EP0422209B1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Implants for large volumes of cells on polymeric matrices
Assignee: Boston Children's Hospital
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Filing: 1990-04-25
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Granted: 1995-03-15
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Expires: 2010-04-25
Abstract
Abstract The present invention is a method and means whereby large volumes of cells having a desired function are attached to, and, optionally, proliferated, on a biocompatible degradable, non-degradable or combination degradable-non-degradable scaffolding and transferred with mininal trauma and blood loss into a patient at a site appropriate for attachment, growth and function to produce a functional organ equivalent in the absence of vascularization of the implanted cell mass. In the preferred embodiment, multiple cell-matrix structures are implanted between folds of the mesentery. The method is particularly well suited for growth of endocrine structures, including liver, pancreas, and adrenal gland, as well as other tissues. The preferred material for forming the matrix or support structure is a biodegradable artificial polymer. Materials such as angiogenesis factors can be incorporated into degradable matrices for use in preparing the implantation sites prior to, or at the time of, implanting the cells. The function and viability of the attached cells can also be manipulated by treatment or coatiing of the matrix structure to increase the number of attachment sites.
Inventors
- 👤 Joseph P. Vacanti
- 👤 Robert S. Langer
- 👤 Lynt Johnson
- 👤 Linda Griffith-Cima
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 22:05
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61F; A61F2/00; A61F2/02; A61F2/022; A61L; A61L27/00; A61L27/36; A61L27/38; C12N5/06; C12N5/0602; C12N5/067; C12N5/0671; C12N2501/00; C12N2501/10; C12N2501/18; C12N2533/00; C12N2533/30; C12N2533/40
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
68 words
The use of a matrix structure comprising a biocompatible material in a fibrous shape having interstitial spacing in the range of 100 to 200 »m and having viable parenchymal cells attached thereto in the manufacture of a surgical implant for use in a method of surgery comprising implanting a plurality of matrices between folds of tissue having high surface area and vasculature adjacent the surface of the tissue.
Claim 2
Dependent Claim
17 words
The use according to Claim 1 wherein the tissue is the mesentery, the omentum or the peritoneum.
Claim 3
Dependent Claim
34 words
The use according to Claim 1 or 2 wherein the material is a biodegradable polymer selected from the group consisting of polylactic acid, polyglycolic acid, polyorthoester, polyanhydride, collagen, and copolymers, blends and combinations thereof.
Claim 4
Dependent Claim
39 words
The use according to Claim 1 or 2 wherein the material is a non-degradable, or non-absorbable, material selected from the group consisting of polypropylene, polyethylene terephthalate and other polyesters, polytetrafluoroethylene, ethylene vinyl acetate, nylon, stainless steel, and combinations thereof.
Claim 5
Dependent Claim
46 words
The use according to any one of Claims 1 to 4 wherein the matrix material is coated with an attachment factor selected from the group consisting of basement membrane components, agar, agarose, gelatin, gum arabic, collagens, fibronectin, laminin, hyaluronic acid, glycosaminoglycans, attachment peptides, and mixtures thereof.
Claim 6
Dependent Claim
61 words
The use according to any one of Claims 1 to 5 wherein the matrix further comprises a compound selected from the group consisting of angiogenic compounds, nutrients, growth factors, inducers of differentiation or de-differentiation, products of secretion, immunomodulators, inhibitors of inflammation, regression factors, biologically active compounds which enhance or allow ingrowth of the lymphatic network or nerve fibres, and mixtures thereof.
Claim 7
Dependent Claim
46 words
The use according to any one of Claims 1 to 6 wherein the cells are selected from the group consisting of hepatocytes, pancreatic cells, adrenal cells, lymphoid cells, cells of the nervous system, fibroblasts, endothelial cells, lymphatic cells, splenic cells, and cells of the genitourinary system.
Claim 8
Dependent Claim
20 words
The use according to any one of Claims 1 to 7 wherein the matrix structure has a fibrous mesh-like shape.
Claim 9
Dependent Claim
19 words
The use according to any one of Claims 1 to 7 wherein the matrix structure has a sponge-like shape.
