EP0424871A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Leukocytes and hemiglobin in blood
Assignee: Sysmex Corp
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Filing: 1990-10-23
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Granted: —
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Expires: 2010-10-23
Abstract
Abstract A reagent for measuring the hemoglobin concentration and counting the number of leukocytes in a blood sample, which contains at least one of a quaternary ammonium salt or a pyridinium salt, having a concentration capable of hemolyzing erythrocytes in the blood and denaturing hemoglobin and at least one of cationic, nonionic or amphoteric surfactants or an oxidant capable of oxidizing heme in hemoglobin, said reagent being capable of dividing leukocytes into two or three fractions, for example, a fraction of lymphocytes, a fraction of monocytes, eosinophils and basophils, and a fraction of neutrophils.
Inventors
- 👤 Syouzou Toda
- 👤 Takashi Sakata
- 👤 Yukio Hamaguchi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 08:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/5005; G01N33/5094; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
367 words
A reagent for enabling measuring a hemoglobin concentration to be measured and counting the number of leukocytes present in a blood sample to be counted, which is free of cyanides and satisfies the following conditions: i) it contains at least one of a quaternary ammonium salt (formula a) or a pyridinium salt (formula b), having a concentration capable of hemolyzing erythrocytes in the blood and denaturing hemoglobin: where R₁: C₈ to C₂₀ alkyl, alkenyl or alkinyl group, R₂, R₃ and R₄: C₁ to C₈ alkyl, alkenyl or alkinyl group, and X⁻: anionic group; where n: an integer of from 7 to 19, and X⁻: an anionic group; ii) it also contains at least one compound selected from the group consisting of cationic, nonionic or amphoteric surfactants as shown by the formulas c to e and an oxidant capable of oxidizing heme in hemoglobin as shown by the formula f: where R₁: C₈ to C₂₀ alkyl, alkenyl or alkinyl group, R₂ and R₃: C₁ to C₈ alkyl, alkenyl or alkinyl group, and X⁻: anionic group; formula d R₁ - R₂ - (CH₂CH₂O) n - H where R₁: C₈ to C₂₀ alkyl, alkenyl or alkinyl group, R₂: O, or COO, and n: an integer of from 10 to 50; where R₁: C₈ to C₂₀ alkyl group, and R₂ and R₃: C₁ to C₈ alkyl, alkenyl or alkinyl group; f.....nitrite ion, quinone compound, alloxan, methylene blue, aniline, acetanilide, nitrobenzene, acetophenetidine, nitrotoluene, sulfonamide, phenylhydrazine, ascorbic acid or aminophenol; iii) it is a solution with a pH ranging from 3.0 to 9.0; iv) the total concentration of the quaternary ammonium salt is in the range of from 0.1 to 15.0 g/ℓ; v) the total concentration of the pyridinium salt is in the range of from 0.1 to 15. 0 g/ℓ; vi) the total concentration of the oxidant is in the range of from 0.5 to 10.0 g/ℓ; vii) the total concentration of the nonionic surfactant is in the range of from 0.1 to 15.0 g/ℓ; viii) the total concentration of the amphoteric surfactant is in the range of from 0.1 to 15.0 g/ℓ; and ix) the total concentration of the cationic surfactant is in the range of from 0.1 to 15.0 g/ℓ.
Claim 2
Dependent Claim
367 words
A reagent for enabling measuring a hemoglobin concentration to be measured and counting the number of leukocytes present in a blood sample to be counted, which is free of cyanides and satisfies the following conditions: i) it contains at least one of a quaternary ammonium salt (formula a) or a pyridinium salt (formula b), having a concentration capable of hemolyzing erythrocytes in the blood and denaturing hemoglobin: where R₁: C₈ to C₂₀ alkyl, alkenyl or alkinyl group, R₂, R₃ and R₄: C₁ to C₈ alkyl, alkenyl or alkinyl group, and X⁻: anionic group; where n: an integer of from 7 to 19, and X⁻: an anionic group; ii) it also contains at least one compound selected from the group consisting of cationic, nonionic or amphoteric surfactants as shown by the formulas c to e and an oxidant capable of oxidizing heme in hemoglobin as shown by the formula f: where R₁: C₈ to C₂₀ alkyl, alkenyl or alkinyl group, R₂ and R₃: C₁ to C₈ alkyl, alkenyl or alkinyl group, and X⁻: anionic group; formula d R₁ - R₂ - (CH₂CH₂O) n - H where R₁: C₈ to C₂₀ alkyl, alkenyl or alkinyl group, R₂: O, or COO, and n: an integer of from 10 to 50; where R₁: C₈ to C₂₀ alkyl group, and R₂ and R₃: C₁ to C₈ alkyl, alkenyl or alkinyl group; f.....nitrite ion, quinone compound, alloxan, methylene blue, aniline, acetanilide, nitrobenzene, acetophenetidine, nitrotoluene, sulfonamide, phenylhydrazine, ascorbic acid or aminophenol; iii) it is a solution with a pH ranging from 3.0 to 9.0; iv) the total concentration of the quaternary ammonium salt is in the range of from 0.1 to 15.0 g/ℓ; v) the total concentration of the pyridinium salt is in the range of from 0.1 to 15. 0 g/ℓ; vi) the total concentration of the oxidant is in the range of from 0.5 to 10.0 g/ℓ; vii) the total concentration of the nonionic surfactant is in the range of from 0.1 to 15.0 g/ℓ; viii) the total concentration of the amphoteric surfactant is in the range of from 0.1 to 15.0 g/ℓ; and ix) the total concentration of the cationic surfactant is in the range of from 0.1 to 15.0 g/ℓ.
