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EP0720012A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Flow cytometer

Assignee: Sysmex Corp
•
Filing: 1995-12-21
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Granted: —
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Expires: 2015-12-21
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Abstract

Abstract A flowcytometer includes a sheath flow cell for forming a sample stream containing particles; a detector for detecting the particle at a first stream to generate a signal representative of the particle;; an imaging device for capturing an image of the particle at a second area of the sample stream; a display; a calculator for calculating a plurality of characteristic parameters of each particle; a distribution preparation device for preparing a distribution of the characteristic parameters to display the distribution on the display; a designation device for previously designating a region in the distribution; a region memory for storing the designated region; a decision device for deciding whether the characteristic parameters of the particle detected at the first area by the detection means are located in the designated region stored in the region memory; an imaging controller for allowing the imaging device to imaging the particle when the characteristic parameters are located in the designated region; an image memory for storing the image of the particle; and a controller for selectively reading the image of the particle to allow the display device to display the read image.

Inventors

  • 👤 Tokihiro Kosaka
  • 👤 Hiroyuki Nakamoto
  • 👤 Fumio Kubota

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-05 22:48
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1456; G01N15/1459

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 227 words
A flowcytometer comprising : a sheath flow cell (3) for forming a sample stream containing particles ; detection means (7, 11) for detecting the particle at a first area of the sample stream to generate a signal representative of the particle ; imaging means (15) for imaging the particle at a second area of the sample stream ; display means (500) ; calculation means (300) for calculating a plurality of characteristic parameters indicating characteristics of each particle based on the generated signal ; distribution preparation means (M) for preparing a distribution of the characteristic parameters and displaying the distribution on the display means ; region designation means (400) for previously designating at least one region in the distribution ; region storage means (202, 203) for storing the designated region ; decision means (205) for deciding whether the characteristic parameters of the particle detected at the first area by the detection means are located in the designated region stored in the region storage means ; imaging control means (200) for allowing the imaging means to image the particle when the characteristic parameters are located in the designated region ; image storage means (600) for storing the image of the particle obtained by the imaging means ; and displaying control means for selectively reading the image of the particle to allow the display means to display the read image.
Claim 2
Dependent Claim 80 words
A flowcytometer of claim 1, wherein the distribution preparation means (M) prepares a plurality of distributions based on the characteristic parameters designated by parameter designation means and displays the plurality of distributions on the displaying means, the region designation means (400) previously designates a region in each distribution, the imaging means (15) images the particle when the plurality of characteristic parameters for the particle detected by the detection means are located in the designated regions of the plurality of distributions.
Claim 3
Dependent Claim 54 words
A flowcytometer of claim 1, wherein the region designation means (400) designates a plurality of regions in a distribution, the imaging means (15) images the particle when the plurality of characteristic parameters for the particle detected by the detection means are located in any one of the designated regions of the plurality of distributions.
Claim 4
Dependent Claim 69 words
A flowcytometer of claim 1, wherein the region storage means (202, 203) stores the designated region by allowing memory addresses thereof to correspond to the characteristic parameters so that data bits inside the designated region and data bits outside the designated region are represented by different binary data, and the decision means (205) decides whether the characteristic parameters are located in the designated region based on the binary data.
Claim 5
Dependent Claim 30 words
A flowcytometer of claim 1 further comprising means for presetting the number of imaging times of the imaging means with respect to each region designated by the region designation means.
Claim 6
Dependent Claim 39 words
A flowcytometer of claim 1, wherein the imaging control (200) means comprising triggering signal generating means (210) for generating a signal every time the particle reaches the second area based on the generated signal to trigger the imaging means.
Claim 7
Dependent Claim 59 words
A flowcytometer of claim 1, further comprising coordinate designation means for designating a coordinate point in the distribution, wherein the displaying control means, when the coordinate designation means designates a coordinate point corresponding to the imaged particle, reads the image of the particle corresponding to the designated coordinate point to allow the display means to display the read image.
Claim 8
Dependent Claim 23 words
A flowcytometer of claim 7, wherein the coordinate point corresponding to the imaged particle is represented with a first identifier in the distribution.
Claim 9
Dependent Claim 23 words
A flowcytometer of claim 7, wherein the coordinate point corresponding to the displayed particle is represented with a second identifier in the distribution.

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