Claim 10
Dependent Claim
18 words
The use according to any one of Claims 1 to 7 wherein the matrix structure is a sheet.
Claim 11
Dependent Claim
21 words
The use according to Claim 10 wherein the thickness of the sheet is in the range 200 »m to 2 mm.
Claim 12
Dependent Claim
51 words
The use according to any one of Claims 1 to 3 or any of Claims 5 to 11 when not dependent on Claim 4 formed by coating a biocompatible fibrous mesh with a second biocompatible, biodegradable polymer solution, wherein the fibrous mesh material is not soluble in the second polymer solution.
Claim 13
Dependent Claim
32 words
The use according to Claim 12 wherein the fibrous mesh is formed of poly(glycolic acid) and the second polymer is selected from the group of poly(lactic acid) and poly(lactic acid-glycolic acid) copolymers.
Claim 14
Dependent Claim
22 words
The use according to Claim 12 or 13 wherein the second polymer forms flat surfaces between the fibers forming the fibrous mesh.
Claim 15
Dependent Claim
47 words
A matrix sheet structure comprising a biocompatible material in a fibrous shape having interstitial spacing in the range of 100 to 200 »m and having viable parenchymal cells attached thereto wherein the thickness of the matrix structure is in the range of 200 »m to 2 mm.
Claim 16
Dependent Claim
24 words
A matrix sheet structure according to Claim 15 wherein the thickness of the matrix structure is in the range of 200 to 300 »m.
Claim 17
Dependent Claim
34 words
A matrix sheet structure according to Claim 15 wherein the material is a biodegradable polymer selected from the group consisting of polylactic acid, polyglycolic acid, polyorthoester, polyanhydride, collagen, and copolymers, blends and combinations thereof.
Claim 18
Dependent Claim
39 words
A matrix sheet structure according to Claim 15 wherein the material is a non-degradable, or non-absorbable material selected from the group consisting of polypropylene, polyethylene terephthalate and other polyesters, polytetrafluoroethylene, ethylene vinyl acetate, nylon, stainless steel, and combinations thereof.
Claim 19
Dependent Claim
48 words
A matrix sheet structure according to any one of Claims 15 to 18 wherein the matrix material is coated with an attachment factor selected from the group consisting of basement membrane components, agar, agarose, gelatin, gum arabic, collagens, fibronectin, laminin, hyaluronic acid, glycosaminoglycans, attachment peptides, and mixtures thereof.
Claim 20
Dependent Claim
63 words
A matrix sheet structure according to any one of Claims 15 to 19 wherein the matrix further comprises a compound selected from the group consisting of angiogenic compounds, nutrients, growth factors, inducers of differentiation or de-differentiation, products of secretion, immunomodulators, inhibitors of inflammation, regression factors, biologically active compounds which enhance or allow ingrowth of the lymphatic network or nerve fibres, and mixtures thereof.
Claim 21
Dependent Claim
46 words
A matrix sheet structure according to any one of Claims 15 to 20 wherein the cells are selected from the group consisting of hepatocytes, pancreatic cells, adrenal cells, lymphoid cells, cells of the nervous system, fibroblasts, endothelial cells, splenic cells, and cells of the genitourinary system.
Claim 22
Dependent Claim
53 words
A matrix sheet structure according to any one of Claims 15 to 17 or any of Claims 19 to 21 when not dependent on Claim 18 formed by coating a biocompatible fibrous mesh with a second biocompatible, biodegradable polymer solution, wherein the fibrous mesh material is not soluble in the second polymer solution.
Claim 23
Dependent Claim
34 words
A matrix sheet structure according to Claim 22 wherein the fibrous mesh is formed of poly(glycolic acid) and the second polymer is selected from the group of poly(lactic acid) and poly(lactic acid-glycolic acid) copolymers.
Claim 24
Dependent Claim
24 words
A matrix sheet structure according to Claim 22 or 23 wherein the second polymer forms flat surfaces between the fibers forming the fibrous mesh.
References Cited (Backward Citations)
No citation records indexed.
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