EP0457430A2
Expired / Lapsed
EP
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Collagen constructs
Assignee: Organogenesis Inc
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Filing: 1991-04-05
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Granted: —
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Expires: 2011-04-05
Abstract
Abstract The present invention provides collagen constructs and methods of making and using such constructs. The present invention also provides tissue equivalents having improved characteristics and methods of making and using such tissue equivalents. This invention also provides methods of producing highly concentrated solutions of collagen.
Inventors
- 👤 Paul David Kemp
- 👤 Robert Michael Carr
- 👤 John Gregory Maresh
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 15:10
CPC Classifications
D; D01; D01F; D01F4/00; A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; A61L27/36; A61L27/38; A61L27/3804; A61L27/3808; A61L27/3813; A61L27/50; A61L27/507; A61L27/60; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; Y; Y10; Y10S; Y10S623/00; Y10S623/901
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
59 words
A method of forming a construct comprising collagen, the method comprising the steps of: (a) providing a solution comprising collagen adjacent a permeable member, the permeable member being in contact with a concentrating agent; and (b) maintaining the collagen solution, permeable member and concentrating agent under conditions sufficient to enable the collagen construct to form at the permeable member.
Claim 2
Dependent Claim
18 words
The method of claim 1, further comprising the step of causing fibrils to form in the collagen construct.
Claim 3
Independent Claim
22 words
The method of claims 1 or 2, wherein the concentrating agent has an osmotic pressure higher than that of the collagen solution.
Claim 4
Independent Claim
14 words
The method of claims 1 or 2, wherein the concentrating agent comprises a polymer.
Claim 5
Dependent Claim
20 words
The method of claim 4, wherein the concentrating agent comprises at least one of polyethylene glycol and DEXTRAN R .
Claim 6
Dependent Claim
16 words
The method of claim 4, wherein the concentrating agent comprises a polymer in a salt solution.
Claim 7
Dependent Claim
21 words
The method of claim 6, wherein the concentrating agent comprises a solution of polyethylene glycol or DEXTRAN R in sodium chloride.
Claim 8
Dependent Claim
28 words
The method of claim 7, wherein the concentrating agent comprises a solution of 20% weight/volume polyethylene glycol having a molecular weight of about 8000 in phosphate buffered saline.
Claim 9
Dependent Claim
36 words
The method of claim 2, wherein the concentrating agent comprises a polymer in a salt solution, and the fibrils are caused to form by means of increasing the pH and ionic strength of the concentrating agent.
Claim 10
Dependent Claim
37 words
The method of claim 2, wherein the fibrils are caused to form in the collagen construct by increasing at least two of (a) the pH, (b) the salt concentration, or (c) the temperature of the collagen construct.
Claim 11
Independent Claim
20 words
The method of claims 1 or 2, wherein the collagen concentration of the construct is about 50 to 100 mg/ml.
Claim 12
Independent Claim
20 words
The method of claims 1 or 2, wherein the collagen concentration of the construct is at least about 100 mg/ml.
Claim 13
Independent Claim
14 words
The method of claims 1 or 2, wherein the permeable member comprises a membrane.
Claim 14
Dependent Claim
22 words
The method of claim 13, wherein the membrane comprises a porous ceramic or stainless steel material, dialysis tubing, or a nuclepore membrane.
Claim 15
Independent Claim
15 words
The method of claims 1 or 2, wherein the permeable member is flat or tubular.
Claim 16
Independent Claim
18 words
The method of claims 1 or 2, further comprising the step of providing cells to the collagen construct.
Claim 17
Dependent Claim
17 words
The method of claim 16, wherein the cells are at least one of endothelial and epithelial cells.
Claim 18
Independent Claim
18 words
The method of claims 1 or 2, further comprising the step of forming pores in the collagen construct.
Claim 19
Dependent Claim
14 words
The method of claim 18, wherein pores are formed by lyophilyzing the collagen construct.
Claim 20
Dependent Claim
35 words
The method of claim 18, wherein the pores are formed by means of incorporating a polymer in the collagen construct and the method further comprises the step of removing the polymer from the collagen construct.
Claim 21
Dependent Claim
14 words
The method of claim 20, wherein the polymer is polyvinyl alcohol or hyaluronic acid.
Claim 22
Dependent Claim
17 words
The method of claim 2, further comprising the step of cross-linking the fibrils in the collagen construct.
