EP0359831B1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Biosensor and process for its production
Assignee: Panasonic Holdings Corp
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Filing: 1989-03-30
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Granted: 1995-08-30
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Expires: 2009-03-30
Abstract
Abstract A biosensor of the invention comprises an insulating base board (1) having formed thereon, in sequence, leads (2, 3, 3'), an electrode system mainly made of carbon (4, 5, 5'), an insulating layer (6) and a reaction layer (14) composed of an enzyme and an electron acceptor, and being provided thereon with a space (8) defined by a spacer (7) and a cover (9). When a biological sample solution is brought into contact with the inlet (10) of the biosensor having the above-described structure, the sample solution is introduced into its inside, while the air within the space (8) is rapidly discharged through the outlet (11) and, at the same time, the space (8) is filled with the sample solution up to the neighborhood of the outlet. Thus, measurement can be conducted inexpensively at a high speed with a high accuracy through simple procedures without residual bubbles.
Inventors
- 👤 Shiro Nankai
- 👤 Mariko Kawaguri
- 👤 Mayumi Ohtani
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 20:02
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; G01N27/3272; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
153 words
A biosensor for determining a substrate concentration in a sample solution comprising a base plate (1) comprising an electrode system (4,5;5′,41,42,43) being covered by a reaction layer (14) said electrode system (4,5;5′,41,42,43) and said reaction layer (14) having formed thereon a space (8;81,82) being defined by a spacer (7,7′) and a cover (9), said space (8,81,82) being provided with an introducing port (10) for introducing said sample solution into said space and a discharge port (11,12,13) for discharging the gas in said space (8,81,82) by inflow of said sample solution, said electrode system (4,5;5′,41,42,43) being equipped with at least an electrode for measurement (4,41,42,43) and a counter electrode (5,5′), at least an enzyme being carried on said reaction layer (14), a change in concentration of a substance in the reaction between said enzyme and said sample solution being detected with said electrode system (4,5;5′,41,42,43) to determine a substrate concentration in said sample solution.
Claim 2
Dependent Claim
38 words
A biosensor as claimed in claim 1, wherein said electrode system (5,41,42,43) comprises a plural set of electrode systems and reaction layers (14) corresponsing to said plural set of electrode system and a common space (8) are provided.
Claim 3
Dependent Claim
36 words
A biosensor as claimed in claim 1, wherein said electrode system (5;5′,41,42) comprises a plural set of electrode systems and, reaction layers (14) and spaces (81,82) corresponding to said plural set of electrode systems are provided.
Claim 4
Dependent Claim
73 words
A biosensor as claimed in claim 1, wherein an electrode system (4,5,5′;41,42,43) comprising at least an electrode for measurement (4;41,42,43) and a counter electrode (5,5′) is formed on an insulating base plate (1), a reaction layer (14) is formed on the surface of said electrode system (4,5,5′;41,42,43) and said reaction layer (14) comprises an enzyme layer composed of an oxidoreductase and a hydrophilic high molecular substance having formed thereon an electron acceptor layer.
Claim 5
Dependent Claim
78 words
A biosensor as claimed in claim 1, wherein an electrode system (4,5,5′;41,42,43) comprising at least an electrode for measurement (4,41;42,43) and a counter electrode (5,5′) is formed on an insulating base plate (1), a reaction layer (14) is formed on the surface of said electrode system (4,5,5′;41,42,43) and said reaction layer (14) comprises an enzyme layer composed of an oxidoreductase and a hydrophilic high molecular substance having formed thereon an electron acceptor layer containing a surface active agent.
Claim 6
Dependent Claim
31 words
A biosensor as claimed in claim 4 or 5, wherein said electron acceptor layer comprises fine particles of an electron acceptor having a particle size of not greater than 100 »m.
Claim 7
Dependent Claim
40 words
A biosensor as claimed in claim 4 or 5, wherein said hydrophilic high molecular substance is any member selected from hydrophilic high molecular substances of starch, carboxymethyl cellulose, gelatin, acrylate, vinyl alcohol, vinylpyrrolidone and maleic anhydride or a mixture thereof.