Claim 3
Dependent Claim
54 words
The reagent according to Claim 1 containing at least two of said quaternary ammonium salts, with the total concentration of said quaternary ammonium salt or salts ranging between 0.1 g/ℓ and 12.0 g/ℓ, and at least one of oxidants selected from f, said reagent being capable of dividing leukocytes into at least two fractions.
Claim 4
Dependent Claim
103 words
The reagent according to Claim 1 containing at least one of said quaternary ammonium salts, with the total concentration of said quaternary ammonium salt or salts ranging between 0.1 g/ℓ and 12.0 g/ℓ, said reagent also containing at least one of said nonionic surfactants, with the total concentration of said nonionic surfactant or surfactants ranging between 0.1 g/ℓ and 12.0 g/ℓ, said reagent also containing at least one of oxidants selected from f, with the total concentration of said oxidant or oxidants ranging between 0.5 g/ℓ and 10.0 g/ℓ, and said reagent being capable of dividing the leukocytes into at least two fractions.
Claim 5
Dependent Claim
75 words
The reagent according to Claim 1 containing at least one of said quaternary ammonium salts, with the total concentration of said quaternary ammonium salt or salts ranging between 0.1 g/ℓ and 12.0 g/ℓ, said reagent also containing at least one of said amphoteric surfactants, with the total concentration of said amphoteric surfactant or surfactants ranging between 0.1 g/ℓ and 12.0 g/ℓ, and said reagent being capable of dividing the leukocytes into at least two fractions.
Claim 6
Dependent Claim
75 words
The reagent according to Claim 1 containing at least one of said quaternary ammonium salts, with the total concentration of said quaternary ammonium salt or salts ranging between 1.0 g/ℓ and 10.0 g/ℓ, said reagent also containing at least one of said nonionic surfactants, with the total concentration of said nonionic surfactant or surfactants ranging between 0.1 g/ℓ and 12.0 g/ℓ, and said reagent being capable of dividing the leukocytes into at least two fractions.
Claim 7
Dependent Claim
75 words
The reagent according to Claim 1 containing at least one of said quaternary ammonium salts, with the total concentration of said quaternary ammonium salt or salts ranging between 1.0 g/ℓ and 12.0 g/ℓ, said reagent also containing at least one of said cationic surfactants, with the total concentration of said cationic surfactant or surfactants ranging between 0.1 g/ℓ and 5.0 g/ℓ, and said reagent being capable of dividing the leukocytes into at least two fractions.
Claim 8
Dependent Claim
73 words
The reagent according to Claim 1 containing at least two of said quaternary ammonium salts, with the total concentration of said quaternary ammonium salt of salts ranging between 2.2 g/ℓ and 5.0 g/ℓ, said reagent also containing at least one of oxidants selected from f, with the total concentration of said oxidant or oxidants ranging between 0.5 g/ℓ and 10.0 g/ℓ, and said reagent being capable of dividing the leukocytes into three fractions.
Claim 9
Dependent Claim
73 words
The reagent according to Claim 1 containing at least one of said quaternary ammonium salts, with the total concentration of said quaternary ammonium salt or salts ranging between 1.0 g/ℓ and 10.0 g/ℓ, said reagent also containing at least one of said pyridinium salts, with the total concentration of said pyridinium salt or salts ranging between 1.0 g/ℓ and 5.0 g/ℓ, and said reagent being capable of dividing the leukocytes into three fractions.
Claim 10
Dependent Claim
73 words
The reagent according to Claim 1 containing at least one of said quaternary ammonium salts, with the total concentration of the quaternary ammonium salt or salts ranging between 3.0 g/ℓ and 12.0 g/ℓ, said reagent also containing at least one of said nonionic surfactants, with the total concentration of said nonionic surfactant or surfactants ranging between 1.0 g/ℓ and 12.0 g/ℓ, and said reagent being capable of dividing the leukocytes into three fractions.
Claim 11
Dependent Claim
73 words
The reagent according to Claim 1 containing at least one of said quaternary ammonium salts, with the total concentration of the quaternary ammonium salt or salts ranging between 1.0 g/ℓ and 10.0 g/ℓ, said reagent also containing at least one of said amphoteric surfactants, with the total concentration of said amphoteric surfactant or surfactants ranging between 0.1 g/ℓ and 10.0 g/ℓ, and said reagent being capable of dividing the leukocytes into three fractions.
References Cited (Backward Citations)
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