by said direct line generate means; and second cell light information collect means for linking the boundary points extracted by said boundary point extract means to form a final fraction so as to collect cell light information of an objective cell population based on the final fraction.

Claim 12

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to a plurallity of parameterts; cell population subdivide means for subdividing, based on one or more parameters obtained by said cell light information detect means, a population of the cells in the cell float fluid; first cell light information collect means for collecting, based on one or more fractions of the fractions produced by said cell population subdivide means, cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information of the objective cell population collected by said cell light information collect means; output means for outputting results of the processing conducted by said cell light information processing means; histogram generate means for processing the cell light information collected by said first cell light information collect means so as to attain, for each predetermined frequency of a parameter, a histogram of other parameters; boundary point extract means for extracting boundary points based on each histogram generated by said histogram generate means; and second cell light information collect means for linking the boundary points extracted by said boundary point extract means to form a final fraction so as to collect cell light information of an objective cell population based on the final fraction.

Claim 13

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to a plurallity of parameterts; cell population subdivide means for subdividing, based on one or more parameters obtained by said cell light information detect means, a population of the cells in the cell float fluid; first cell light information collect means for collecting, based on one or more fractions of the fractions produced by said cell population subdivide means, cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information of the objective cell population collected by said cell light information collect means; output means for outputting results of the processing conducted by said cell light information processing means; histogram generate means for processing the cell light information collected by said first cell light information collect means so as to attain, for each predetermined frequency of a parameter, a histogram of other parameters; boundary point extract means for extracting boundary points based on each histogram generated by said histogram generate means; and second cell light information collect means for linking the boundary points extracted by said boundary point extract means to form a final fraction so as to collect cell light information of an objective cell population based on the final fraction.

Claim 14

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to one or more parameterts; cell light information discriminate means for discriminating cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information discriminated by said cell light information discriminate means; output means for outputting results of the processing conducted by said cell light information processing means; histogram generate means for generating a histogram of cell light information with respect to at least a parameter of the cell light information detected by said cell light information detect means; minimal point detect means for detecting a minimal point of the histogram created by said histogram generate means; and unnecessary information remove means for setting a noise threshold based on the minimal point detected by said minimal point detect means so as to remove, by use of the noise threshold, unnecessary information included in the cell light information detected by said cell light information detect means.

Claim 15

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to one or more parameterts; cell light information discriminate means for discriminating cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information discriminated by said cell light information discriminate means; output means for outputting results of the processing conducted by said cell light information processing means; histogram generate means for generating a histogram of cell light information with respect to at least a parameter of the cell light information detected by said cell light information detect means; minimal point detect means for detecting a minimal point of the histogram created by said histogram generate means; and unnecessary information remove means for setting a noise threshold based on the minimal point detected by said minimal point detect means so as to remove, by use of the noise threshold, unnecessary information included in the cell light information detected by said cell light information detect means.

Claim 16

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to one or more parameterts; cell light information discriminate means for discriminating cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information discriminated by said cell light information discriminate means; output means for outputting results of the processing conducted by said cell light information processing means; sample supply means for sequentially sipping a plurality of samples to supply the sipped samples to said flow cell; and measurement condition set means for setting to said cell light information processing means an optimal measurement condition depending on the sample to be supplied by said sample supply means.

Claim 17

A cell analyze apparatus comprising: a flow cell through which a cell float fluid flows; a light source for irradiating a light beam onto cells flowing through said flow cell; cell light information detect means for detecting, for each said cell irradiated by the light beam, cell light information with respect to one or more parameterts; cell light information discriminate means for discriminating cell light information of an objective cell population from the cell light information detected by said cell light information detect means; cell light information processing means for processing the cell light information detected by said cell light information detect means and the cell light information discriminated by said cell light information discriminate means; output means for outputting results of the processing conducted by said cell light information processing means; sample supply means for sequentially sipping a plurality of samples to supply the sipped samples to said flow cell; and measurement condition set means for setting to said cell light information processing means an optimal measurement condition depending on the sample to be supplied by said sample supply means.