Claim 8
Dependent Claim
18 words
A biosensor as claimed in claim 1, wherein the surface of a material constructing said space is hydrophilic.
Claim 9
Dependent Claim
86 words
A biosensor as claimed in claim 2 or 3, wherein said electrode system (5,41,42) comprises two sets of electrode systems comprising at least an electrode for measurement (41,42) and a counter electrode (5,5′) mainly composed of carbon, a reaction layer (14) composed of a hydrophilic high molecular substance and an oxidoreductase being provided on one electrode system (5,5′,41) and a hydrophilic high molecular substance layer or a layer composed of a hydrophilic high molecular substance and an inactivated oxidoreductase being provided on another electrode system (5,5′,42).
Claim 10
Dependent Claim
81 words
A biosensor as claimed in claim 2 or 3, wherein said electrode (5,5′,41,42) system comprises two sets of electrode systems comprising at least an electrode for measurement (41,42) and a counter electrode (5,5′) mainly composed of carbon, a reaction layer composed of a hydrophilic high molecular substance, an oxidoreductase and an electron acceptor being provided on one electrode system (5,5′,41) and a layer composed of a hydrophilic high molecular substance and an electron acceptor being provided on another electrode system (5,5′,42).
Claim 11
Dependent Claim
38 words
A biosensor as claimed in claim 9 or 10, wherein said electrode system (5,5′;4;41,42,43) comprises an electrode for measurement (4;41,42,43) and a counter electrode (5,5′) mainly composed of carbon and a reference electrode (74) comprising a silver/silver chloride.
Claim 12
Dependent Claim
33 words
A biosensor as claimed in claim 1, wherein said electrode system (4,5,5′;41,42,43) is prepared from a material mainly composed of carbon formed on an insulating base (1) plate by means of screen printing.
Claim 13
Dependent Claim
79 words
A biosensor as claimed in claim 1, wherein an electrode system (4,5,5′;41,42,43) comprising at least an electrode for measurement (4;41,42,43) and a counter electrode (5,5′) is formed on an insulating base plate (1), a reaction layer (14) is formed on said electrode system and said reaction layer comprises a first layer composed of a hydrophilic high molecular substance and an oxidoreductase, a second layer composed of a hydrophilic high molecular substance and a third layer containing an electron acceptor.
Claim 14
Dependent Claim
44 words
A biosensor as claimed in claim 13, wherein said hydrophilic high molecular substance in the first layer and the second layer is selected from hydrophilic high molecular substances of starch, carboxymethyl cellulose, gelatin, acrylate, vinyl alcohol, vinylpyrrolidone and maleic anhydride or a mixture thereof.
Claim 15
Dependent Claim
20 words
A biosensor as claimed in any of claims 1 to 14, wherein said spacer and said cover are formed integrally.
Claim 16
Dependent Claim
103 words
A process for preparing a biosensor which comprises forming an electrode system comprising at least an electrode for measurement and a counter electrode on an insulating base plate, coating a hydrophilic high molecular substance aqueous solution and an oxidoreductase aqueous solution on said electrode system and then drying to form an enzyme layer, spreading a mixture of an electron acceptor and an organic solvent onto said enzyme layer, removing said organic solvent to form an electron acceptor layer and then integrating this assembly with a cover for forming a space for receiving a sample and having an introducing port and a discharge port.
Claim 17
Dependent Claim
46 words
A process for preparing a biosensor according to claim 16, wherein prior to the formation of said electron acceptor layer a solution of a hydrophilic high molecular substance in an organic solvent is spreaded onto said enzyme layer to form a hydrophilic high molecular substance layer.
Claim 18
Dependent Claim
46 words
A process for preparing a biosensor as claimed in claim 16 or 17, wherein a mixture of said electron acceptor, said surface active agent and said organic solvent is spread on said enzyme layer and said organic solvent is removed to form an electron acceptor layer.
Claim 19
Dependent Claim
33 words
A process for preparing a biosensor as claimed in claim 18, wherein said electron acceptor layer comprises fine particles of an electron acceptor having a particle size of not greater than 100 »m.
References Cited (Backward Citations)
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