Claim 23
Dependent Claim
18 words
The method of claim 22, wherein the fibrils are cross-linked by air-drying, lyophilizing or contact with an aldehyde.
Claim 24
Dependent Claim
20 words
The method of claim 22, wherein the collagen construct has a burst strength of about 300 to 1000 mm Hg.
Claim 25
Dependent Claim
19 words
The method of claim 22, wherein the collagen construct has a burst strength of greater than 1000 mm Hg.
Claim 26
Independent Claim
30 words
The method of claims 1 or 2, wherein the collagen solution is at a concentration of about 5 to 10 mg/ml collagen and a pH of about 2 to 4.
Claim 27
Independent Claim
17 words
The method of claims 1 or 2, wherein the collagen construct is provided with a support member.
Claim 28
Dependent Claim
12 words
The method of claim 27, wherein the support member comprises a polyester.
Claim 29
Independent Claim
30 words
The method of claims 1 or 2, wherein the collagen solution further comprises at least one of an angiogenic factor, an anti-inflammatory agent, a chemotactic agent, or a collagenase inhibitor.
Claim 30
Independent Claim
88 words
A method of forming a tubular collagen construct in accordance with claims 1 or 2 by use of a device having an inner and outer chamber, the inter,face between the inner and outer chamber comprising a tubular permeable member, the method comprising the steps of: (a) providing the solution comprising collagen the outer chamber; (b) providing the concentrating agent to the inner chamber; and (c) maintaining the collagen solution, permeable member and concentrating agent under conditions sufficient to enable the coloagen construct to form at the permeable member.
Claim 31
Independent Claim
88 words
A method of forming a tubular collagen construct in accordance with claims 1 or 2 by use of device having an inner and outer chamber, e interface between the inner and outer chamber comprising a tubular permeable member, the method comprising the steps of: (a) providing the solution comprisong collagen to the inner chamber; (b) providing the concentrating agent to the outer chamber; and (c) maintaining the collagen solution, permeable member and concentrating agent under conditions sufficient to enable the collagen construct to form at the permeable member.
Claim 32
Independent Claim
149 words
A method of forming a multi-layer tubular collagen construct by use of a device having an inner and outer chamber, the interface between the inner and outer chamber comprising a tubular permeable member, the method comprising the steps of: (a) providing a first solution comprising collagen at a pH of about 2 to 4 to the outer chamber; (b) providing a concentrating agent to the inner chamber; (c) maintaining the first collagen solution, permeable member and concentrating agent under conditions sufficient to enable the tubular collagen construct to form at the permeable member; (d) replacing the first collagen solution with a second solution comprising collagen; and (e) maintaining the second collagen solution, permeable member and concentrating agent under conditions sufficient to enable a second tubular construct to form outwardly of the first tubular construct;and (f) repeating steps (d) and (e) if additional layers of tubular collagen construct are desired.
Claim 33
Dependent Claim
23 words
The method of claim 32, further comprising the step of causing fibrils to form in at least one layer of the collagen construct.
Claim 34
Dependent Claim
25 words
A tubular construct in accordance with claim 33, wherein the construct is provided with living cells to form a blood vessel or skin tissue equivalent.
Claim 35
Independent Claim
82 words
A method of forming a flat collagen construct by use of a device having an inner and outer container, the interface between the inner and outer chamber comprising a flat permeable member, the method comprising the steps of: (a) providing a solution comprising collagen to the inner chamber; (b) providing a concentrating agent to the outer chamber; and (c) maintaining the collagen solution, permeable member and concentrating agent under conditions sufficient to enable the flat construct to form at the permeable member.
Claim 36
Dependent Claim
25 words
A flat construct in accordance with claim 35, wherein the construct is provided with living cells to form a blood vessel or skin tissue equivalent.
Claim 37
Dependent Claim
11 words
A collagen construct formed in accordance with claims 1 or 2.
Claim 38
Dependent Claim
20 words
A tissue equivalent comprising a construct formed in accordance with claims 1 or 2 and one or more cell types.
Claim 39
Independent Claim
58 words
A method of concentrating a solution of collagen comprising: (a) contacting a collagen solution with a permeable member, the permeable member being in contact with an agent for concentrating the collagen; (b) maintaining the collagen solution and concentrating agent under conditions sufficient to enable the collagen solution to reach a concentration of from about 50 to 100 mg/ml.
Claim 40
Dependent Claim
16 words
The method of claim 39, wherein the collagen solution reaches a concentration of about 100 mg/ml.
References Cited (Backward Citations)
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