EP0856586A1
Expired / Lapsed
EP
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Biosensor and method of manufacturing the same
Assignee: Panasonic Holdings Corp
•
Filing: 1998-01-15
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Granted: —
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Expires: 2018-01-15
Abstract
Abstract Disclosed is a biosensor facilitating high accuracy quantification of a specific component in a sample solution with no adverse influence of solid substances. The biosensor comprises an electrically insulating base plate, an electrode system comprising a working electrode and a counter electrode formed on the base plate, a reaction layer containing at least one enzyme disposed on the electrode system, and an anionic filter formed on the reaction layer for inhibiting permeation of solid components.
Inventors
- 👤 Yoshiko Miyamoto
- 👤 Shin Ikeda
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 06:18
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/26
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
52 words
A biosensor comprising an electrically
insulating base plate, an electrode system having a
working electrode and a counter electrode formed over said
base plate, a reaction layer containing at least an enzyme
disposed on said electrode system, and an anionic filter
formed over said reaction layer for inhibiting permeation
of solid components.
Claim 2
Dependent Claim
38 words
The biosensor in accordance with claim 1,
wherein said anionic filter is composed of a porous film
made of a film-forming polymer or a fiber sheet, and an
anionic polymer supported on said porous film or fiber
sheet.
Claim 3
Dependent Claim
27 words
The biosensor in accordance with claim 1,
wherein said anionic filter is a porous film made of a
mixture of a film-forming polymer and an anionic polymer.
Claim 4
Dependent Claim
82 words
The biosensor in accordance with claim 2 or
3, wherein said film-forming polymer is at least one
selected from the group consisting of ethyl cellulose,
methyl cellulose, hydroxypropyl cellulose, cellulose
acetate, nitrocellulose, polyvinyl pyrrolidone,
polysulfon, polyvinylidene fluoride, polyamide and
polyimide, and said anionic polymer is at least one
selected from the group consisting of polymers having at
the side chain thereof at least one functional group
selected from the group consisting of a sulfonyl group, a
sulfonate group and a carboxyl group.
Claim 5
Independent Claim
79 words
A method of manufacturing a biosensor
comprising the steps of: dropping a solution containing a hydrophilic
polymer on an electrode system disposed on an electrically
insulating base plate and drying said solution to form a
hydrophilic polymer layer on said electrode system, dropping a solution containing at least one
enzyme on said hydrophilic polymer layer and drying the
solution to form a reaction layer on said hydrophilic
polymer layer, and forming an anionic filter for covering said
reaction layer.
Claim 6
Dependent Claim
75 words
The method of manufacturing a biosensor in
accordance with claim 5, wherein the step of forming said
anionic filter comprises the steps of: disposing a medium dissolved or dispersed
therein with a film-forming polymer on said reaction layer
and drying the medium to form a filter, and impregnating said filter with a medium dissolved
or dispersed therein with an anionic polymer and drying
the filter to form an anionic filter imparted with an
anionic property.
Claim 7
Dependent Claim
30 words
The method of manufacturing a biosensor in
accordance with claim 6, wherein said medium for
dissolving or dispersing said anionic polymer is one that
would not dissolve said film-forming polymer.
Claim 8
Dependent Claim
51 words
The method of manufacturing a biosensor in
accordance with claim 5, wherein the step of forming said
anionic filter comprises the step of disposing a mixture
dissolved or dispersed therein with a film-forming polymer
and an anionic polymer on said reaction layer and drying
the mixture to form an anionic filter.
Claim 9
Dependent Claim
66 words
The method of manufacturing a biosensor in
accordance with claim 5, wherein the step of forming said
anionic filter comprises the steps of: adhering with pressure a fiber sheet onto said
reaction layer to form a filter, and impregnating said filter with a medium dissolved
or dispersed therein with an anionic polymer and drying
the filter to obtain an anionic filter imparted with an
anionic property.
Claim 10
Dependent Claim
34 words
The method of manufacturing a biosensor in
accordance with claim 5, wherein the step of forming said
anionic filter comprises the step of adhering with
pressure a pre-fabricated anionic filter onto said
reaction layer.
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