EP0451848A2
Expired / Lapsed
EP
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Immunoassay element and process for immunoassay
Assignee: Fujirebio Inc
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Filing: 1991-04-11
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Granted: —
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Expires: 2011-04-11
Abstract
Abstract An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.
Inventors
- 👤 Hiraoka C/O Fuji Photo Film Co. Ltd. Toshikage
- 👤 Tetsuji C/O Fujirebio Inc. Tanimoto
- 👤 Yoshihiko C/O Fuji Photo Film Co. Ltd. Makino
- 👤 Tadashi C/O Fujirebio Inc. Ninomiya
- 👤 Naofumi C/O Fuji Photo Film Co. Ltd. Hora
- 👤 Yoshihiro C/O Fujirebio Inc. Ashihara
- 👤 Yukio C/O Fuji Photo Film Co. Ltd. Sudo
- 👤 Toshihiro C/O Fuji Photo Film Co. Ltd. Mori
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 06:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54391; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S435/969; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
79 words
An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between said ligand, a linked product of said ligand and a high molecular weight compound and an enzyme-labelled antibody, said element comprising a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of said enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting said difussible material into a lower molecular weight product.
Claim 2
Dependent Claim
25 words
The immunoassay element according to claim 1, wherein said enzyme-labelled antibody is contained in said substrate layer or a layer superposed on said substrate layer.
Claim 3
Dependent Claim
34 words
The immunoassay element according to claim 1, wherein said linked product of said ligand and said high molecular weight compound is contained in said substrate layer or a layer superposed on said substrate layer.
Claim 4
Dependent Claim
30 words
The immunoassay element according to claim 1, wherein said enzyme-labelled antibody and said linked product of said ligand and said high molecular weight compound are contained in said substrate layer.
Claim 5
Dependent Claim
27 words
The immunoassay element according to claim 1, wherein the labelling enzyme of said enzyme-labelled antibody is an endo-active glucosidase and said fragmenting enzyme is an exo-active glucosidase.
Claim 6
Dependent Claim
15 words
The immunoassay element according to claim 5, wherein said lower molecular weight product is glucose.
Claim 7
Dependent Claim
38 words
The immunoassay element according to claim 5, wherein said reagent layer or another water-permeable layer contains a reagent composition which reacts with said fragmented product to form a dye having an absorption peak in the visible wavelength range.
Claim 8
Dependent Claim
20 words
The immunoassay element according to claim 7, wherein said reagent composition reacts with said fragmented product to form a peroxide.
Claim 9
Dependent Claim
19 words
The immunoassay element according to claim 8, wherein said reagent composition contains a leuco dye which colors upon oxidation.
Claim 10
Dependent Claim
28 words
The immunoassay element according to claim 9, wherein said reagent composition contains a dispersion in an aqueous liquid of a solution of leuco dye in a water-insoluble solvent.
Claim 11
Dependent Claim
19 words
The immunoassay element according to claim 10, wherein said reagent composition contains glucose oxidase, peroxidase and a leuco dye.
Claim 12
Dependent Claim
116 words
An immunoassaying process for quantitatively analyzing an amount of a ligand in a sample by determining the change in enzymatic activity caused by a reaction between said ligand, a linked product of said ligand and a high molecular weight compound and an enzyme-labelled antibody, comprising the steps of: (a) applying said sample on a substrate layer containing a non-diffusible substrate for forming a diffusible material in the presence of said enzyme; (b) allowing to migrate said diffusible material formed in said substrate layer into a reagent layer containing a fragmenting enzyme for further decomposing to a lower molecular weight product; and (c) measuring the amount of said lower molecular weight product formed in said reagent layer.
References Cited (Backward Citations)
No citation records indexed.
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