EP0506477A1
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Vascular endothelial cell growth factor C subunit
Assignee: Merck Sharp & Dohme LLC
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Filing: 1992-03-27
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Granted: —
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Expires: 2012-03-27
Abstract
Abstract Vascular endothelial cell growth factor C subunit DNA is prepared by polymerase chain reaction techniques. The DNA encodes a protein that may exist as either a heterodimer or homodimer. The protein is a mammalian vascular endothelial cell mitogen and as such is useful for the promotion of vascular development and repair. This unique growth factor is also useful in the promotion of tissue repair.
Inventors
- 👤 Marvin L. Bayne
- 👤 Kenneth A. Thomas, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 07:53
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6897; A; A61; A61P; A61P43/00; A61P9/00; A61P9/14; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C12N; C12N15/00; C12N15/09; C12N15/10; C12Q1/6876
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
16 words
A purified and isolated DNA sequence encoding the C subunit of vascular endothelial cell growth factor.
Claim 2
Dependent Claim
14 words
A purified and isolated vascular endothelial cell growth factor C subunit DNA sequence comprising:
Claim 3
Dependent Claim
20 words
Vascular endothelial cell growth growth factor AC DNA comprising an A subunit DNA sequence and a C subunit DNA sequence.
Claim 4
Dependent Claim
20 words
Vascular endothelial cell growth growth factor BC DNA comprising a B subunit DNA sequence and a C subunit DNA sequence.
Claim 5
Dependent Claim
14 words
A purified and isolated vascular endothelial cell growth factor C subunit DNA sequence comprising:
Claim 6
Dependent Claim
60 words
Vascular endothelial cell growth factor AC DNA comprising an A subunit DNA sequence selected form the group consisting of: a DNA sequence encoding an 189 amino acid form, a DNA sequence encoding an 165 amino acid form and a DNA sequence encoding a 121 amino acid form, with said A subunit DNA operably attached to a C subunit DNA sequence.
Claim 7
Dependent Claim
52 words
Vascular endothelial cell growth factor BC DNA comprising a B subunit DNA sequence selected form the group consisting of a DNA sequence encoding a 135 amino acid form; and a DNA sequence encoding a 115 amino acid form, with said B subunit DNA sequence operably attached to a C subunit DNA sequence.
Claim 8
Dependent Claim
11 words
Homodimeric vascular endothelial growth factor DNA comprising C subunit DNA sequences.
Claim 9
Dependent Claim
14 words
A vector containing the DNA sequence of any one of claims 3 to 8.
Claim 10
Dependent Claim
20 words
A host cell transformed by the vector of claim 9 containing the DNA sequence encoding vascular endothelial cell growth factor.
Claim 11
Dependent Claim
39 words
A process for the preparation of vascular endothelial cell growth factor comprising culturing the transformed host cell of claim 10 under conditions suitable for the expression of vascular endothelial cell growth factor and recovering vascular endothelial cell growth factor.
Claim 12
Dependent Claim
11 words
Vascular endothelial growth factor made by the process of claim 11.
Claim 13
Dependent Claim
20 words
Vascular endothelial cell growth factor AC comprising an A subunit amino acid sequence and a C subunit amino acid sequence.
Claim 14
Dependent Claim
20 words
Vascular endothelial cell growth factor BC comprising a B subunit amino acid sequence and a C subunit amino acid sequence.
Claim 15
Dependent Claim
20 words
Vascular endothelial cell growth factor CC comprising a C subunit amino acid sequence and a C subunit amino acid sequence.
Claim 16
Dependent Claim
15 words
A purified and isolated vascular endothelial cell growth factor C subunit amino acid sequence comprising:
Claim 17
Dependent Claim
15 words
A purified and isolated vascular endothelial cell growth factor C subunit amino acid sequence comprising:
Claim 18
Dependent Claim
30 words
A tissue repairing pharmaceutical composition comprising a pharmaceutical carrier and an effective tissue repairing amount of the purified vascular endothelial growth factor of any one of claims 13 to 15,
Claim 19
Dependent Claim
27 words
The use of the vascular endothelial cell growth factor of any one of claims 13 to 15 for the manufacture of a medicament for promoting tissue repair.
Claim 20
Dependent Claim
29 words
The use of the vascular endothelial cell growth factor of any one of claims 13 to 15 for the manufacture of a medicament for stimulating vascular endothelial cell growth.
References Cited (Backward Citations)
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